全文获取类型
收费全文 | 161篇 |
免费 | 4篇 |
出版年
2022年 | 1篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 4篇 |
2013年 | 26篇 |
2012年 | 6篇 |
2011年 | 10篇 |
2010年 | 4篇 |
2009年 | 1篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 7篇 |
2005年 | 11篇 |
2004年 | 12篇 |
2003年 | 10篇 |
2002年 | 4篇 |
2001年 | 1篇 |
1999年 | 3篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 6篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1976年 | 2篇 |
排序方式: 共有165条查询结果,搜索用时 15 毫秒
81.
Isao Takeda Tadaaki Ooka Shiro Hayakawa 《Bioscience, biotechnology, and biochemistry》2013,77(7):703-705
Adenine-auxotrophic mutant of Bacillus licheniformis formed considerable amount of guanosine from guanine. The guanosine formation was stimulated by the addition of penicillin to the growing cells and by the presence of uridine in the crude extract. The crude extract preserved for long time showed the changes of the enzyme actions for added guanine. 相似文献
82.
Koichi Hayashi Tadaaki Matsuda Toru Takeyama Tetsuo Hino 《Bioscience, biotechnology, and biochemistry》2013,77(4):378-384
The solbilities of l-basic amino acids in the type of free, monohydro-chloride and dihydrochloride in water were determined, and the results were formulated as follows.l-Arginine: log S = 0.9770+0.01345t (t is from 0°~70°C)l-Histidine: log S = 0.3627+0.00905t (t is from 0°~70°C)l-Arginine monohydrochloride: log S = 1.6532+0.01301t (t is from 0°~70°C)l-Lysine monohydrochloride dihydrate: log S = 1.6990+0.01294t (t is from 0°~55°C)l-Lysine monohydrochloride monohydrate: log S = 1.7404+0.01256t (t is from 55°~70°C)l-Lysine dihydrochloride: log S = 2.2138+0.00409t (t is from 0°~70°C)l-Histidine dihydrochloride: log S = 1.9085+0.00265t (t is from 0°~70°C)Three component systems (basic amino acid, hydrochloric acid, water) were studied and the solubilities in mixed solution system of alcohol-water were also investigated. 相似文献
83.
Tomohiro Hosoya Fumihiko Sakai Maya Yamashita Takuya Shiozaki Tsutomu Endo Ken Ukibe Hiroshi Uenishi Yukio Kadooka Tomohiro Moriya Hisako Nakagawa Yosuke Nakayama Tadaaki Miyazaki 《PloS one》2014,9(9)
Lactobacillus helveticus SBT2171 (LH2171) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacture of cheese. We recently reported that consumption of cheese manufactured using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in mice. In this study, we have examined whether LH2171 itself exerts an inhibitory effect on the excessive proliferation of lymphocytes. We found that LH2171 inhibited the proliferation of LPS-stimulated mouse T and B cells, and the human lymphoma cell lines, Jurkat and BJAB. Cell cycle analysis showed an accumulation of LH2171-treated BJAB cells in the G2/M phase. Further, phosphorylation of c-Jun N-terminal kinase (JNK) and c-Jun was reduced by LH2171 in BJAB cells. Subsequently, expression of cell division cycle 2 (CDC2), regulated by the JNK signaling pathway and essential for G2/M phase progression, was inhibited by LH2171. It was also demonstrated that intraperitoneal administration of LH2171 strongly alleviated symptoms of collagen-induced arthritis (CIA) in mice. These findings suggest that LH2171 inhibits the proliferation of lymphocytes through a suppression of the JNK signaling pathway and exerts an immunosuppressive effect in vivo. 相似文献
84.
85.
Tomoaki Koshizawa Toshiharu Morimoto Gen Watanabe Toshiaki Watanabe Nao Yamasaki Yoshikazu Sawada Tomoaki Fukuda Ayumu Okuda Kimiyuki Shibuya Tadaaki Ohgiya 《Bioorganic & medicinal chemistry letters》2017,27(15):3249-3253
We describe the discovery and optimization of a novel series of furo[3,2-d]pyrimidines as G protein-coupled receptor 119 agonists. Agonistic activity of 4 (EC50 = 129 nM) was improved by replacing the intramolecular hydrogen bond between the fluorine atom and the aniline hydrogen in the head moiety with a covalent C-C bond to enhance conformational restriction, which consequently gave a lead compound 12 (EC50 = 53 nM). Optimized compound 26, which was identified by the further optimization of 12, exhibited potent activity (EC50 = 42 nM) with improved clearance in liver microsomes and induced a 33% reduction in blood glucose area under the curve at a dose of 10 mg/kg in an oral glucose tolerance test in C57BL/6N mice. 相似文献
86.
Enya Y Nagata S Masutomi Y Kitagawa H Takagaki K Oka N Wada T Ohgi T Yano J 《Bioorganic & medicinal chemistry》2008,16(20):9154-9160
We have synthesized diastereomerically pure diadenosine 3',5'-boranophosphates (Ap(b)A) by using the boranophosphotriester method from ribonucleosides protected with the 2'-hydroxy protecting group 2-cyanoethoxymethyl (CEM). Melting curves of the triple-helical complex of the dimer Ap(b)A and 2poly(U) at high ionic strength revealed that presumptive (Sp)-Ap(b)A had a much higher affinity and presumptive (Rp)-Ap(b)A a much lower affinity for poly(U) than the natural dimer ApA did. In contrast, the affinities of these dimers for poly(dT) were similar. Both the (Rp)- and the (Sp)-boranophosphate diastereomers showed much higher resistance to digestion by snake venom phosphodiesterase and nuclease P1 than ApA did. They have potential for use as synthons to be incorporated into boranophosphate oligonucleotides. In particular, because oligonucleotides containing Sp boranophosphate nucleotides are expected to bind more strongly and specifically to RNA than natural oligoribonucleotides do, they may find application in the isolation and detection of functional RNA in basic research and diagnostics. 相似文献
87.
88.
89.
Titanium has been utilized in the field of orthopaedic and dental reconstructive surgery, but mineralization through osteogenic differentiation of osteogenic cells on titanium surfaces has not been fully investigated. Here we cultured rat mesenchymal stem cells (MSCs) on the surfaces of titanium dishes in osteogenic media containing calcein which is a calcium-binding fluorescence dye. On titanium dishes, MSCs showed high viability to adhere to the surfaces and excellent proliferation. At day 14 of culture, MSCs differentiated into osteoblasts to form mineralized matrices on titanium dishes as well as tissue culture polystyrene (TCPS) dishes which are widely recognized as optimal culture substrates. Calcein was incorporated into the bone minerals fabricated by MSCs cultured on both substrates to show green emission under fluorescence microscopy. The fluorescence intensity was quantified with an image analyser during culture periods. These results indicate that the surfaces of titanium showed a high adhesion/proliferation potential to MSCs and that the titanium effectively supported the osteogenic differentiation of MSCs comparable to TCPS dishes. Therefore, the titanium is an effective scaffold that is applicable in bone reconstruction surgery. 相似文献
90.