全文获取类型
收费全文 | 1675篇 |
免费 | 103篇 |
出版年
2023年 | 3篇 |
2022年 | 11篇 |
2021年 | 22篇 |
2020年 | 11篇 |
2019年 | 22篇 |
2018年 | 31篇 |
2017年 | 21篇 |
2016年 | 32篇 |
2015年 | 51篇 |
2014年 | 63篇 |
2013年 | 111篇 |
2012年 | 143篇 |
2011年 | 112篇 |
2010年 | 61篇 |
2009年 | 60篇 |
2008年 | 109篇 |
2007年 | 105篇 |
2006年 | 89篇 |
2005年 | 83篇 |
2004年 | 103篇 |
2003年 | 86篇 |
2002年 | 92篇 |
2001年 | 22篇 |
2000年 | 30篇 |
1999年 | 37篇 |
1998年 | 10篇 |
1997年 | 11篇 |
1996年 | 11篇 |
1995年 | 7篇 |
1994年 | 14篇 |
1993年 | 15篇 |
1992年 | 19篇 |
1991年 | 18篇 |
1990年 | 20篇 |
1989年 | 16篇 |
1988年 | 15篇 |
1987年 | 12篇 |
1986年 | 20篇 |
1985年 | 15篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 8篇 |
1975年 | 5篇 |
1973年 | 4篇 |
1970年 | 3篇 |
1966年 | 2篇 |
排序方式: 共有1778条查询结果,搜索用时 15 毫秒
41.
H Maekawa K Yamazumi S Muramatsu M Kaneko H Hirata N Takahashi N B de Bosch Z Carvajal A Ojeda C L Arocha-Pi?ango 《The Journal of biological chemistry》1991,266(18):11575-11581
We have identified a unique N-glycosylated Asn substitution for a Ser at position 434 of the A alpha chain of an abnormal fibrinogen designated fibrinogen Caracas II. This dysfibrinogen was characterized by impaired fibrin monomer aggregation. Since there were 4 Thr residues immediately following the mutation, a new Asn-X-Thr/Ser-type consensus sequence, Asn-Thr-Thr arose for N-glycosylation of the Asn. The extra oligosaccharide was found to consist mainly of a disialylated biantennary structure comprising 81.9%, while a neutral and a monosialylated biantennary oligosaccharide represented only 3.6% and 14.5%, respectively. The mutation resides in the carboxyl-terminal region of the A alpha chain, which could fold back to form an extra small globular region located near the central region of the molecule (Erickson, H.P., and Fowler, W.E. (1983) Ann. N. Y. Acad. Sci. 408, 146-163; Weisel, H.P., Stauffacher, C.V., Bullitt, E., and Cohen, C. (1985) Science 230, 3124-3133). Therefore, the participation of this region, referred to as an additional central domain or an alpha domain, in fibrin gel formation is strongly implicated. 相似文献
42.
S Hattori N Ohmi M Maekawa M Hoshino M Kawakita S Nakamura 《Biochemical and biophysical research communications》1991,177(1):83-89
Cellular fractionation of GTPase activating protein (GAP) activity using bovine cerebral cortex revealed that about half of GAP activity was found in membrane fraction. GAP activity of membrane was not solubilized with 0.5% (v/v) triton X-100 and was immunoprecipitated with antibody against carboxy-terminus of neurofibromatosis type 1 (NF1) gene product. In contrast, soluble GAP activity was precipitated with antibody against GAP but not with anti-NF1. These results suggest that NF1 gene product is a GTPase activating protein toward ras p21 with completely different intracellular distribution from that of GAP. 相似文献
43.
Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus 总被引:5,自引:0,他引:5
The genes of the BanI restriction-modification system specific for GGPyPuCC were cloned from the chromosomal DNA of Bacillus aneurinolyticus IAM1077, and the coding regions were assigned on the nucleotide sequence on the basis of the N-terminal amino acid sequences and molecular weights of the enzymes. The restriction and modification genes coded for polypeptides with calculated molecular weights of 39,841 and 42,637, respectively. Both the enzymes were coded by the same DNA strand. The restriction gene was located upstream of the methylase gene, separated by 21 bp. The cloned genes were significantly expressed in E. coli cells, so that the respective enzymes could be purified to homogeneity. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration indicated that the catalytically active form of the endonuclease was dimeric and that of the methylase was monomeric. Comparison of the amino acid sequences revealed no significant homology between the endonuclease and methylase, though both enzymes recognize the same target sequence. Sequence comparison with other related enzymes indicated that BanI methylase contains sequences common to cytosine-specific methylases. 相似文献
44.
