全文获取类型
收费全文 | 43113篇 |
免费 | 3871篇 |
国内免费 | 33篇 |
专业分类
47017篇 |
出版年
2023年 | 166篇 |
2022年 | 309篇 |
2021年 | 599篇 |
2020年 | 395篇 |
2019年 | 489篇 |
2018年 | 641篇 |
2017年 | 555篇 |
2016年 | 1045篇 |
2015年 | 1758篇 |
2014年 | 1881篇 |
2013年 | 2459篇 |
2012年 | 2981篇 |
2011年 | 3058篇 |
2010年 | 1945篇 |
2009年 | 1657篇 |
2008年 | 2517篇 |
2007年 | 2508篇 |
2006年 | 2273篇 |
2005年 | 2338篇 |
2004年 | 2292篇 |
2003年 | 2182篇 |
2002年 | 2130篇 |
2001年 | 505篇 |
2000年 | 406篇 |
1999年 | 510篇 |
1998年 | 606篇 |
1997年 | 462篇 |
1996年 | 411篇 |
1995年 | 393篇 |
1994年 | 355篇 |
1993年 | 345篇 |
1992年 | 385篇 |
1991年 | 327篇 |
1990年 | 270篇 |
1989年 | 272篇 |
1988年 | 274篇 |
1987年 | 251篇 |
1986年 | 234篇 |
1985年 | 302篇 |
1984年 | 321篇 |
1983年 | 257篇 |
1982年 | 351篇 |
1981年 | 275篇 |
1980年 | 255篇 |
1979年 | 190篇 |
1978年 | 222篇 |
1977年 | 208篇 |
1976年 | 178篇 |
1974年 | 188篇 |
1973年 | 194篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Role of RAD51C and XRCC3 in genetic recombination and DNA repair 总被引:1,自引:0,他引:1
In germ line cells, recombination is required for gene reassortment and proper chromosome segregation at meiosis, whereas in somatic cells it provides an important mechanism for the repair of DNA double-strand breaks. Five proteins (RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3) that share homology with RAD51 recombinase and are known as the RAD51 paralogs are important for recombinational repair, as paralog-defective cell lines exhibit spontaneous chromosomal aberrations, defective DNA repair, and reduced gene targeting. The paralogs form two distinct protein complexes, RAD51B-RAD51C-RAD51D-XRCC2 and RAD51C-XRCC3, but their precise cellular roles remain unknown. Here, we show that, like MLH1, RAD51C localized to mouse meiotic chromosomes at pachytene/diplotene. Using immunoprecipitation and gel filtration analyses, we found that Holliday junction resolvase activity associated tightly and co-eluted with the 80-kDa RAD51C-XRCC3 complex. Taken together, these data indicate that the RAD51C-XRCC3-associated Holliday junction resolvase complex associates with crossovers and may play an essential role in the resolution of recombination intermediates prior to chromosome segregation. 相似文献
992.
993.
994.
Greater bone formation of Y2 knockout mice is associated with increased osteoprogenitor numbers and altered Y1 receptor expression 总被引:4,自引:0,他引:4
995.
Cytidine triphosphate synthetase (CTPS) catalyzes the rate-limiting step in the de novo synthesis of CTP, and both the yeast and human enzymes have been reported to be regulated by protein kinase A or protein kinase C phosphorylation. Here, we provide evidence that stimulation or inhibition of protein kinase A and protein kinase C does not alter the phosphorylation of endogenous human CTPS1 in human embryonic kidney 293 cells under the conditions tested. Unexpectedly, we found that low serum conditions increased phosphorylation of endogenous CTPS1 and this phosphorylation was inhibited by the glycogen synthase kinase 3 (GSK3) inhibitor indirubin-3'-monoxime and GSK3beta short interfering RNAs, demonstrating the involvement of GSK3 in phosphorylation of endogenous human CTPS1. Separating tryptic peptides from [(32)P]orthophosphate-labeled cells and analyzing the phosphopeptides by mass spectrometry identified Ser-574 and Ser-575 as phosphorylated residues. Mutation of Ser-571 demonstrated that Ser-571 was the major site phosphorylated by GSK3 in intact human embryonic kidney 293 cells by GSK3 in vitro. Furthermore, mutation of Ser-575 prevented the phosphorylation of Ser-571, suggesting that phosphorylation of Ser-575 was necessary for priming the GSK3 phosphorylation of Ser-571. Low serum was found to decrease CTPS1 activity, and incubation with the GSK3 inhibitor indirubin-3'-monoxime protected against this decrease in activity. Incubation with an alkaline phosphatase increased CTPS1 activity in a time-dependent manner, demonstrating that phosphorylation inhibits CTPS1 activity. This is the first study to investigate the phosphorylation and regulation of human CTPS1 in human cells and suggests that GSK3 is a novel regulator of CTPS activity. 相似文献
996.
997.
