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971.
Address correspondence and offprint requests to: T. Sasazuki. 相似文献
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Dextransucrase (EC. 2.4.1.5) of Leuconostoc mesenteroides was purified from the culture filtrate by precipitation with solid ammonium sulfate in the presence of egg white albumin followed by successively treating with columns of DEAE-cellulose and Bio Gel P-150.The purified enzyme lost the activity upon dialysis against EDTA, and was reactivated by the addition of alkaline arth metal ions. The best reactivation was brought about by calcium ion. The enzyme inactivated by EDTA was unstable and readily denatured irreversibly. Several other properties of the purified enzyme were also investigated and discussed. 相似文献
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Kai Wang Ryo Tomura Wei Chen Miho Kiyooka Hinako Ishizaki Tomoyuki Aizu Yohei Minakuchi Masahide Seki Yutaka Suzuki Tatsuya Omotezako Ritsuko Suyama Aki Masunaga Charles Plessy Nicholas M. Luscombe Christelle Dantec Patrick Lemaire Takehiko Itoh Atsushi Toyoda Hiroki Nishida Takeshi A. Onuma 《Development, growth & differentiation》2020,62(6):450-461
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978.
Takehiko Yamanaka Koichi Tanaka Akira Otuka Ottar N. Bjørnstad 《Ecological Research》2007,22(2):185-196
We observed a weed (Ambrosia artemissifolia)–beetle herbivore (Ophraella communa) system for three years in a spatially continuous field (≈200 ha). We analyzed our field data in the light of two contrasting
theories: the resource-concentration hypothesis and reaction–diffusion theory. For the resource-concentration hypothesis, we calculated the correlation coefficients between weed and beetle abundances
for every season in each year. Although we found weak support for resource concentration in some seasons, we could not find
any clear relationships in other seasons. We discuss a dispersal-based mechanism to explain the differences observed among
seasons in lieu of the resource-concentration hypothesis. For the reaction–diffusion theory, we estimated the nonparametric
spatial covariance functions for the spatial autocorrelation of weeds and beetles. Although we could not find any strong spatial
structure for the individual species, we found evidence of spatial interactions between weeds and beetles using time lagged
cross-correlation functions. Weed abundance enhanced local beetle abundance. Through time, there was evidence of beetle spillover
to adjacent locations at roughly the one beetle-generation time scale. Sites with large number of beetles did not seem to
reduce subsequent weed abundance. 相似文献
979.
Synovial Mononuclear Cells Consist with T Cells Which Produce High Levels of Tumor Necrosis Factor α
Hidenobu Akatsuka Mitsuo Okubo Hironori Ishida Katsumi Chiba Kenichi Imanishi Takehiko Uchiyama Kazuhiko Yamamoto Reiji Kasukawa Tomoe Nishimaki 《Microbiology and immunology》1997,41(4):367-370
To determine whether synovial mononuclear cells include a population of tumor necrosis factor α-produeing T cells, we measured tumor necrosis α levels in culture supernatants of synovial mononuclear cells by ELISA and analyzed tumor necrosis α mRNA-positive cell frequencies. There were no significant differences in the spontaneous levels of TNF α between synovial mononuclear cells and peripheral mononuclear cells. The frequency of tumor necrosis factor α mRNA-positive cells in synovial mononuclear cells was higher than that of peripheral mononuclear cells. When stimulated with a superantigen, mononuclear cells from the synovial fluid of rheumatoid arthritis patients showed higher levels of tumor necrosis factor α production (1,035 ± 817 pg/ml) than did mononuclear cells from their peripheral blood (236 ± 180 pg/ml). In addition, we observed that a few T cell clones were resistant to superantigenic restimulation in vitro. We conclude that when these types of T cells persist in the synovium, they play a role in the development of rheumatoid arthritis via a mechanism involving tumor necrosis factor α production. 相似文献