全文获取类型
收费全文 | 2355篇 |
免费 | 146篇 |
专业分类
2501篇 |
出版年
2023年 | 21篇 |
2022年 | 52篇 |
2021年 | 81篇 |
2020年 | 48篇 |
2019年 | 62篇 |
2018年 | 91篇 |
2017年 | 72篇 |
2016年 | 97篇 |
2015年 | 120篇 |
2014年 | 143篇 |
2013年 | 142篇 |
2012年 | 170篇 |
2011年 | 154篇 |
2010年 | 100篇 |
2009年 | 79篇 |
2008年 | 116篇 |
2007年 | 104篇 |
2006年 | 94篇 |
2005年 | 105篇 |
2004年 | 73篇 |
2003年 | 83篇 |
2002年 | 55篇 |
2001年 | 54篇 |
2000年 | 44篇 |
1999年 | 38篇 |
1998年 | 16篇 |
1997年 | 13篇 |
1996年 | 12篇 |
1995年 | 7篇 |
1994年 | 10篇 |
1993年 | 15篇 |
1992年 | 20篇 |
1991年 | 19篇 |
1990年 | 24篇 |
1989年 | 21篇 |
1988年 | 10篇 |
1987年 | 12篇 |
1986年 | 8篇 |
1985年 | 13篇 |
1984年 | 14篇 |
1983年 | 7篇 |
1981年 | 4篇 |
1978年 | 4篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1974年 | 7篇 |
1973年 | 11篇 |
1972年 | 6篇 |
1971年 | 4篇 |
1969年 | 4篇 |
排序方式: 共有2501条查询结果,搜索用时 0 毫秒
101.
102.
Monte Carlo (MC) molecular simulation describes fluid systems with rich information, and it is capable of predicting many fluid properties of engineering interest. In general, it is more accurate and representative than equations of state. On the other hand, it requires much more computational effort and simulation time. For that purpose, several techniques have been developed in order to speed up MC molecular simulations while preserving their precision. In particular, early rejection schemes are capable of reducing computational cost by reaching the rejection decision for the undesired MC trials at an earlier stage in comparison to the conventional scheme. In a recent work, we have introduced a ‘conservative’ early rejection scheme as a method to accelerate MC simulations while producing exactly the same results as the conventional algorithm. In this paper, we introduce a ‘non-conservative’ early rejection scheme, which is much faster than the conservative scheme, yet it preserves the precision of the method. The proposed scheme is tested for systems of structureless Lennard-Jones particles in both canonical and NVT-Gibbs ensembles. Numerical experiments were conducted at several thermodynamic conditions for different number of particles. Results show that at certain thermodynamic conditions, the non-conservative method is capable of doubling the speed of the MC molecular simulations in both canonical and NVT-Gibbs ensembles. 相似文献
103.
Fecal glucocorticoid metabolites and assay validation: Stress response evaluation in captive brown howler monkeys (Alouatta clamitans) 下载免费PDF全文
104.
In this report, we demonstrate that exonucleolytic turnover is much more important in the regulation of sRNA levels than was previously recognized. For the first time, PNPase is introduced as a major regulatory feature controlling the levels of the small noncoding RNAs MicA and RybB, which are required for the accurate expression of outer membrane proteins (OMPs). In the absence of PNPase, the pattern of OMPs is changed. In stationary phase, MicA RNA levels are increased in the PNPase mutant, leading to a decrease in the levels of its target ompA mRNA and the respective protein. This growth phase regulation represents a novel pathway of control. We have evaluated other ribonucleases in the control of MicA RNA, and we showed that degradation by PNPase surpasses the effect of endonucleolytic cleavages by RNase E. RybB was also destabilized by PNPase. This work highlights a new role for PNPase in the degradation of small noncoding RNAs and opens the way to evaluate striking similarities between bacteria and eukaryotes. 相似文献
105.
