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141.
Shikimate dehydrogenase in mung bean cell suspension culture was partially purified by chromatography on DEAE-cellulose. The
NADP-dependent SDHase was eluted as a single peak, while the NAD-dependent one exhibited two peaks. Polyacrylamide gel electrophoresis
revealed six NAD-dependent isozymes of SDHase, two of which corresponded to NADP-dependent ones. 相似文献
142.
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144.
A Asari S Miyauchi K Miyazaki A Hamai K Horie T Takahashi T Sekiguchi A Machida K Kohno Y Uchiyama 《The journal of histochemistry and cytochemistry》1992,40(11):1693-1704
To demonstrate the intra- and extracellular localization of hyaluronic acid (HA) in articular cartilage of the rabbit tibia, biotinylated HA binding region, which specifically binds to the HA molecule, was applied to the tissue. In comparison with the localization of HA, that of chondroitin sulfate (CS), keratan sulfate (KS), and the protein core (PC) of the proteoglycan was examined by immunohistochemistry. Strong positive staining for HA was detected in chondrocytes located in the transition between the superficial and middle zones of the tissue. Pre-treatment with chondroitinase ABC, keratanase II, or trypsin enhanced the stainability for HA in peri- and intercellular matrices. Immunohistochemistry with or without enzymatic pre-treatment demonstrated that immunoreactivity for CS, KS, and PC was distinctly discerned in chondrocytes and in the extracellular matrix located in the middle and deep zones. In particular, the immunoreactivity for KS and PC was augmented by pre-treatment with chondroitinase ABC not only in chondrocytes but in the extracellular matrix located in the middle and deep zones. Microbiochemical analysis corresponded well with histochemical and immunohistochemical results. These results suggest that HA is abundantly synthesized and secreted in chondrocytes located in the transition between the superficial and middle zones. 相似文献
145.
Herpes virus was reacted with an early rabbit antiserum containing predominantly complement-requiring neutralizing (CRN) antibody to produce CRN-antibody-sensitized virus (SV), and the action of complement (C') upon SV was studied. Reduction of infectivity due to C' was about equal with undiluted and 1000-fold diluted SV. Even higher dilutions which contained about 10 to 100 infectious units per 0.05 ml were also completely inactivated by C'. Kinetic experiments revealed that the velocity of titer reduction in the presence of C' of 100-fold diluted SV was not slower than that of undiluted SV. When SV was first treated with C' and then diluted 100-fold, the surviving virus showed but a slightly reduced efficiency of filtration through the 0.45 μ Millipore membrane as compared with SV first diluted 1: 100 and then treated with C'. The titer reduction of SV–C'1 complexes in the presence of C'4 followed a one-hit curve. These results indicated that the reduction of infectivity of SV due to C' was not a result of immunoaggregation of infectious SV. Alternative possible mechanisms of the action of C' are discussed. 相似文献
146.
Taxonomic relationships betweenCalamagrostis langsdorffii andC. sachalinensis were investigated in connection with the discovery that a considerable number of plants intermediate between these species
occur in the high mountains of central Honshu. The studies were mainly carried out on the basis of cytological voucher specimens
for which a chromosome number was known. Their morphological features, leaf flavonoids and pollen grains were subjected to
examination. Hybridization, polyploidy and gametophytic apomixis are related to the organization of this complex, and plants
referable to the “intermediates” are apomictic octoploids superimposed on the ancestral and sexual tetraploid taxa. The internal
structure of this complex is discussed together with its probable course of evolution, and a diagram showing the outline of
the structure is presented. The variability of morphological features and flavonoid patterns of the plants concerned is examined,
and the delimitation ofC. langsdorffii andC. sachalinensis is made clearer. 相似文献
147.
Landscape and Ecological Engineering - The COVID-19 pandemic has significantly reduced human activities through city lock-downs and restricted traffic flows. It had been suggested that roadkill... 相似文献
148.