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991.
本文用国产高分子树脂(T)接枝小牛胸腺DNA,通过亲合层析从系统性红斑狼疮SLE患者血清中纯化出抗-ds DNA抗体和抗-ss DNA抗体。酶联免疫吸附分析(ELISA)的研究表明:SLE抗-DNA抗体和DNA结合的差异性很大,是高度非均一性的。抗-ss DNA抗体不仅组成成分比抗-ds DNA抗体复杂,ss DNA/抗-ssDNA亲合能力也明显高于ds DNA/抗-ds DNA。纯化的抗-DNA抗体以IgG类抗体占主导,同时也有其它类型抗体存在(例如IgM等)。抗-ds DNA抗体有较抗-ss DNA抗体高的IgG含量(两者的IgG/IgM分别是7.0和4.0),说明IgG抗-DNA抗体更倾向于同dsDNA结合。 相似文献
992.
以台中65等基因F_1不育系为遗传测验种,测定了栽培稻(O.sativa)45个品种在3个F_1不育基因座的基因型和等位基因的分化度。在S-E3基因座上,除Dular带有S_i/S_i基因型外,其余被测品种均带有S_i/S_i基因型。在S-E2和S-E5基因座上,籼型品种带有高频率的S_i基因,而粳型品种带有高频率的S_i基因。S_i和S_i均具有不同的分化度。籼型品种携带的S_i基因和粳型品种携带的S_i基因具有较高的分化度。中间型品种和广亲和品种的等位基因分化在S-E2基因座上与粳型品种相似,而在S-E5基因座上与籼型品种相似。此外,分析了各类型品种相互杂交F_1杂种在S-E2和S-E5基因座的杂合率、杂合度和杂合性。与籼/粳杂种相比,中间型品种(包括广亲和品种)与籼型和粳型品种杂交,F_1杂种均具有较低的平均杂合性,从而表现出较高的亲和性。因此,无论是杂种不育性还是杂种亲和性均由花粉不育基因控制。花粉不育基因也称为特异亲和基因。 相似文献
993.
The conformation of native and denaturedPhaseolus coccineus var. rubronanus lectin was studied by circular dichroism (CD) and correlated to the hemagglutinating activity. The far-UV CD spectrum at 25°C showed a broad, negative band around 223 nm and a positive one at 196 nm. CD data analysis of the lectin indicated a -sheet-rich protein. At high temperatures, the spectrum was blue-shifted with increasing magnitude; these changes correlated well with the loss of the activity. The conformation of lectin betweenpH 2 and 10 remained essentially unchanged. AtpH 13 the CD spectrum resembled that of unordered form with a negative band near 200 nm and the activity was completely lost. The denatured lectin in 6 M guanidine hydrochloride would be renatured upon diluting the denaturant to 0.75 M; the changes in CD spectrum again correlated well with the loss of the activity. The effect of sodium dodecyl sulfate on the lectin was drastic; it sharply increased thea-helix at the expense of the -sheet and reduced the activity; the changes reached a plateau above 20 mM surfactant. 相似文献
994.
S Y Yan Z R Mao H Y Yang M A Tu S H Li G P Huang G S Li L Guo G Q Jin R F He 《The International journal of developmental biology》1991,35(4):429-435
The nucleus of a blastula cell from Tilapia (Oreochromis nilotica, family Cichlidae, order Perciformes) was transplanted into an enucleated egg of Loach (Paramisgurnus dabryanus, family Cobitidae, order Cypriniformes). From among 3747 nucleo-cytoplasmic hybrid (NCH) eggs two NCH larval fish (0.05%) were obtained; one died on the 6th day and the other died on the 12th day after the operation. Morphological examinations showed that both NCH larval fish had developed normally with an opened mouth except they could not take food after complete utilization of their egg yolk on the 5th day of development. The possible mechanisms for obtaining such inter-order NCH larval fish are discussed. This is the first report indicating that inter-order NCH larval fish can be obtained in spite of their evolutionary divergence. 相似文献
995.
Gao HR Shi TF Yang CX Zhang GW Zhang D Jiao RS Zhang Y Xu MY Zhu H 《Neurochemical research》2012,37(2):428-435
Dopamine regulates pain perception in some areas of the central nervous system. Previously, we have confirmed that dopamine
potentiated the electric activities of the evoked discharges of pain-excited neurons (PENs) and inhibited those of pain-inhibited
neurons (PINs) in the parafascicular nucleus (Pfn) of normal rats. The mechanism of action of dopamine on pain-related neurons
in the Pfn of morphine-dependent rat is still unknown. The present study aimed to determine the effects of dopamine and its
receptor antagonist droperidol on the pain-evoked responses of the PEN and PIN in the Pfn of morphine-dependent rats, and
to compare the effects between the morphine-dependent rat and the normal rat. The trains of electric impulses applied to the
sciatic nerve were used as noxious stimulation. The discharges of PEN or PIN in the Pfn were recorded by using a glass microelectrode.