Reduced neurotrophic support is one possible cause for retinal ganglion cells dying in glaucoma. Experiments were designed
to investigate the effect of EP2 receptor agonist butaprost on transformed retinal ganglion (RGC-5) cells where reduced neurotrophic
support was simulated by serum withdrawal. Cultures were analysed for cell viability, flow cytometry, reactive oxygen species
and apoptosis. Western blot and immunohistochemistry were used to provide information for the occurrence of PGE2 receptor-types. We demonstrated the existence of all four types of PGE2 receptors in RGC-5 cells and exposure of cultures to butaprost resulted in an elevation of cAMP. Serum deprivation induced
RGC-5 cell death was significantly attenuated by butaprost as well as by rolipram and forskolin where intracellular cAMP levels
were increased. These data are of value in relation to the possible use of EP2 receptor agonists to reduce both elevated intraocular
pressure and retinal ganglion cell death as occurs in glaucoma. 相似文献
106.
Hediat M.H. Salama Ahlam A. Al Watban Anoud T. Al-Fughom 《Saudi Journal of Biological Sciences》2011,18(1):79-86
Investigation was carried out to find whether enhanced ultraviolet radiation influences the Malva parviflora L., Plantago major L., Rumex vesicarius L. and Sismbrium erysimoids Desf. of some annual desert plants. The seeds were grown in plastic pots equally filled with a pre-sieved normal sandy soil for 1 month. The planted pots from each species were randomly divided into equal groups (three groups). Plants of the first group exposed to white-light tubes (400–700 nm) 60 w and UV (365 nm) 8 w tubes. The second group was exposed to white-light tubes (400–700 nm) 60 w and UV (302 nm) 8 w tubes. The third group was exposed to white-light tubes (400–700 nm) 60 w and UV (254 nm) 8 w tubes, respectively, for six days. The results indicated that the chlorophyll contents were affected by enhanced UV radiation. The chlorophyll a, b, and total contents were decreased compared with the control values and reduced with the enhanced UV radiation, but the carotenoid was increased compared with the control and also reduced with the enhanced UV radiation. So, the contents of chlorophylls varied considerably. M. parviflora showed the highest constitutive levels of accumulated chlorophyll a, b, and total chlorophyll (0.463, 0.307 and 0.774 mg g−1 f w) among the investigated plant species. P. major showed the lowest constitutive levels of the chloroplast pigments, 0.0036, 0.0038 and 0.0075 mg g−1 f w for chlorophyll a, b, and total chlorophyll at UV-365 nm, respectively. The protein content was decreased significantly in both root and shoot systems compared with the control values but, it was increased with increasing wave lengths of UV-radiation of all tested plants. R. vesicarius showed the highest protein contents among the investigated plants; its content was 3.8 mg g−1 f w at UV-365 nm in shoot system. On the other hand, decreasing ultraviolet wave length induced a highly significant increase in the level of proline in both root and shoot of all tested plants. From the results obtained, it is suggested that proline can protect cells against damage induced by ultraviolet radiation. Statistically, the variations of the studied metabolic activities were significant due to UV radiation treatment in shoot and root system of all investigated plant species. 相似文献
107.
Comparison of ARM and HEAT protein repeats 总被引:18,自引:0,他引:18
ARM and HEAT motifs are tandemly repeated sequences of approximately 50 amino acid residues that occur in a wide variety of eukaryotic proteins. An exhaustive search of sequence databases detected new family members and revealed that at least 1 in 500 eukaryotic protein sequences contain such repeats. It also rendered the similarity between ARM and HEAT repeats, believed to be evolutionarily related, readily apparent. All the proteins identified in the database searches could be clustered by sequence similarity into four groups: canonical ARM-repeat proteins and three groups of the more divergent HEAT-repeat proteins. This allowed us to build improved sequence profiles for the automatic detection of repeat motifs. Inspection of these profiles indicated that the individual repeat motifs of all four classes share a common set of seven highly conserved hydrophobic residues, which in proteins of known three-dimensional structure are buried within or between repeats. However, the motifs differ at several specific residue positions, suggesting important structural or functional differences among the classes. Our results illustrate that ARM and HEAT-repeat proteins, while having a common phylogenetic origin, have since diverged significantly. We discuss evolutionary scenarios that could account for the great diversity of repeats observed. 相似文献
108.
