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排序方式: 共有235条查询结果,搜索用时 15 毫秒
101.
Two acid RNases were purified from bovine spleen by means of ammonium sulfate fractionation, chromatographies on-phospho-cellulose, heparin-Sepharose CL-6B, poly G-Sepharose, and 2', 5'-ADP-Sepharose, and gel filtration on Toyopearl HW 55F. Both purified preparations were homogeneous as judged by disc electrophoresis at pH 4.3. They were designated as RNase BSP1 and RNase BSP2 in the order of elution from a phospho-cellulose column. RNase BSP2 was immunologically indistinguishable from RNase K2 from bovine kidney. RNase BSP1 was a typical pyrimidine base-specific, uridylic acid-preferential RNase and had very sharp pH optimum at 6.5. RNase BSP1 thus obtained was a glycoprotein giving two major bands on SDS-slap electrophoresis. Although the apparent molecular weight of RNase BSP1 was distributed in the range of 27,000-20,000, it decreased to about 17,000-18,000 after endoglycosidase F digestion. The N-terminal amino acid sequence up to the 20th amino acid had no homology to those of RNase K2 and RNase A. 相似文献
102.
Distribution of two urinary ribonuclease-like enzymes in human organs and body fluids 总被引:4,自引:0,他引:4
In order to determine the distribution of two human urinary RNase (RNase Us and RNase UL)-like enzymes in human tissues and body fluids, enzyme immunoassay systems were established using rabbit anti-RNase sera. The sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previously reported. In the enzyme immunoassay, the cross reactivities of anti-RNase UL serum towards RNase Us, bovine kidney RNase K2, bovine RNase A, and bovine seminal RNase Vs were less than 1%. The cross reactivity of anti-RNase Us-serum towards RNase UL was less than 0.5% and cross reactivities were minimal for RNase A, RNase K2, and RNase Vs. The RNase levels in human organs and body fluids were measured by enzyme immunoassay. In milk, semen and saliva, only RNase UL-like enzyme was found. Both RNase Us- and RNase UL-like enzymes were found in kidney, stomach, and pancreas and the RNase Us/RNase UL ratios were 0.49, 1.35, and 0.34, respectively. In lung, liver, spleen, and leukocytes, most of the RNase activity was accounted for by RNase Us-like enzyme. The activity of RNase Us-like enzyme was especially high in lung, spleen, and leukocytes. The crude extracts of several tissues and body fluids were separated by phosphocellulose column chromatography and the contents of the two urinary RNase-like enzymes were determined by enzyme immunoassay. In stomach, kidney, pancreas, and serum, both enzymes were present in multiple forms. In spleen and lung, both the major RNase (RNase Us) and minor RNase (RNase UL) existed in two forms.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
103.
Tadaaki Hashimoto Tetsuji Yamada Akiko Tada Shinji Kawamata Yoshikazu Tanaka Pernpong Sriprasertsak Yuki Ichinose Hisaharu Kato Satoshi Izutsu Tomonori Shiraishi Hachiro Oku Yoshiaki Ohtsuki 《Plant cell reports》1992,11(4):183-187
Summary High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 g/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.Abbreviations CAT
chloramphenicol acetyltransferase
- PAL
phenylalanine ammonia-lyase
- CM
acetylated chloramphenicol
- GSH
reduced glutathione
- NOS
nopaline synthase
- ES
electroporation solution
- CaMV
cauliflower mosaic virus
- GUS
-glucuronidase
- CHS
chalcone synthase
- 2,4-D
2, 4-dichlorophenoxyacetic acid
Present address: Research Institute, Takasago Perfumery Inc, 5-31-36, Kamata, Minato, Tokyo, 144, Japan Toso Inc, 4560 Oaza-Tomita, Shin-nanyo, Yamaguchi, 746, Japan Central Laboratory of Green Complex, Kasetsert University, Kamphaensaen, Nakohn Pathom, Thailand 相似文献
104.
Murata Yoshiyuki; Yoshihashi Manabu; Obi Ichiro; Kakutani Tadaaki 《Plant & cell physiology》1998,39(10):1039-1044
The patch-clamp technique was used to study effect of the Ca2+on K+ channels in the plasma membrane of protoplasts isolatedfrom tobacco (Nicotiana tabacum L., cv. Bright Yellow) culturedcells in suspension. The outward rectifying whole-cell K+ currentswere not affected by in-tracellular Ca2+, but they were reducedwith increasing extracellular Ca2+. Neither extracellular norintracellular Ca2+ affected the permeability ratios (pK+/PNa+)of the plasma membrane. These results suggest that the inhibitionof outward-rectifying K+ channels by extracellular Ca2+may partiallycontribute towards the mitigation of detrimental effects ofsalinity on growth by extracellular Ca2+. (Received January 19, 1998; Accepted July 30, 1998) 相似文献
105.
