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11.
Tairo Oshima 《Biochemical and biophysical research communications》1975,63(4):1093-1098
A new polyamine compound was isolated from an extreme thermophile, HB8, and named thermine. Chemical structure of thermine was determined to be 1,11-diamino-4,8-diazaundecane, NH2CH2CH2CH2NHCH2CH2CH2NHCH2CH2CH2NH2. 相似文献
12.
Tairo Oshima 《Plant Physiology and Biochemistry》2010,48(7):521-526
Thermus thermophilus, an extreme thermophile belonging to Domain Bacteria, produces unusual polyamines in addition to standard polyamines. To understand mechanisms of changes of polyamine compositions of the thermophile upon change of growth conditions such as environmental temperature, metabolic pathways of polyamine biosyntheses of T. thermophilus have been studied and a new polyamine metabolic pathway was proposed. However, many enigmas remain to be solved in future studies. In this paper, biosyntheses of two non-standard polyamines, thermospermine and sym-homospermidine which are also produced and play important roles in plant cells, of the extreme thermophile are discussed in relation to the biosynthetic reactions in plants. 相似文献
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14.
We purified a geranylgeranylglyceryl phosphate (GGGP) synthase from Thermoplasma acidophilum by several steps of chromatography. Based on the proteinase-fragment-mass-pattern analysis of the SDS-PAGE band of the partially purified protein, the DNA sequence encoding the protein was identified from the whole genome sequence database of the species. The gene encoding GGGP synthase in T. acidophilum was cloned after PCR amplification of the gene from the genomic DNA. The recombinant enzyme was expressed in Escherichia coli and purified. A single band with a molecular mass of 27 kDa was obtained by SDS-PAGE analysis. The apparent native molecular mass of the enzyme was about 50 kDa based on gel filtration chromatography, suggesting that the enzyme is active as a homodimer. As the GGGP synthase from Methanobacterium thermoautotrophicum has been reported as a pentamer, the enzymes of the two organisms have different oligomeric structures. Other characteristics, including substrate specificity, are similar for the GGGPs of these organisms. 相似文献
15.
Kawashima E Itoh D Kamaike K Terui Y Oshima T 《Nucleosides, nucleotides & nucleic acids》2003,22(5-8):1309-1311
An efficient synthesis of adenosine bearing pyrrolepolyamide 1 was achieved by coupling of 3 with 2. The CD spectra obtained at several [ligand ]/[duplex] ratios allowed verification of the formation complex of the DNA duplex [d(CGCAAATTGGC)/d(GCCAATTTGCG)] with 1. 相似文献
16.
Conversion of glucose to pyruvate via reactions homologous to the non-phosphorylated Entner-Doudoroff (non-P ED) pathway could be achieved in the presence of two amino acid catalysts, cysteine and histidine: cystine oxidizes glucose to gluconic acid by the reaction homologous to glucose dehydrogenase and histidine changes gluconic acid to 2-keto-3-deoxy gluconic acid, then to pyruvate by the reaction homologous to gluconic acid dehydratase and 2-keto-3-deoxy gluconate aldolase, respectively. Pyruvate can be converted to acetyl CoA by the reaction with CoA, TPP and FAD in the presence of cysteine and histidine, which resembles pyruvate dehydrogenase reaction. It was found that gluconic acid dehydration alone is non-specific, in contrast to other reactions. The non-P ED pathway is used by some extreme thermophiles in bacteria and archaea, usually thought as the oldest among the contemporary organisms. This study suggests the possible contribution of amino acid to the origin of the glycolytic pathway. 相似文献
17.
Nobuko Hamasaki Shinji Shirai Masaru Niitsu Katsumi Kakinuma Tairo Oshima 《Applied microbiology》1993,59(8):2720-2722
A large amount of 2-phenylethylamine was produced in cells of alkalophilic Bacillus sp. strain YN-2000. This amine is secreted in the medium during the cell growth. The amounts of 2-phenylethylamine in both cells and medium change upon changing the pH of the medium. 相似文献
18.
Recombination-Deficient Mutants of an Extreme Thermophile, Thermus thermophilus 总被引:6,自引:1,他引:5 下载免费PDF全文
Tohru Takada Satoshi Akanuma Takashi Kotsuka Masatada Tamakoshi Akihiko Yamagishi Tairo Oshima 《Applied microbiology》1993,59(8):2737-2739
Recombination-deficient strains of the extreme thermophile Thermus thermophilus have been prepared from a leucine-isoleucine mutant strain (NM6). The availability of such recombination-deficient thermophilic bacterial strains may provide especially good hosts for work with plasmid vectors. 相似文献
19.
Purification and characterization of 3-isopropylmalate dehydrogenase from a thermoacidophilic archaebacterium Sulfolobus sp. strain 7 总被引:1,自引:0,他引:1
Emiri Yoda Yoriko Anraku Hiromi Kirino Takayoshi Wakagi Tairo Oshima 《FEMS microbiology letters》1995,131(3):243-247
Abstract 3-Isopropylmalate dehydrogenase was purified (about 2000-fold) to homogeneity for the first time from an archaebacterium, Sulfolobus sp. strain 7. The enzyme showed an apparent molecular mass of about 110 kDa by gel filtration and a single 36-kDa polypeptide band on SDS-PAGE, suggesting tri- or tetrameric structure. The p I value was 6.9. The N-terminal amino acid sequence was similar to enzymes from other sources. The enzyme activity was greatly stimulated by the presence of Mn2+ , Cd2+ , Mg2+ , or Co2+ . In contrast to 3-isopropylmalate dehydrogenase from other sources, monovalent cations such as K2+ and Na2+ were neither essential for activity nor stability of the protein. The enzyme was extraordinarily thermostable. 相似文献
20.
An acido-thermophillic archaebacteria,Thermoplasma strain KO-2, produced poly(A) containing RNA. The isolated poly(A)RNA showed the messenger activity in a cell-free extract of rabbit reticulocyte, indicating that the RNA is mRNA of the archaebacteria. 7-Methylgluanosine 5-phosphate did not inhibit the reaction, suggesting that the cap structure is not present in the messenger. These results may suggest that poly(A) containing messenger arised at very early stage of evolution prior to the divergence between archaebacteria and eukaryotes. 相似文献