全文获取类型
收费全文 | 1283篇 |
免费 | 72篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 20篇 |
2020年 | 12篇 |
2019年 | 7篇 |
2018年 | 30篇 |
2017年 | 16篇 |
2016年 | 28篇 |
2015年 | 45篇 |
2014年 | 55篇 |
2013年 | 78篇 |
2012年 | 111篇 |
2011年 | 94篇 |
2010年 | 44篇 |
2009年 | 49篇 |
2008年 | 93篇 |
2007年 | 83篇 |
2006年 | 83篇 |
2005年 | 71篇 |
2004年 | 73篇 |
2003年 | 66篇 |
2002年 | 71篇 |
2001年 | 12篇 |
2000年 | 17篇 |
1999年 | 14篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 12篇 |
1992年 | 9篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 14篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1973年 | 3篇 |
排序方式: 共有1355条查询结果,搜索用时 31 毫秒
21.
Michiyoshi Masuda Kimio Isiglo Hideki Yamoka Takafumi Matsumoto Manabu Takahashi 《Biochemical and biophysical research communications》1984,119(3):920-925
The fate of 3H-thymidine incorporated into newly synthesized DNA of CHO cells was analyzed by either the estimation of the incorporated radioactivity per cell or sedimentation in alkaline sucrose gradient. Under conditions in which DNA synthesis proceeded continuously, of incorporated radioactivity was periodically lost and regained during a 90 min chase, corresponding to a cyclic change in the sedimentation profiles. When DNA synthesis was inhibited by hydroxyurea no cyclic change of the incorporated radioactivity was observed. The cyclic changes were regarded as the result of an actual metabolic change in3H-labelled DNA probaly joining to one of the newly formed sister strands of DNA and the loss of radioactivity seems to require active continued DNA synthesis. 相似文献
22.
Peter J. Davies Eve Emshwiller Thomas J. Gianfagna William M. Proebsting Masana Noma Richard P. Pharis 《Planta》1982,154(3):266-272
The gibberellins (GAs) of both vegetative (leaves and stems) and reproductive (pods and seeds) tissue of the G2 strain of peas Pisum sativum L. were characterized in purified extracts by a combination of sequential silicic-acid partition column chromatography, and gas chromatography-mass spectrometry. Gibberellins A19, A20, A29 and an A29 catabolite were identified in both types of tissue. Gibberellins A9, A17 and A44 were also found in pods and seeds.Abbreviations FID
Ilame ionization detector
- GA(s)
gibberellin(s)
- GC
gas chromatograph(y)
- HPLC
high performance liquid chromatograph(y)
- LD
long day
- MS
mass spectrum(a) or mass spectrometer(ry)
- SD
short day 相似文献
23.
Y Morimoto T Oishi N Hanasaki A Miyatake K Noma Y Yamamura 《Endocrinologia japonica》1980,27(5):659-666
The relative potency in the hypothalamic-pituitary-adrenal (HPA) suppression of both prednisolone and betamethasone was examined in an acute study with normal volunteers and in a chronic study with glucocorticoid-treated patients. Circadian rhythm of plasma cortisol was studied after a single dose administration of 5 to 30 mg prednisolone or 0.5 to 3.0 mg betamethasone at 8:00 hr. Morning-rise of plasma cortisol occurred on the morning after the administration of 30 mg or less prednisolone but no morning rise was noted after the administration of 1.0 mg or more betamethasone. Plasma ACTH was slightly elevated on the morning after 30 mg prednisolone administration but showed low levels throughout the night after 3.0 mg betamethasone administration. Plasma cortisol responsiveness to ACTH was examined in patients before and during therapy with either prednisolone or betamethasone. The basal cortisol level was not suppressed and the responsiveness to ACTH remained nearly normal during long-term 5 mg prednisolone therapy, but these were completely suppressed during long-term 5 mg betamethasone therapy. The responsiveness to ACTH was nearly normal in patients receiving alternate-day therapy with prednisolone in such large doses as 50 or 60 mg every other day, but was completely suppressed in patients receiving 1.0 mg betamethasone every other day. The relative potency of betamethasone in acute and chronic suppressive effects on the HPA system seems to be much stronger than that of prednisolone in equivalent doses with comparable anti-inflammatory effects. It is also suggested that the alternate-day therapy with such long-acting steroids as betamethasone are useless in preventing HPA suppression. 相似文献
24.
Masato Hirata Takafumi Hamachi Eiichi Suematsu Toshitaka Koga 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1983,763(4)
Effects of N-formyl chemotactic peptides on the Ca2+ influx and efflux were investigated in guinea-pig peritoneal macrophages using an isotope tracer. fMet-Leu-Phe did not enhance the influx of 45Ca2+ into macrophages, whereas it stimulated the efflux of 45Ca2+ from macrophages at concentrations ranging from 10−10 M to 10−7 M. fMet-Met-Met and fMet-Leu also stimulated the 45Ca2+ efflux, albeit at much higher concentrations, while there was no stimulation with fMet. The mitochondrial inhibitors, oligomycin and NaN3, did not modify the 45Ca2+ efflux induced by the chemoattractants, yet they did induce the release of 45Ca2+ from the mitochondria. On the other hand, higher concentrations of the calmodulin antagonists, chlorpromazine and trifluoperazine, induced the release of 45Ca2+ from the NaN3-insensitive Ca2+ store site and mimicked the enhancement of the 45Ca2+ efflux by N-formyl chemotactic peptides. Thus, N-formyl chemotactic peptides appear to increase the levels of intracellular free Ca2+ in guinea-pig peritoneal macrophages, probably by inducing the release of Ca2+ from the NaN3-insensitive Ca2+ store site. 相似文献
25.