In the brain, Serpinb6 was identified as an endogenous inhibitor of neuropsin, a member of the S1 (clan SA) family of serine proteases [J. Biol. Chem. 276 (2001) 14562]. In the present study, we investigated the localization of Serpinb6 in the adult mouse brain using in situ hybridization histochemistry and immunohistochemistry. Region-specific patterns of expression were observed and two characteristics were recognized. First, the forebrain limbic area that expressed neuropsin mRNA contained Serpinb6 mRNA at moderate levels but not the lateral septum. On the other hand, Serpinb6 mRNA was also expressed moderately in the substantia nigra-ventral tegmental area system, whose fibers projected to the lateral septum. Additionally, Serpinb6 protein was detected in the lateral septum. Together, it was suggested that the expression of neuropsin in the brain is regulated entirely by Serpinb6. Second, Serpinb6 mRNA and the protein were strongly expressed in most somatic and visceral motoneurons among cranial nerve nuclei. This suggests that another serine protease is regulated by Serpinb6 in motoneurons and/or fibers. 相似文献
106.
Tanenobu Harada Atsushi Iwai Tadaaki Miyazaki 《Apoptosis : an international journal on programmed cell death》2010,15(10):1247-1255
Death associated protein 3 (DAP3) is known to be a highly conserved protein, and is responsible for regulating apoptosis induced
by various stimuli. To understand the molecular mechanism of how DAP3 induces apoptosis, we performed yeast two-hybrid screening,
and identified a novel DAP3-binding protein termed death ligand signal enhancer (DELE). In this report, we show that DELE
actually binds to DAP3 in mammalian cells. We found that the cells stably expressing DELE are susceptible to apoptosis induction
by the stimulation of TNF-α and TRAIL. In addition, knockdown of DELE expression rescued the HeLa cells from apoptosis induction
by these stimuli. Moreover, activation of caspase-3, caspase-8 and caspase-9 induced by stimulation of TNF-α, anti-Fas or
TRAIL was significantly inhibited by the knockdown of DELE expression. These results demonstrated the biological significance
of DELE for apoptosis signal mediated by death receptors. 相似文献
107.
Ikeda E Hirose M Kotobuki N Shimaoka H Tadokoro M Maeda M Hayashi Y Kirita T Ohgushi H 《Biochemical and biophysical research communications》2006,342(4):1257-1262
We isolated dental papilla from impacted human molar and proliferated adherent fibroblastic cells after collagenase treatment of the papilla. The cells were negative for hematopoietic markers but positive for CD29, CD44, CD90, CD105, and CD166. When the cells were further cultured in the presence of beta-glycerophosphate, ascorbic acid, and dexamethasone for 14 days, mineralized areas together with osteogenic differentiation evidenced by high alkaline phosphatase activity and osteocalcin contents were observed. The differentiation was confirmed at both protein and gene expression levels. The cells can also be cryopreserved and, after thawing, could show in vivo bone-forming capability. These results indicate that mesenchymal type cells localize in dental papilla and that the cells can be culture expanded/utilized for bone tissue engineering. 相似文献
108.
109.
Nakagawa Y Takahashi A Kajihara A Yamakawa N Imai Y Ota I Okamoto N Mori E Noda T Furusawa Y Kirita T Ohnishi T 《Biochemical and biophysical research communications》2012,423(4):654-660
Although mutations and deletions in the p53 tumor suppressor gene lead to resistance to low linear energy transfer (LET) radiation, high-LET radiation efficiently induces cell lethality and apoptosis regardless of the p53 gene status in cancer cells. Recently, it has been suggested that the induction of p53-independent apoptosis takes place through the activation of Caspase-9 which results in the cleavage of Caspase-3 and poly (ADP-ribose) polymerase (PARP). This study was designed to examine if high-LET radiation depresses serine/threonine protein kinase B (PKB, also known as Akt) and Akt-related proteins. Human gingival cancer cells (Ca9-22 cells) harboring a mutated p53 (mp53) gene were irradiated with 2 Gy of X-rays or Fe-ion beams. The cellular contents of Akt-related proteins participating in cell survival signaling were analyzed with Western Blotting 1, 2, 3 and 6h after irradiation. Cell cycle distributions after irradiation were assayed with flow cytometric analysis. Akt-related protein levels decreased when cells were irradiated with high-LET radiation. High-LET radiation increased G(2)/M phase arrests and suppressed the progression of the cell cycle much more efficiently when compared to low-LET radiation. These results suggest that high-LET radiation enhances apoptosis through the activation of Caspase-3 and Caspase-9, and suppresses cell growth by suppressing Akt-related signaling, even in mp53 bearing cancer cells. 相似文献
110.
Shigeki Nanjo Tadaaki Yamada Hiroshi Nishihara Shinji Takeuchi Takako Sano Takayuki Nakagawa Daisuke Ishikawa Lu Zhao Hiromichi Ebi Kazuo Yasumoto Kunio Matsumoto Seiji Yano 《PloS one》2013,8(12)