Melov S Adlard PA Morten K Johnson F Golden TR Hinerfeld D Schilling B Mavros C Masters CL Volitakis I Li QX Laughton K Hubbard A Cherny RA Gibson B Bush AI 《PloS one》2007,2(6):e536
Age-related neurodegenerative disease has been mechanistically linked with mitochondrial dysfunction via damage from reactive oxygen species produced within the cell. We determined whether increased mitochondrial oxidative stress could modulate or regulate two of the key neurochemical hallmarks of Alzheimer's disease (AD): tau phosphorylation, and beta-amyloid deposition. Mice lacking superoxide dismutase 2 (SOD2) die within the first week of life, and develop a complex heterogeneous phenotype arising from mitochondrial dysfunction and oxidative stress. Treatment of these mice with catalytic antioxidants increases their lifespan and rescues the peripheral phenotypes, while uncovering central nervous system pathology. We examined sod2 null mice differentially treated with high and low doses of a catalytic antioxidant and observed striking elevations in the levels of tau phosphorylation (at Ser-396 and other phospho-epitopes of tau) in the low-dose antioxidant treated mice at AD-associated residues. This hyperphosphorylation of tau was prevented with an increased dose of the antioxidant, previously reported to be sufficient to prevent neuropathology. We then genetically combined a well-characterized mouse model of AD (Tg2576) with heterozygous sod2 knockout mice to study the interactions between mitochondrial oxidative stress and cerebral Ass load. We found that mitochondrial SOD2 deficiency exacerbates amyloid burden and significantly reduces metal levels in the brain, while increasing levels of Ser-396 phosphorylated tau. These findings mechanistically link mitochondrial oxidative stress with the pathological features of AD. 相似文献
998.
Alexander Pazur Christine Schimek Paul Galland 《Central European Journal of Biology》2007,2(4):597-659
The ability to respond to magnetic fields is ubiquitous among the five kingdoms of organisms. Apart from the mechanisms that
are at work in bacterial magnetotaxis, none of the innumerable magnetobiological effects are as yet completely understood
in terms of their underlying physical principles. Physical theories on magnetoreception, which draw on classical electrodynamics
as well as on quantum electrodynamics, have greatly advanced during the past twenty years, and provide a basis for biological
experimentation. This review places major emphasis on theories, and magnetobiological effects that occur in response to weak
and moderate magnetic fields, and that are not related to magnetotaxis and magnetosomes. While knowledge relating to bacterial
magnetotaxis has advanced considerably during the past 27 years, the biology of other magnetic effects has remained largely
on a phenomenological level, a fact that is partly due to a lack of model organisms and model responses; and in great part
also to the circumstance that the biological community at large takes little notice of the field, and in particular of the
available physical theories. We review the known magnetobiological effects for bacteria, protists and fungi, and try to show
how the variegated empirical material could be approached in the framework of the available physical models. 相似文献
999.
McTiernan A Sorensen B Irwin ML Morgan A Yasui Y Rudolph RE Surawicz C Lampe JW Lampe PD Ayub K Potter JD 《Obesity (Silver Spring, Md.)》2007,15(6):1496-1512
Objectives: The effect of national exercise recommendations on adiposity is unknown and may differ by sex. We examined long‐term effects of aerobic exercise on adiposity in women and men. Research Methods and Procedures: This was a 12‐month randomized, controlled clinical trial testing exercise effect on weight and body composition in men (N = 102) and women (N = 100). Sedentary/unfit persons, 40 to 75 years old, were recruited through physician practices and media. The intervention was facility‐ and home‐based moderate‐to‐vigorous intensity aerobic activity, 60 min/d, 6 days/wk vs. controls (no intervention). Results: Exercisers exercised a mean 370 min/wk (men) and 295 min/wk (women), and seven dropped the intervention. Exercisers lost weight (women, ?1.4 vs. +0.7 kg in controls, p = 0.008; men, ?1.8 vs. ?0.1 kg in controls, p = 0.03), BMI (women, ?0.6 vs. +0.3 kg/m2 in controls, p = 0.006; men, ?0.5 kg/m2 vs. no change in controls, p = 0.03), waist circumference (women, ?1.4 vs. +2.2 cm in controls, p < 0.001; men, ?3.3 vs. ?0.4 cm in controls, p = 0.003), and total fat mass (women, ?1.9 vs. +0.2 kg in controls, p = 0.001; men, ?3.0 vs. +0.2 kg in controls, p < 0.001). Exercisers with greater increases in pedometer‐measured steps per day had greater decreases in weight, BMI, body fat, and intra‐abdominal fat (all p trend < 0.05 in both men and women). Similar trends were observed for increased minutes per day of exercise and for increases in maximal oxygen consumption. Discussion: These data support the U.S. Department of Agriculture and Institute of Medicine guidelines of 60 min/d of moderate‐to‐vigorous physical activity. 相似文献
1000.