The required for
Mla12 resistance (RAR1) protein is essential for the plant immune response. In rice, a model monocot species, the function of
Oryza sativa RAR1 (OsRAR1) has been little explored. In our current study, we characterized the response of a rice
osrar1 T-DNA insertion mutant to infection by
Magnaporthe oryzae, the causal agent of rice blast disease.
osrar1 mutants displayed reduced resistance compared with wild type rice when inoculated with the normally virulent
M. oryzae isolate PO6-6, indicating that
OsRAR1 is required for an immune response to this pathogen. We also investigated the function of
OsRAR1 in the resistance mechanism mediated by the immune receptor genes
Pib and
Pi5 that encode nucleotide binding-leucine rich repeat (NB-LRR) proteins. We inoculated progeny from
Pib/osrar1 and
Pi5/osrar1 heterozygous plants with the avirulent
M. oryzae isolates, race 007 and PO6-6, respectively. We found that only
Pib-mediated resistance was compromised by the
osrar1 mutation and that the introduction of the
OsRAR1 cDNA into
Pib/osrar1 rescued
Pib-mediated resistance. These results indicate that
OsRAR1 is required for
Pib-mediated resistance but not
Pi5-mediated resistance to
M. oryzae.
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