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81.
Y Hitoshi N Yamaguchi S Mita E Sonoda S Takaki A Tominaga K Takatsu 《Journal of immunology (Baltimore, Md. : 1950)》1990,144(11):4218-4225
mAb to murine IL-5R were prepared by means of fusion between mouse myeloma cells and spleen cells from a rat immunized with membrane-enriched fractions of IL-5-dependent early B cell line (T88-M). Two mAb (H7 and T21) were selected for their competitive inhibition of receptor binding by 35S-labeled IL-5 and of IL-5 biologic activities. The number of binding sites recognized by the mAb on different cell lines correlated with IL-5 responsiveness. Most surface IgM+ peritoneal B cells were H7+ and more than 70% were also Ly-1(CD5)dull+, and responded to IL-5 for polyclonal IgM production in a high frequency. A significant proportion of splenic B cells reacted with these mAb, although lower number (one-log less) than peritoneal B cells and a small proportion of H7dull+ splenic B cells seems to be Ly-1(CD5)dull+, 1 of 200 splenic B cells responded to IL-5 for IgM production. These results suggest that IL-5R+ B cells may consist of a subpopulation of B cells. Intriguingly, lymphoid populations of bone marrow cells were stained with H7 and T21, whereas myeloid populations were brightly stained with only T21. Finally, both H7 and T21 mAb specifically precipitated a protein of a Mr 60,000 from 125I-labeled cell lysates of IL-5R+ T88-M cells. The IL-5R with similar size (Mr 55,000 to 60,000) was precipitated from the cell lysates of peritoneal B cells. T21 mAb but not H7 mAb precipitated a protein of a Mr 110,000 from the cell lysates of bone marrow cells. 相似文献
82.
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84.
Takaki Fukuizumi Hiromasa Inoue Yuichi Anzai Toshiyuki Tsujisawa Choji Uchiyama 《Microbiology and immunology》1995,39(5):351-359
We have shown that the palatine tonsil effectively incorporates exogenous foreign substances instilled at its surface. It is not clear whether antigen-specific IgA can be induced by the instillation. Sheep red blood cells (SRBC) were instilled at the palatine tonsil every three days as the antigen, and the agglutination titer of specific IgA in saliva was examined. Nasal or intragastric administration, which have been shown to induce specific antibody in saliva, were done as control experiments. Anti-SRBC antibody in saliva from the tonsillar instillation group was detected in the second week, and the agglutination titer reached a maximum in the 6th week after the instillation. The maximum titers in the tonsillar instillation group and nasal administration group were 16 (P<0.01, n=7) and 4 times (P<0.01, n = 7) higher, respectively, than that in the intragastric administration group. In the tonsillar instillation group, the number of specific antibody-producing cells per 105 lymphocytes was the highest in the parotid glands compared with the lymphoid tissues such as the retropharyngeal lymph nodes, nasal mucosa, mesenteric lymph nodes, Peyer's patches, cervical lymph nodes, palatine tonsil and spleen. In the nasal administration group, the number of lymphocytes was the highest in the nasal mucosa. The results indicate that tonsillar instillation was more effective than nasal administration in inducing specific IgA in saliva. 相似文献
85.
Habitat preference of juveniles ofLates angustifrons andLates mariae, endemic fish species in Lake Tanganyika, was investigated in the field and in the laboratory. Both species settle on the
grass bed after the post-larval pelagic phase.L. angustifrons preferred the short grass bed composed ofVallismeria sp., whileL. mariae preferred the tall grass bed composed ofPotamogeton schweinfurthii. L. angustifrons always rested near the root, keeping a position parallel to the bottom; dark cross bands on the body may be advantageous
as camouflage in the short grass bed. On the other hand,L. mariae always stayed in mid-water, keeping a head-down position parallel to a leaf or stem of grass; such posture and longitudinal
stripes on the body may be advantageous as camouflage in the tall grass bed. Both species were more active at night, and fed
mainly on atyid shrimps.Lates angustifrons shifted its main food from shrimps to fish with growth, as was suggested inL. mariae. Difference in the habitat preference may play an important role in their coexistence. 相似文献
86.
B. Hossain N. Hirata Y. Nagatomo R. Akashi H. Takaki 《Journal of Plant Growth Regulation》1997,16(4):239-243
A high zinc concentration of 520 μm, approximately 100 times that used most often in standard plant tissue culture media, was found to be superior in liquid
callus cultures of japonica rice, increasing growth to 146% compared with standard N6 medium. At the same time, the internal
zinc concentration increased 40 times in fast growing cells; soluble protein doubled, and free amino acids decreased. Under
zinc-free conditions the cultures slowed in growth, and several free amino acids such as aspartic acid, glutamic acid, asparagine,
and glutamine accumulated. We suggest that zinc acts as a direct regulatory factor in inducing auxin activity, but not auxin
levels, making high internal zinc accumulation mandatory if high auxin concentrations are required as in rice callus cultures.
