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301.
Itoh Takeshi; Aiba Hiroji; Baba Tomoya; Hayashi Kouji; Inada Toshifumi; Isono Katumi; Kasai Hiroaki; Kimura Shigenobu; Kitakawa Madoka; Kitagawa Masanari; Makino Kozo; Miki Takeyoshi; Mizobuchi Kiyoshi; Mori Hirotada; Mori Tomoko; Motomura Kouji; Nakade Shinsuke; Nakamura Yoshikazu; Nashimoto Hiroko; Nishio Yoshitaka; Oshima Taku; Saito Noriko; Sampei Gen-ichi; Seki Yasushi; Sivasundaram Suharnan; Tagami Hideaki; Takeda Jun-ichi; Takemoto Keiko; Wada Chieko; Yamamoto Yoshihiro; Horiuchi Takashi 《DNA research》1996,3(6):379-392
The 465,813 base pair sequence corresponding to the 40.150.0min region on the genetic map of Escherichia coli K-12 (W3110)was determined. Analysis of the sequence revealed that thisregion contained at least 466 potential open reading frames,of which 187 (40%) were previously reported, 105 (23%) werehomologous to other known genes, 103 (22%) were identical orsimilar to hypothetical genes registered in databases, and theremaining 71 (15%) did not show a significant similarity toany other gene. At the 45.246.0 min region, we founda very large cluster of about 30 genes, whose functions areinvolved in the biosynthesis of polysaccharides as the componentsof outer membranes. In addition, we identified anew asn-tRNAgene, designated asnW, between the asnT and asnU genes and anew lysogenic phage attachment site as the cis-element. 相似文献
302.
303.
Tohru Mizushima Yoshihisa Ohtsuka Takeyoshi Miki Kazuhisa Sekimizu 《FEMS microbiology letters》1994,121(3):333-336
Abstract We previously reported that plasmid DNA in Escherichia coli cells growing under aerobic conditions relaxed immediately and transiently after heat shock (Mizushima, T., Natori, S. and Sekimizu, K., Mol. Gen. Genet. (1993) 238, 1–5). We next examined DNA relaxation and induction of heat shock proteins after heat shock in cells growing under anaerobic conditions. DNA in these cells relaxed rapidly (2 min) after heat shock (42°C), as was the case with aerobically growing cells, but full superhelicity was not recovered. The relaxed state of DNA topology was maintained for 60 min after heat shock. Induction of DnaK and GroEL proteins, which was transient in aerobically growing cells, was continuous in anaerobically growing cells. Therefore, induction of heat shock proteins correlated with DNA relaxation in both aerobic and anaerobic conditions. 相似文献