Masato Hirata Takafumi Hamachi Eiichi Suematsu Toshitaka Koga 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1983,763(4):339-345
Effects of N-formyl chemotactic peptides on the Ca2+ influx and efflux were investigated in guinea-pig peritoneal macrophages using an isotope tracer. fMet-Leu-Phe did not enhance the influx of 45Ca2+ into macrophages, whereas it stimulated the efflux of 45Ca2+ from macrophages at concentrations ranging from 10?10 M to 10?7 M. fMet-Met-Met and fMet-Leu also stimulated the 45Ca2+ efflux, albeit at much higher concentrations, while there was no stimulation with fMet. The mitochondrial inhibitors, oligomycin and NaN3, did not modify the 45Ca2+ efflux induced by the chemoattractants, yet they did induce the release of 45Ca2+ from the mitochondria. On the other hand, higher concentrations of the calmodulin antagonists, chlorpromazine and trifluoperazine, induced the release of 45Ca2+ from the NaN3-insensitive Ca2+ store site and mimicked the enhancement of the 45Ca2+ efflux by N-formyl chemotactic peptides. Thus, N-formyl chemotactic peptides appear to increase the levels of intracellular free Ca2+ in guinea-pig peritoneal macrophages, probably by inducing the release of Ca2+ from the NaN3-insensitive Ca2+ store site. 相似文献
26.
In a carrot (Daucus carota L.) cell line lacking the ability to undergo somatic embryogenasis, and in carrot and anise (Pimpinella anisum L.) cell lines in which embryogenesis could be regulated by presence or absence of 2,4-dichlorophen-oxyacetic acid (2,4-D), in the medium (+2,4-D=no embryogenesis,-2,4-D=embryo differentiation and development), the levels of endogenous gibberellin(s) (GA) were determined by the dwarfrice bioassay, and the metabolism of [3H]GA1 was followed. Embryos harvested after 14 d of subculture in-2,4-D had low levels (0.2–0.3 g g-1 dry weight) of polar GA (e.g. GA1-like), but much (3–22 times) higher levels of less-polar GA (GA4/7-like); GA1, GA4 and GA7 are native to these cultures. Conversely, the undifferentiated cells in a non-embryogenic strain, and proembryos of an embryogenic strain (+2,4-D) showed very high levels of polar GA (2.9–4.4 g g-1), and somewhat reduced levels of less-polar GA. Cultures of anise undergoing somatic embryo development (-2,4-D) metabolized [3H]GA1 very quickly, whereas proembryo cultures of anise (+2,4-D) metabolized [3H]GA1 slowly. The major metabolites of [3H]GA1 in anise were tentatively identified as GA8-glucoside (24%), GA8 (15%), GA1-glucoside (8%) and the 1(10)GA1-counterpart (2%). Thus, high levels of a GA1-like substance and a reduced ability to metabolize GA1 are correlated with the absence of embryo development, while lowered levels of GA1-like substance and a rapid metabolism of GA1 into GA8 and GA-conjugates are correlated with continued embryo development. Exogenous application of GA3 is known to reduce somatic embryogenesis in carrot cultures; GA4 was found to have the same effect in anise cultures. Thus, a role (albeit negative) in somatic embryogenesis for a polar, biologically active GA is implied.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- GA
gibberellin(s) or gibberellin-like substances
- GC-RC
gas chromatography-radiochromatogram counting
- HPLC
high-presare liquid chromatography
- Rt
retention time
- TLC
thinlaver chromatography 相似文献
27.
Unrolling due to blue light (B) irradiation of the second leavesdetached from 8-day-old rice (Oryza saliva L.) seedlings wassimilar to that reported previously for nondetached leaves.The effect of B was counteracted by irradiation with red light(R). The counteracting effect of R was reversed by subsequentirradiation with far-red light (FR). When the detached leaf was irradiated with B passed througha 1-mm-wide slit 5, 8, 10, 12 or 15 mm from the leaf tip, irradiation10 mm from the leaf tip was the most effective. The effect of a 1 mm-wide-B irradiation 10 mm from the leaftip was counteracted by a 1 mm-wide-R irradiation at the sameposition, but not by irradiations at the other points. The counteractingeffect of R was reversed by a 1 mm-wide-FR irradiation at thesame position. This suggests that the excitation or the reactionof the B photoreceptor(s) is affected directly by the PFR formof phytochrome. The dose-response curve for the unrolling caused by B showeda simple Bunsen-Roscoe relation without two peaks, which differsfrom that for the phototropism in Avena caused by B. (Received August 21, 1980; Accepted December 20, 1980) 相似文献
28.
The physiological action of CCC for leaves of Kyoho grapes wasstudied. Leaves from the lower to upper positions of the primaryshoots and (primary) lateral shoots showed uneven photosyntheticactivities with peaks. The photosynthetic activity of each leaffrom both shoots was increased by treatment with CCC, whereasrespiratory activity was not affected with CCC. In the primaryshoots, CCC treatment increased the contents of chlorophylla and b in the leaves, but in the lateral shoots only chlorophyllb contents were increased. Dry weights of the leaves from thelateral shoots increased with CCC, but those of leaves locatedin the vicinity of nodes bearing the fruit clusters varied slightlyor were almost the same as the dry weight of non-treated leaves.Leaf thickness was increased by CCC and paralleled the variationin dry weight. The leaf area was decreased by CCC. (Received April 19, 1980; ) 相似文献
29.
30.
Summary Active transport of orthophosphate byChlorella ellipsoidea was observed at 25 °C under fluorescent light, about 3 klux. Influx and efflux of phosphate, and extra- and intracellular phosphate concentrations were measured in order to assess phosphate permeability in the cells. The permeability ranged from 10–3 to 10–4
mlQ/mg cell min (or 10–7 to 10–8cm/sec). 相似文献