Production of bioflavor by regeneration from protoplasts of Ulva pertusa (Ulvales,Chlorophyta)

Protoplasts were isolated from thalli of Ulva pertusa using a mixed enzyme solution of 2.0% Cellulase Onozuka R-10, 2.0% Macerozyme R-10, and 2.0% Driselase. Isolated protoplasts regenerated cell walls, developed into thalli, and propagated in large numbers under aeration in the preparative scale-culture system. Typical bioflavor compounds produced from the regenerated plants, as well as from field-collected plants, were found to be long chain aldehydes, which gave a typical seaweed odor. The long chain aldehydes were formed enzymatically from unsaturated fatty acids and released into the culture fluid. A Percoll/mannitol discontinuous density gradient separation of the heterogeneous protoplasts led to a selection of cell lines with high production of bioflavor. The cells that regenerated from protoplasts were immobilized by polymer matrices such as alginate, -carrageenan, agarose, and agar. Living cells entrapped in alginate beads in aerated cultures survived best. However, the beads started to breakdown after two months. The immobilized cells demonstrated a higher bioflavor production than did the cultured cells.     

Sequence analysis of three plasmids harboured in Rhodococcus erythropolis strain PR4

Rhodococcus erythropolis strain PR4 has been isolated as an alkane-degrading bacterium. The strain harbours one linear plasmid, pREL1 (271 577 bp) and two circular plasmids, pREC1 (104 014 bp) and pREC2 (3637 bp), all with some sequence similarities to other Rhodococcus plasmids. For pREL1, pREC1 and pREC2, 298, 102 and 3 open reading frames, respectively, were predicted. Linear plasmid pREL1 has several regions homologous to plasmid pBD2 found in R. erythropolis BD2. Sequence analysis of pREL1 and pBD2 identified common metal-resistance genes on both, but pREL1 also encodes alkane-degradation genes not found on pBD2, with enzyme constituents some of which are quite different from those of other organisms. The alkane hydroxylase consisted of a cytochrome P450 monooxygenase, a 2Fe-2S ferredoxin, and a ferredoxin reductase. The ferredoxin reductase amino acid sequence resembles the AlkT (rubredoxin reductase) sequence. A zinc-containing alcohol dehydrogenase further oxydizes alkanols, alkane oxidation products catalysed by alkane hydroxylase. Of the circular plasmids, the pREC1 sequence is partially similar to the sequence of pREAT701, the virulence plasmid found in Rhodococcus equi. pREC1 has no pREAT701 virulence genes and encodes genes for beta-oxidation of fatty acids. Thus, joint actions of enzymes encoded by pREL1 and pREC1 may enable efficient mineralization of alkanes.     

CO2 emissions from the Chinese cement sector: Analysis from both the supply and demand sides

China is the largest producer and consumer of cement worldwide, and cement production entails the release of substantial carbon dioxide (CO₂) emissions. As the cement sector is a crucial sector of the Chinese economy, understanding the role of supply‐ and demand‐side factors may help accelerate efforts to mitigate CO₂ emissions. However, few studies have analyzed the critical factors affecting CO₂ emissions in the sector based on a combined supply‐ and demand‐side perspective. In this study, we developed an integrated framework that included eleven indicators covering both the supply and demand sides. Results revealed that improving cement production technology cannot offset CO₂ emissions from the growth in demand for cement. Improving technology on the supply side would considerably reduce CO₂ emissions from Chinese cement production; nevertheless, the combination of rapid urbanization, GDP growth, and an ultra‐high fixed capital formation ratio on the demand side increased CO₂ emissions nearly 25‐fold from 1990 to 2015. Notably, some demand‐side factors also had an effect that reduced CO₂ emissions. The in‐use stock per unit of fixed capital formation and output per in‐use stock reduced CO₂ emissions by 332 million metric tons, which is comparable to the contribution of technological progress. Based on these results, we examine why these demand‐side factors substantially influence CO₂ emissions in the Chinese cement sector, and we provide recommendations for policy‐makers on carbon‐reduction measures in this CO₂‐intensive sector.     

Can ground-penetrating radar detect adjacent roots and rock fragments in forest soil?

Plant and Soil - Root hemiparasite infection is considered a potential biotic stress that affects the growth of cool-season grasses, and the damage caused by a hemiparasitic plant to host grasses...     