Received July 16, 1997; accepted September 22, 1997 相似文献
87.
Sayaka Katsunuma Hisao Honda Tomoyasu Shinoda Yukitaka Ishimoto Takaki Miyata Hiroshi Kiyonari Takaya Abe Ken-ichi Nibu Yoshimi Takai Hideru Togashi 《The Journal of cell biology》2016,212(5):561-575
In the olfactory epithelium (OE), olfactory cells (OCs) and supporting cells (SCs), which express different cadherins, are arranged in a characteristic mosaic pattern in which OCs are enclosed by SCs. However, the mechanism underlying this cellular patterning is unclear. Here, we show that the cellular pattern of the OE is established by cellular rearrangements during development. In the OE, OCs express nectin-2 and N-cadherin, and SCs express nectin-2, nectin-3, E-cadherin, and N-cadherin. Heterophilic trans-interaction between nectin-2 on OCs and nectin-3 on SCs preferentially recruits cadherin via α-catenin to heterotypic junctions, and the differential distributions of cadherins between junctions promote cellular intercalations, resulting in the formation of the mosaic pattern. These observations are confirmed by model cell systems, and various cellular patterns are generated by the combinatorial expression of nectins and cadherins. Collectively, the synergistic action of nectins and cadherins generates mosaic pattern, which cannot be achieved by a single mechanism. 相似文献
88.
Ethylene‐dependent aerenchyma formation in adventitious roots is regulated differently in rice and maize 下载免费PDF全文
Takaki Yamauchi Akihiro Tanaka Hitoshi Mori Itsuro Takamure Kiyoaki Kato Mikio Nakazono 《Plant, cell & environment》2016,39(10):2145-2157
In roots of gramineous plants, lysigenous aerenchyma is created by the death and lysis of cortical cells. Rice (Oryza sativa) constitutively forms aerenchyma under aerobic conditions, and its formation is further induced under oxygen‐deficient conditions. However, maize (Zea mays) develops aerenchyma only under oxygen‐deficient conditions. Ethylene is involved in lysigenous aerenchyma formation. Here, we investigated how ethylene‐dependent aerenchyma formation is differently regulated between rice and maize. For this purpose, in rice, we used the reduced culm number1 (rcn1) mutant, in which ethylene biosynthesis is suppressed. Ethylene is converted from 1‐aminocyclopropane‐1‐carboxylic acid (ACC) by the action of ACC oxidase (ACO). We found that OsACO5 was highly expressed in the wild type, but not in rcn1, under aerobic conditions, suggesting that OsACO5 contributes to aerenchyma formation in aerated rice roots. By contrast, the ACO genes in maize roots were weakly expressed under aerobic conditions, and thus ACC treatment did not effectively induce ethylene production or aerenchyma formation, unlike in rice. Aerenchyma formation in rice roots after the initiation of oxygen‐deficient conditions was faster and greater than that in maize. These results suggest that the difference in aerenchyma formation in rice and maize is due to their different mechanisms for regulating ethylene biosynthesis. 相似文献
89.
Suge Hiroshi; Takahashi Hideyuki; Arita Shigeaki; Takaki Hiroshi 《Plant & cell physiology》1986,27(6):1005-1012
The content of gibberellin-like substances was reduced to aboutone seventh in maize and one half in barley and oat plants grownunder zinc-deficient conditions compared with control plantsgrown with sufficient zinc nutrition. The supplement of zincto zinc-deficient maize plants not only recovered their growthbut also increased contents of gibberellin-like substances.The growth cessation in zinc-deficient plants is discussed inrelation to the endogenous contents of gibberellin. (Received March 12, 1986; Accepted May 28, 1986) 相似文献
90.
Rat pancreatic islets cultured for 6 days at 100 or 500 mg/dl glucose and 20 or 7% O2 were examined electron-microscopically, and insulin accumulation in the culture media was assayed immunologically. In the islets cultured at 500 mg/dl glucose and 20% O2, B cells exhibited hypertrophy of granular endoplasmic reticulum and Golgi apparatus, an abundance of free ribosomes, degranulation and the margination of secretory granules. In islets cultured at 500 mg/dl glucose and 7% O2, B cells exhibited dilatation of endoplasmic reticulum cisternae and dominance of Golgi vesicles in addition to the above-mentioned changes. These changes, together with the correlated data on insulin accumulation, are discussed with special reference to the effects of glucose and oxygen upon the synthesis and release of insulin in B cells. 相似文献