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993.
Okumura-Noji K Sasai K Zhan R Kawaguchi H Maruyama H Tada T Takahashi H Okazaki M Miida T Sakuma N Kimura G Ohta N Yokoyama S 《Biochemical and biophysical research communications》2001,281(2):305-310
It was investigated whether proteasome activity was implicated in susceptibility of human vascular smooth muscle cells (VSMCs) to Fas-mediated death. Human fetal aorta smooth muscle cells were treated with agonistic anti-Fas antibody (CH11) and proteasome inhibitors (MG115 or MG132) and then cell death was determined by morphology, viability, and DNA fragmentation. The present study reports that: (a) crosslinking of Fas receptor with anti-Fas antibody in the presence of proteasome inhibitor-induced death and DNA degradation in human VSMCs that were blocked by caspases inhibitor z-DEVD.fmk; (b) cotreatment with anti-Fas antibody and proteasome inhibitor activated caspase-3; (c) proteasome inhibitors did not influence expression of procaspase-8, procaspase-3, c-FLIP, and Bcl-2; and (d) proteasome inhibitors up-regulated Fas and FADD. The data indicate that proteasome activity is important in survival of VSMCs and provide the first evidence that proteasome is involved in Fas signal transduction. The present study proposes novel mechanism(s) by which VSMCs become susceptible to FasL. 相似文献
994.
T-targets: clues to understanding the functions of T-box proteins 总被引:10,自引:0,他引:10
995.
Shimizu A Tada K Shukunami C Hiraki Y Kurokawa T Magane N Kurokawa-Seo M 《The Journal of biological chemistry》2001,276(14):11031-11040
996.
Xwnt11 and the regulation of gastrulation in Xenopus 总被引:2,自引:0,他引:2
997.
998.
Yuasa K Tada K Harita G Fujimoto T Tsukayama M Tsuji A 《Bioscience, biotechnology, and biochemistry》2012,76(3):598-600
Although some polymethoxyflavones possess several important biological properties, including neuroprotective, anticancer, and anti-inflammatory ones, sudachitin, a polymethoxyflavone from Citrus sudachi, has been little studied. In this study, we found that sudachitin inhibited nitric oxide production by suppressing the expression of inducible nitric oxide synthase in lipopolysaccharide-stimulated macrophages, indicating that sudachitin has an anti-inflammatory effect. 相似文献
999.
Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake, Lake Biwa 总被引:6,自引:0,他引:6
Kojima H Tsutsumi M Ishikawa K Iwata T Mußmann M Fukui M 《Systematic and applied microbiology》2012,35(4):233-238
Methane oxidation coupled to denitrification is mediated by 'Candidatus Methylomirabilis oxyfera', which belongs to the candidate phylum NC10. The distribution of putative denitrifying methane-oxidizing bacteria related to "M. oxyfera" was investigated in a freshwater lake, Lake Biwa, Japan. In the surface layer of the sediment from a profundal site, a phylotype closely related to "M. oxyfera" was most frequently detected among NC10 bacteria in PCR analysis of the 16S rRNA gene. In the sediment, sequences related to "M. oxyfera" were also detected in a pmoA gene library. The presence of NC10 bacteria was also confirmed by catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR indicated that the abundance of the "M. oxyfera"-related phylotype was higher in the upper layers of the profundal sediment. The horizontal distribution of the putative methanotrophs in lake sediment was also analyzed by DGGE, which revealed that their occurrence was restricted to deep water areas. These results agreed with those in a previous study of another freshwater lake, and suggested that the upper layer of the profundal sediments is the main habitat for denitrifying methanotrophs. 相似文献
1000.
Tomohiko Kawamura Akinori Yamamoto Kuninao Tada Paul J. Harrison 《Journal of phycology》2012,48(5):1284-1288
Small single‐celled Chaetoceros sp. are often widely distributed, but frequently overlooked. An estuarine diatom with an extremely high growth potential under optimal conditions was isolated from the Shinkawa‐Kasugagawa estuary in the eastern part of the Seto Inland Sea, western Japan. It was identified as Chaetoceros salsugineum based on morphological observations. This strain had a specific growth rate of 0.54 h?1 at 30°C under 700 μmol · m?2 · s?1 (about 30% of natural maximal summer light) with a 14:10 L:D cycle; there was little growth in the dark. However, under continuous light it grew at only 0.35 h?1 or a daily specific growth rate of 8.4 d?1. In addition, cell density, chlorophyll a, and particulate organic carbon concentrations increased by about 1000 times in 24 h at 30°C under 700 μmol · m?2 · s?1 with a 14:10 L:D cycle, showing a growth rate of close to 7 d?1. This very rapid growth rate may be the result of adaptation to this estuarine environment with high light and temperature. Thus, C. salsugineum can be an important primary producer in this estuary in summer and also an important organism for further physiological and genetic research. 相似文献