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401.
Ei-Tora Yamamura Michio Sayama Makiko Kakikawa Masa-aki Mori Akira Taketo Ken-Ichi Kodaira 《Biochimica et Biophysica Acta (BBA)/General Subjects》2000,1475(1):10-16
The N-hydroxyarylamine O-acetyltransferase of Escherichia coli has been expressed as a histidine tagged fusion protein and purified using immobilized nickel column chromatography. The molecular mass of the histidine tagged N-hydroxyarylamine O-acetyltransferase was estimated to be 60.0 kDa by gel filtration and 34.0 kDa by SDS–PAGE and DNA sequence, suggesting that the native enzyme exists as homo dimer. The catalytic properties were investigated using o-aminobenzoic acid as a substrate. No difference in acetyltransfer activity was observed between histidine tagged protein and untagged enzyme. Kinetic studies indicated a ping-pong bi bi mechanism of the catalysis. Inhibition by N-ethylmaleimide and salicylic acid was competitive with o-aminobenzoic acid and non-competitive with acetyl-CoA. 相似文献
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A Taketo 《Journal of biochemistry》1989,105(5):813-817
Efficient and reproducible DNA-transfection was attained in E. coli, by electroporation. The yield of the transfectants was affected by pretreatment of the recipient cells as well as by the composition of the electroporation medium. Using a single pulse procedure, relationships among the electrical parameters, the transfection efficiency, and the cellular viability were investigated in 10 mM Tris-HCl buffer (pH 7.5) containing 5% sucrose. Certain sodium salts (e.g., citrate, phosphate, and sulfate) were promotive, whereas Mg2+, DEAE-dextran, and polyvinylpyrrolidone were inhibitory to the transfection. Heterologous nucleic acids (native DNA, denatured DNA, and tRNA) exerted only a marginal effect on transfection with a viral replicative-form DNA. The efficiency of DNA transfer was affected by culture conditions, and bacteria grown at a higher temperature were more competent. The electroporation system was more efficient than an improved CaCl2 method, not only in transfection with viral single- and double-stranded DNAs, but also in transformation with plasmid DNAs. 相似文献
406.
Takumi Itoh Ryo Hatano Yoshiya Horimoto Taketo Yamada Dan Song Haruna Otsuka Yuki Shirakawa Shuji Mastuoka Noriaki Iwao Thomas M. Aune Nam H. Dang Yutaro Kaneko Ko Okumura Chikao Morimoto Kei Ohnuma 《Cell death & disease》2021,12(6)
Triple-negative breast cancer (TNBC) has a poor prognosis compared to other breast cancer subtypes. Although epidermal growth factor receptor (EGFR) is overexpressed in TNBC, clinical trials with EGFR inhibitors including tyrosine kinase inhibitors (EGFR-TKI) in TNBC have heretofore been unsuccessful. To develop effective EGFR-targeted therapy for TNBC, the precise mechanisms of EGFR-TKI resistance in TNBC need to be elucidated. In this study, to understand the molecular mechanisms involved in the differences in EGFR-TKI efficacy on TNBC between human and mouse, we focused on the effect of IL-26, which is absent in mice. In vitro analysis showed that IL-26 activated AKT and JNK signaling of bypass pathway of EGFR-TKI in both murine and human TNBC cells. We next investigated the mechanisms involved in IL-26-mediated EGFR-TKI resistance in TNBC. We identified EphA3 as a novel functional receptor for IL-26 in TNBC. IL-26 induced dephosphorylation and downmodulation of EphA3 in TNBC, which resulted in increased phosphorylation of AKT and JNK against EGFR-TKI-induced endoplasmic reticulum (ER) stress, leading to tumor growth. Meanwhile, the blockade of IL-26 overcame EGFR-TKI resistance in TNBC. Since the gene encoding IL-26 is absent in mice, we utilized human IL-26 transgenic (hIL-26Tg) mice as a tumor-bearing murine model to characterize the role of IL-26 in the differential effect of EGFR-TKI in human and mice and to confirm our in vitro findings. Our findings indicate that IL-26 activates the bypass pathway of EGFR-TKI, while blockade of IL-26 overcomes EGFR-TKI resistance in TNBC via enhancement of ER stress signaling. Our work provides novel insights into the mechanisms of EGFR-TKI resistance in TNBC via interaction of IL-26 with its newly identified receptor EphA3, while also suggesting IL-26 as a possible therapeutic target in TNBC.Subject terms: Stress signalling, Cell death and immune response, Breast cancer 相似文献
407.
Sensitivity of Escherichia coli to viral nucleic acid. V. Competence of calcium-treated cells 总被引:13,自引:0,他引:13
A Taketo 《Journal of biochemistry》1972,72(4):973-979
408.
This paper unfolds the events, the people and the times that led up to the
founding of AChemS and fashioned its character during its early formative
years. It describes the path over which AChemS came, going from the
original assertions and denials for the need of such an organization to its
later inception and nascent development. This narration highlights such
topics as the debate over the need for AChemS, the role of National Science
Foundation in the founding of AChemS, the derivation of the Association's
name, the choice of Sarasota and the Hyatt House as the meeting site, the
generation of the programs for the early annual meetings, the adoption of
the bylaws, the process of incorporation and tax deferment, and the birth
of the Givaudan Lectureship. Most emphatically highlighted, however, is the
enthusiasm, commitment and hard work that the members of the chemosensory
research community displayed in bringing AChemS to fruition.
相似文献
409.