Unrolling due to blue light (B) irradiation of the second leavesdetached from 8-day-old rice (Oryza saliva L.) seedlings wassimilar to that reported previously for nondetached leaves.The effect of B was counteracted by irradiation with red light(R). The counteracting effect of R was reversed by subsequentirradiation with far-red light (FR). When the detached leaf was irradiated with B passed througha 1-mm-wide slit 5, 8, 10, 12 or 15 mm from the leaf tip, irradiation10 mm from the leaf tip was the most effective. The effect of a 1 mm-wide-B irradiation 10 mm from the leaftip was counteracted by a 1 mm-wide-R irradiation at the sameposition, but not by irradiations at the other points. The counteractingeffect of R was reversed by a 1 mm-wide-FR irradiation at thesame position. This suggests that the excitation or the reactionof the B photoreceptor(s) is affected directly by the PFR formof phytochrome. The dose-response curve for the unrolling caused by B showeda simple Bunsen-Roscoe relation without two peaks, which differsfrom that for the phototropism in Avena caused by B. (Received August 21, 1980; Accepted December 20, 1980) 相似文献
45.
The physiological action of CCC for leaves of Kyoho grapes wasstudied. Leaves from the lower to upper positions of the primaryshoots and (primary) lateral shoots showed uneven photosyntheticactivities with peaks. The photosynthetic activity of each leaffrom both shoots was increased by treatment with CCC, whereasrespiratory activity was not affected with CCC. In the primaryshoots, CCC treatment increased the contents of chlorophylla and b in the leaves, but in the lateral shoots only chlorophyllb contents were increased. Dry weights of the leaves from thelateral shoots increased with CCC, but those of leaves locatedin the vicinity of nodes bearing the fruit clusters varied slightlyor were almost the same as the dry weight of non-treated leaves.Leaf thickness was increased by CCC and paralleled the variationin dry weight. The leaf area was decreased by CCC. (Received April 19, 1980; ) 相似文献
46.
47.
Summary Active transport of orthophosphate byChlorella ellipsoidea was observed at 25 °C under fluorescent light, about 3 klux. Influx and efflux of phosphate, and extra- and intracellular phosphate concentrations were measured in order to assess phosphate permeability in the cells. The permeability ranged from 10–3 to 10–4
mlQ/mg cell min (or 10–7 to 10–8cm/sec). 相似文献
48.
We studied how value for instrumental action is discounted by predicted effort and delay. The monkeys were trained to perform instrumental trials that required a bar release when a visual target changed from red-to-green. There were two trial conditions. In delay trials, after the monkeys performed one instrumental trial correctly a reward was delivered 0–7 seconds later. In work trials, the monkeys had to perform 0, 1, or 2 additional instrumental trials to obtain a reward. The lengths of trials in delay matched the time it took to complete work trials. The length of delay or number of trials was indicated by a visual cue presented throughout the trial. Our hypothesis was that the monkeys would all show temporal discounting of reward in the delay trials, and that in the work trials the monkeys’ performance might reflect an additional cost due to working. The error rate increased linearly as remaining cost increased for all 8 monkeys. For 4 monkeys the error rate was significantly larger in work trials than in delay trials (effort sensitive monkeys). For the other 4 monkeys there was no significant difference in error rate (effort insensitive monkeys). Since the error rate has an inverse relation with value for action, these results suggest that value is discounted hyperbolically by effort as well as by delay. Error rates generally increased as the testing sessions progressed and the total reward accumulated (i.e., effect of reward devaluation). The relative impact of delay and effort on error rates was reasonably stable within subjects. Thus, within the monkey population there seems to be a significant dichotomy in the sensitivity governing whether working is more costly than waiting, possibly arising from a constitutional or genetic trait. 相似文献
49.
50.
Fujishiro Miyuki Yahagi Shoichi Takemi Shota Nakahara Mio Sakai Takafumi Sakata Ichiro 《Molecular biology reports》2021,48(7):5513-5518
Molecular Biology Reports - Pyridoxine (PN), one of the vitamers of vitamin B6, plays an important role in the maintenance of epidermal function and is used to treat acne and rough skin. Clinical... 相似文献