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131.
The effects of compactin, a specific inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase, on the growth of alfalfa seedlings in vivo and the rhizogenesis of pepper explants in vitro were investigated. Compactin added to the agar medium inhibited the elongation of roots and hypocotyls of etiolated alfalfa seedlings. The growth inhibition was accompanied by strict inhibition of sterol synthesis. Addition of mevalonic acid, the direct product of 3-hydroxy-3-methylglutaryl coenzyme A reductase, together with compactin relieved the growth inhibition. The sterol level in the seedlings was also protected against the lowering effect of compactin. Similarly, the rhizogenetic process of cultured explants of pepper was inhibited by compactin and relieved by mevalonic acid. Several isoprenoid end products were tested in combination with compactin to determine which compounds, if any, might be limiting for growth. Exogenously supplied isoprenoids failed to relieve the growth inhibition of seedlings. In contrast, they partly relieved the growth inhibition of explants, suggesting their important role in plant growth. During the course of these experiments, it was also found that brassinolide caused remarkable growth inhibition and twisting of alfalfa seedlings. 相似文献
132.
133.
Yoshinori Toyoshima Kozo Akabori Akiko Imaoka Haruto Nakayama Nozomu Ohkouchi Asako Kawamori 《FEBS letters》1984,176(2):346-350
Extraction of PS II particles with 1 M CaCl2 caused complete disappearance of the light-induced signal of the possible Kok S2 state of the water-splitting complex and total loss of the O2, evolving activity, concomitant with perfect removal of the 17-, 23- and 34-kDa proteins from the particles. The recovery of the multiline signal in the CaCl2-treated PS II was performed by reinserting the 34-kDa protein, when CI? was present in the solution for the EPR measurement. However, in the absence of Cl?, besides the 34-kDa protein, the 17- and 23-kDa proteins were required for the recovery of the signal. These results are compared with the results on the recovery of the O2, evolution in the reconstituted PS II to examine the role of these three proteins on the water splitting. 相似文献