Antimicrobial Activity of Kumazasa (Sasa albo-marginata)

The organic solvent extract of Kumazasa leaves (Sasa albo-marginata) showed antimicrobial activity against bacteria, fungi and yeast. Kumazasa at a concentration of 0.2-1.0% showed stronger antimicrobial activity than potassium sorbate or sodium benzoate at the same concentration. Both acidic and phenolic fractions of the extract showed strong antimicrobial activity. Thirty acidic and phenolic compounds were identified by GC and GC-MS analysis. Acetic, propionic, benzoic, phenylacetic, salicylic, 3-hydroxybenzoic and o-anisic acids, and guaiacol, phenol, 4-ethylphenol, xylenol and 4-vinylphenol were the main components. It was estimated that these components play an important role in the formation of the antimicrobial activity of Kumazasa extract.     

Isoflavonoid Constituents of Soybeans and Isolation of a New Acetyl Daidzin

The flavonoid constituents of defatted soybeans were studied. Two kinds of new natural isoflavones and four kinds of isoflavones were isolated by rechromatography on silica gel and Sephadex LH–20 columns of ethyl acetate extracts.

One of the new isoflavones was identified as 6″-O-acetyl daidzin, C₂₃H₂₂O₁₀, by UV, IR, PMR and CMR, and the four kinds of isoflavones were also identified as daidzein, daidzin, genistein and genistin.

High performance liquid chromatography of the ethyl acetate and acetone extracts were carried out, and six main peaks corresponding to the six isoflavones were recognized.

Glycitein and glycitein-7-O-glucoside were not detected in the soybeans.     

Changes in 1-Aminocyclopropane-1-carboxylic Acid Content and Ethylene Production in Hiproly Barley Callus during Differentiation

During differentiation after auxin withdrawal, the change in the ethylene production of Hiproly barley callus paralleled the change in 1-aminocyclopropane-1-carboxylic acid (ACC) content. The levels of ACC and ethylene production decreased rapidly, and then increased in Hiproly barley callus.

Aminooxyacetic acid (AOA) prevented the ACC and ethylene production of the callus. Moreover, aminoisobutyric acid (AIB) also inhibited the ethylene production, but did not prevent the ACC synthesis of the callus. On the other hand, methylglyoxal-bis(guanylhydrazone) (MGBG) greatly enhanced the ACC and ethylene production. Formation of adventitious roots in Hiproly barley callus was enhanced by the cultivation in the medium containing AIB or AOA. However, differentiation of the callus was strongly inhibited by MGBG.

Thus, prevention of ethylene production may be significant for differentiation of Hiproly barley callus.     

The Preparation and Geometrical Isomerism of Substituted α-Methylthio-Cinnamic Acids

α-Methylthio-cinnamic acid and its substituted analogues (III) were synthesized from their respective β-aryl-α-thiopyruvic acids (II). In connection with the study on the tautomeric ene-thiol structure of β-aryl-α-thiopyruvic acids (II), 4-arylidenerl,3-oxathiolan-5-one (IV) were prepared from compounds II.     

Phosphodiesterase in Microsomes from Bovine Milk

Phosphodiesterase was solubilized from bovine milk microsomes and partially purified. The purified enzyme showed 20-fold specific activity compared with that of microsomes, and 1,500-fold with that of the original milk.

The properties of the enzyme were investigated by using NpT. The pH optimum was at 9.5. The enzyme was inhibited with EDT A and reactivated with the addition of magnesium or calcium ions. This enzyme was strongly inhibited with reducing reagents. K_m, value was 7.4 x 10^-4 M for NpT at pH 9.5.

RNA was hydrolyzed completely to 5′-mononucleotides, and this enzyme may be considered to show the exonucleolytic action for RNA.     

Ribonuclease in Bovine Milk

The RNase (EC 2.7.7.16) in bovine milk was partially purified from milk whey. The basic properties and the hydrolyzing specificity of this enzyme were studied. This enzyme was heat stable. When RNA was used as the substrate, the pH optimum was 7.5 and 3′- nucleotides were produced, but the extent of the hydrolysis stopped at 31 per cent and core was left. C! and U! were hydrolyzed but purine cyclic nucleotides were not. Poly U was digested to 3′-uridylic acid passing through U! but poly A was not. These properties are quite similar to pancreatic RNase.     

Synthesis of 2-Acetamido-2-deoxy-1,6-dithio-d-glucose and its Derivatives

2-Acetamido-3,4-di-Oacetyl-2,6-dideoxy-6-S-acetyl-6-thio-d-glucopyranosyl chloride (III) was condensed with potassium thiolacetate, potassium ethylxanthate or thiourea to give three crystalline derivatives of 2-acetamido-2-deoxy-1,6-dithio-d-glucose. An attempt to prepare 2-acetamido-1,2,6-trideoxy-1,6-dimercapto-D-glucose (VII) from 2-acetamido-3,4-di-O-acetyl-1,2,6-trideoxy-1,6-di-S-acetyl-1,6-dithio-β-d-glucopyranose was described. 2-Acetamido-3,4-di-O-acetyl-1,2,6-trideoxy-1-mercapto-6-S-acetyl-6-thio-β-d-glucopyranose (VIII) was synthesized from the condensation product of III with thiourea.     

Isolation and Characterization of Globulin from Seeds of Amaranthus hypochondriacus L.

Seeds of grain amaranths contain a high amount (about 60% of total nitrogen) of albumin and globulin and a trace amount of prolamin. From salt-soluble extracts of A. hypochondriacus seeds, a globulin (440,000 in apparent molecular weight and ) was purified by Sepharose 6B gel and DEAE-cellulose column chromatographies. The protein comprised at least four kinds of subunits whose molecular weights were 36,000, 32,000, 20,000 and 18,000, respectively. The amino acid composition of the globulin was almost similar to those of soybean and oat globulins.