109.
Lara Saraiva Alanna Silva Reis Jeronimo Marteleto Nunes Rugani Agnes Ant?nia Sampaio Pereira Felipe Dutra Rêgo Ana Cristina Vianna Mariano da Rocha Lima Célia Maria Ferreira Gontijo José Dilermando Andrade Filho 《PloS one》2015,10(8)
Brazil is one of the most important endemic areas for leishmaniasis worldwide. Protected areas that are tourist attractions likely present an important risk of transmission of cutaneous leishmaniasis (CL). Furthermore, with the geographical expansion of visceral leishmaniasis (VL), several studies have recorded the occurrence of its vector, Lutzomyia longipalpis, and cases of human and canine VL in such tourist areas. The Parque Estadual do Sumidouro is an environmentally protected area located in the Brazilian Cerrado biome and in an important area endemic for leishmaniasis in the state of Minas Gerais. The purpose of this study was to monitor the sand fly fauna in areas of tourist activity in the park. Sampling was performed every month, from September 2011 to August 2013, using CDC light traps at six sites of differing environmental characteristics. Sampled specimens were identified following Galati (2003), and females were submitted to molecular techniques for the detection and identification of Leishmania DNA. A total of 4,675 sand fly specimens of 25 species belonging to nine genera were collected. The most abundant species were Micropygomyia quinquefer, Lutzomyia renei and Pintomyia pessoai, although only Pi. pessoai is implicated in the transmission of Leishmania braziliensis. The species accumulation curve reached saturation on the 16th sampling event. Species richness, diversity and evenness differed among the sampled areas. The seasonal curve was not determined by a single unique species, and no single species was the most abundant in all environments sampled. The main vector of Leishmania (Leishmania) infantum, Lutzomyia longipalpis, accounted for only 5.35% of the specimens collected. Proven or suspected vectors of Leishmania (Viannia) braziliensis were recorded, and one female of the cortellezzii complex tested positive for Le. braziliensis DNA. Even with a low infection rate (0.62%), these data indicate the circulation of the parasite and reinforce the need for entomological and epidemiological surveillance in the park and its surroundings. 相似文献
110.
Sugarcane (Saccharum X officinarum): A Reference Study for the Regulation of Genetically Modified Cultivars in Brazil 总被引:1,自引:0,他引:1
Cheavegatti-Gianotto A de Abreu HM Arruda P Bespalhok Filho JC Burnquist WL Creste S di Ciero L Ferro JA de Oliveira Figueira AV de Sousa Filgueiras T Grossi-de-Sá MD Guzzo EC Hoffmann HP de Andrade Landell MG Macedo N Matsuoka S de Castro Reinach F Romano E da Silva WJ de Castro Silva Filho M César Ulian E 《Tropical plant biology》2011,4(1):62-89
Global interest in sugarcane has increased significantly in recent years due to its economic impact on sustainable energy production. Sugarcane breeding and better agronomic practices have contributed to a huge increase in sugarcane yield in the last 30?years. Additional increases in sugarcane yield are expected to result from the use of biotechnology tools in the near future. Genetically modified (GM) sugarcane that incorporates genes to increase resistance to biotic and abiotic stresses could play a major role in achieving this goal. However, to bring GM sugarcane to the market, it is necessary to follow a regulatory process that will evaluate the environmental and health impacts of this crop. The regulatory review process is usually accomplished through a comparison of the biology and composition of the GM cultivar and a non-GM counterpart. This review intends to provide information on non-GM sugarcane biology, genetics, breeding, agronomic management, processing, products and byproducts, as well as the current technologies used to develop GM sugarcane, with the aim of assisting regulators in the decision-making process regarding the commercial release of GM sugarcane cultivars. 相似文献