l-Threo-γ-hydroxycitrulline from Vicia pseudo-orobus

l-Threo-γ-hydroxycitrulline was isolated and identified from the seeds of Vicia pseudo-orobus. The structure was clarified from the resul     

Cardiac Arrest during Gamete Release in Chum Salmon Regulated by the Parasympathetic Nerve System

Cardiac arrest caused by startling stimuli, such as visual and vibration stimuli, has been reported in some animals and could be considered as an extraordinary case of bradycardia and defined as reversible missed heart beats. Variability of the heart rate is established as a balance between an autonomic system, namely cholinergic vagus inhibition, and excitatory adrenergic stimulation of neural and hormonal action in teleost. However, the cardiac arrest and its regulating nervous mechanism remain poorly understood. We show, by using electrocardiogram (ECG) data loggers, that cardiac arrest occurs in chum salmon (Oncorhynchus keta) at the moment of gamete release for 7.39±1.61 s in females and for 5.20±0.97 s in males. The increase in heart rate during spawning behavior relative to the background rate during the resting period suggests that cardiac arrest is a characteristic physiological phenomenon of the extraordinarily high heart rate during spawning behavior. The ECG morphological analysis showed a peaked and tall T-wave adjacent to the cardiac arrest, indicating an increase in potassium permeability in cardiac muscle cells, which would function to retard the cardiac action potential. Pharmacological studies showed that the cardiac arrest was abolished by injection of atropine, a muscarinic receptor antagonist, revealing that the cardiac arrest is a reflex response of the parasympathetic nerve system, although injection of sotalol, a β-adrenergic antagonist, did not affect the cardiac arrest. We conclude that cardiac arrest during gamete release in spawning release in spawning chum salmon is a physiological reflex response controlled by the parasympathetic nervous system. This cardiac arrest represents a response to the gaping behavior that occurs at the moment of gamete release.     

Statistical properties of the Jukes-Holmquist method of estimating the number of nucleotide substitutions: Reply to Holmquist and Conroy's criticism

Summary Conducting computer simulations, Nei and Tateno (1978) have shown that Jukes and Holmquist's (1972) method of estimating the number of nucleotide substitutions tends to give an overestimate and the estimate obtained has a large variance. Holmquist and Conroy (1980) repeated some parts of our simulation and claim that the overestimation of nucleotide substitutions in our paper occurred mainly because we used selected data. Examination of Holmquist and Conroy's simulation indicates that their results are essentially the same as ours when the Jukes-Holmquist method is used, but since they used a different method of computation their estimates of nucleotide substitutions differed substantially from ours. Another problem in Holmquist and Conroy's Letter is that they confused the expected number of nucleotide substitution with the number in a sample. This confusion has resulted in a number of unnecessary arguments. They also criticized ourX ₂ measure, but this criticism is apparently due to a misunderstanding of the assumptions of our method and a failure to use our method in the way we described. We believe that our earlier conclusions remain unchanged.     

Relative roles of mutation and selection in the maintenance of genetic variability

The extent and pattern of protein and DNA polymorphisms are discussed with emphasis on the mechanism of maintenance of the polymorphisms. Statistical studies suggest that a large proportion of genetic variability at the molecular level is maintained by a mutation-drift balance. At some loci, such as those for histocompatibility in mammals, however, a form of overdominant selection seems to be involved. In the presence of overdominant selection, polymorphic alleles may be maintained for tens of millions of years, so that the number of nucleotide differences between alleles is often very large, as in the case of self-incompatibility alleles in plants. There are also an increasing number of examples in which an adaptive change of a morphological or physiological character is caused by a single nucleotide substitution. Nevertheless, these mutations seem to be a small proportion of the total nucleotide changes that contribute to genetic variability and evolution. Although there are many examples of frequency-dependent selection, this form of selection is apparently unimportant for the maintenance of genetic variability except in some special cases. Observations on the evolutionary change of DNA suggest that the driving force of evolution is mutation rather than selection.     

Standard error of immunological dating of evolutionary time

Summary The empirical variance of the immunological distance as measured by microcomplement fixation with albumin is determined. The variance obtained is at least two times larger than the mean when the mean is small and the ratio of the variance to the mean increases with increasing mean. Thus, the immunological dating of evolutionary time has a large standard error. It is shown that in bird lysozymes the relationship between immunological distance (y) and the number of amino acid substitutions per 100 sites (x) is given byy = 4.2x approximately.     

An update on the phylogeny of Spintharinae with analysis based on morphological characters and taxonomy of Janula (Araneae,Theridiidae)

Organisms Diversity & Evolution - The present study evaluates the monophyly of Spintharinae with the inclusion of all ten genera listed under subfamily (Brunepisinus; Chrosiothes; Episinus;...