Impact of PSCA Variation on Gastric Ulcer Susceptibility

Peptic ulcer is one of the most common gastrointestinal disorders with complex etiology. Recently we conducted the genome wide association study for duodenal ulcer and identified disease susceptibility variations at two genetic loci corresponding to the Prostate stem cell antigen (PSCA) gene and the ABO blood group (ABO) gene. Here we investigated the association of these variations with gastric ulcer in two Japanese case-control sample sets, a total of 4,291 gastric ulcer cases and 22,665 controls. As a result, a C-allele of rs2294008 at PSCA increased the risk of gastric ulcer with odds ratio (OR) of 1.13 (P value of 5.85×10⁻⁷) in an additive model. On the other hand, SNP rs505922 on ABO exhibited inconsistent result between two cohorts. Our finding implies presence of the common genetic variant in the pathogenesis of gastric and duodenal ulcers.     

Quality Evaluation of Steel,Aluminum, and Road Material Recycled from End‐of‐Life Urban Buildings in Japan in Terms of Total Material Requirement

In this study we introduce the concept of total material requirement (TMR) to quantify the quality of materials from end‐of‐life buildings. The TMRs for the recycling of materials (urban ore TMR [UO‐TMR]) from four types of Japanese buildings ( Japanese traditional wooden structure [ JTWS], wooden frame with walls structure [ WFS ], reinforced‐concrete structure [RCS], and steel‐based structure [SS]) have been estimated and the trade‐off between the increase in function of recycled materials such as steel made from scrap and the additional inputs of energy and materials required to create the increase in function were evaluated. Steel made from scrap, aluminum made from scrap, and road material are assumed to be recycled from steel products, aluminum products, and aggregate and cement concrete in the buildings, respectively. Case study analyses were carried out to determine the effect of recycling only aboveground materials compared to recycling both aboveground and subsurface materials. Also, the effect of varying the recycling rate of wooden demolition debris is determined. The UO‐TMRs of steel made from scrap range from 4.7 kilograms per kilogram (kg/kg) to 18.2 kg/kg. Urban tailings (unrecycled components) account for the greatest proportion of the UO‐TMR of steel made from scrap, and the next largest contributor is the recycling process. In the case of aluminum made from scrap, the UO‐TMRs range from 22 to 196 kg/kg, with the contribution of urban tailings generally dominant, and the second largest contributor being on‐site demolition and shredding. The UO‐TMRs of recycled road material range from 1.04 to 1.16 kg/kg and are similar for different recycling cases and types of buildings.     

Dioctophyme renale (Nematoda: Dioctophymatoidea) in the abdominal cavity of Rattus norvegicus in Japan

We collected 24 brown rats, Rattus norvegicus, in Kanagawa Prefecture in Japan and found one rat harboring a dioctophymatid nematode. A single male and a female worm were recovered from the abdominal cavity and were identified as Dioctophyme renale based on morphologic features and a BLAST DNA sequence analysis. We describe the morphological features of the adult worms and eggs from this extremely rare case of D. renale infection in a brown rat.     

Cytoplasmic assembly and selective nuclear import of Arabidopsis Argonaute4/siRNA complexes

In plants, DNA methylation can be mediated by a class of Argonaute4 (AGO4)-associated heterochromatic siRNAs (hc-siRNAs), through a pathway termed RNA-directed DNA methylation (RdDM). It has been thought that RdDM is solely a nuclear process, as both the biogenesis and functioning of hc-siRNAs take place in the nucleus. In this study, we unexpectedly found that hc-siRNAs are predominantly present in the cytoplasm. We demonstrated that AGO4 is loaded with hc-siRNAs in the cytoplasm and the formation of mature AGO4/siRNA complexes requires HSP90 and the cleavage activity of AGO4. Intriguingly, siRNA binding facilitates the redistribution of AGO4 into the nucleus, likely through inducing conformational change that leads to the exposure of the nuclear localization signal (NLS). Our findings reveal an unsuspected cytoplasmic step in the RdDM pathway. We propose that selective nuclear import of mature AGO4/siRNA complexes is a key regulatory point prior to the effector stage of RdDM.     

Cerebral-evoked responses elicited by direct stimulation of the lateral spinothalamic tract in the human

The authors recorded cerebral-evoked responses elicited by direct stimulation of the human lateral spinothalamic tract (LST) during percutaneous cordotomy to investigate central conduction of noxious stimuli. These responses consisted of four negative potentials, peak latency being 3.8 (N1), 8.4 (N2), 12.2 (N3) and 21.9 (N4) ms respectively. N1 showed wide distribution over the scalp and was considered to be of subcortical origin. N2-N4 were distributed in both the temporal and central area. The different distribution pattern of N2-N4 from conventional somatosensory-evoked potential suggested a different projection of LST from the medial lemniscus system.