The results showed that intra-Pfn microinjection of dopamine decreased the frequency of noxious stimulation-induced discharges
of PEN and increased the frequency of PIN. The intra-Pfn administration of droperidol produced an opposite effect. These results
demonstrated that dopamine is involved in nociceptive modulation in the morphine-dependent rat, the responses to noxious stimulation
between normal rat and morphine-dependent rat are completely opposite. The effect of dopamine is through the dopamine D2 receptor of PENs and PINs in Pfn. The results suggest that the dopamine system of the Pfn may become a therapeutic target
for analgesia and the treatment of morphine dependence. 相似文献
996.
It has been known that Rho-associated protein kinase (ROCK) signaling regulates the migration of vascular smooth muscle cells (VSMCs). However, the isoform-specific roles of ROCK and its underlying mechanism in VSMC migration are not well understood. The current study thus aimed to investigate the roles of ROCK1/2 and their relationship to the MAPK signaling pathway in platelet-derived growth factor (PDGF)-induced rat aorta VSMC migration by manipulating ROCK gene expression. The results revealed that ROCK1 small interfering ribonucleic acid (siRNA) rather than ROCK2 siRNA decreased PDGF-BB-generated VSMC migration, and upregulation of ROCK1 expression via transfection of constructed pEGFP-C1/ROCK1 plasmid further increased the migration of PDGF-BB-treated VSMCs. In PDGF-treated VSMCs, ROCK1 siRNA did not affect the phosphorylation levels of ERK and p38 in the cytoplasm, but decreased the level of ERK phosphorylation in the nucleus. These findings demonstrate that activated ROCK1 can promote VSMC migration through facilitating phosphorylation and nuclear translocation of ERK protein. 相似文献
997.
The enzyme alanine-glyoxylate aminotransferase 1 (AGT) functions to detoxify glyoxylate before it is converted into harmful oxalate. In mammals, mitochondrial targeting of AGT in carnivorous species versus peroxisomal targeting in herbivores is controlled by two signal peptides that correspond to these respective organelles. Differential expression of the mitochondrial targeting sequence (MTS) is considered an adaptation to diet-specific subcellular localization of glyoxylate precursors. Bats are an excellent group in which to study adaptive changes in dietary enzymes; they show unparalleled mammalian dietary diversification as well as independent origins of carnivory, frugivory, and nectarivory. We studied the AGT gene in bats and other mammals with diverse diets and found that the MTS has been lost in unrelated lineages of frugivorous bats. Conversely, species exhibiting piscivory, carnivory, insectivory, and sanguinivory possessed intact MTSs. Detected positive selection in the AGT of ancestral fruit bats further supports adaptations related to evolutionary changes in diet. 相似文献
998.
Jiandong Zheng Xixiang Huo Yang Huai Lin Xiao Hui Jiang John Klena Carolyn M. Greene Xuesen Xing Jigui Huang Shali Liu Youxing Peng Hui Yang Jun Luo Zhibin Peng Linlin Liu Maoyi Chen Hui Chen Yuzhi Zhang Danqin Huang Xuhua Guan Luzhao Feng Faxian Zhan Dale J. Hu Jay K. Varma Hongjie Yu 《PloS one》2016,11(3)
999.
1000.
为制备用地高辛精(Digoxigenin,Dig)标记的酶氨酸羟化酶(TyrosineHydroxylase,TH)RNA探针,本研究用分子生物学技术重组质粒PGEMTHI,即分别将携带T7和Sp6启动子的PGEM-3zf质粒和携带TH基因的PKSTH质粒用限制性内切酶消化并行分离纯化,得到带T7和sp6启动子的DNA片段和TH基因片段;经T4DNA连接酶连接后转入大肠杆菌,得到pGEMTH1重组克隆。经小量提取质粒井用酶切分析检测,证实其确有TH基因且方向正确。将此质粒再经限制性内切酶消化则得到线状DNA片段,用T7RNA聚合酶转录合成带有Dig标记的高比活度的单链RNA探针;经斑点杂交试验证实该探针具有较高的可靠性。这将为基因治疗帕金森氏病动物模型的疗效检测提供有效手段。 相似文献