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991.
Dung Thi Tran Seungchan Cho Phuong Mai Hoang Jaedeok Kim Eui-Joon Kil Taek-Kyun Lee Yong Rhee Sukchan Lee 《Plant Molecular Biology Reporter》2016,34(1):221-232
Chrysanthemum stunt viroid (CSVd), the smallest plant pathogen known to infect chrysanthemums, is a single-stranded circular RNA viroid that induces stunting that results in an overall height reduction of 30–50 % in mature plants. A catalytic single-chain variable antibody, 3D8 scFv, which exhibits intrinsic DNase and RNase activities, was expressed in chrysanthemums to generate transgenic plant resistance to CSVd infection. Moreover, a codon-optimized version of the 3D8 scFv gene for chrysanthemums was also transformed into plants; these codon-optimized transgenic chrysanthemums expressed twice as much 3D8 scFv and displayed 60 % more resistance to CSVd infection, compared with transgenic chrysanthemums harboring the original 3D8 scFv gene. CSVd challenge experiments with codon-optimized and original 3D8 scFv-transgenic chrysanthemums showed that CSVd in newly produced leaves of both codon-optimized and original 3D8 scFv-transgenic plants was not detected by RT-PCR. This is the first report describing the development of a CSVd-resistant chrysanthemum harboring a catalytic single-chain antibody, 3D8 scFv, which has intrinsic RNase activity. 相似文献
992.
This is the first study on the ultrastructure of the zoospores of Ichthyodinium chabelardi, a parasitoid of the fish egg and early larval stages. The zoospores were characterized by the cell structure specific for dinoflagellates; particularly, cells contained large trichocysts and the “dinokaryon”-type nucleus. An unusual large electron-transparent zone was the only significant difference from the “classical” cell structure in Dinoflagellata. We did not find cell structures for the penetration to the host cell (microtubular basket, conoid, or secretory organelles such as rhoptries). The data on the fine structure of the zoospores of I. chabelardi agree with the results of molecular phylogeny; this allows us argue that excluding this species from Dinoflagellata and assigning it to Protalveolata was a mistake. 相似文献
993.
Natalie N. Kinloch Daniel R. MacMillan Anh Q. Le Laura A. Cotton David R. Bangsberg Susan Buchbinder Mary Carrington Jonathan Fuchs P. Richard Harrigan Beryl Koblin Margot Kushel Martin Markowitz Kenneth Mayer M. J. Milloy Martin T. Schechter Theresa Wagner Bruce D. Walker Jonathan M. Carlson Art F. Y. Poon Zabrina L. Brumme 《Journal of virology》2016,90(3):1244-1258
994.
Thanh Thi Ha Dao Emmanuel Nji Abatih Thanh Thi Giang Nguyen Ha Thi Lam Tran Sarah Gabri?l Suzanne Smit Phap Ngoc Le Pierre Dorny 《The Korean journal of parasitology》2016,54(3):357-361
Following the first report of Opisthorchis viverrini infection in a domestic duck in Phu My District of Binh Dinh Province, Central Vietnam, many other cases were observed in the province. We determined the infection rate and intensity of O. viverrini infection in ducks in 4 districts of the province. A total of 178 ducks were randomly selected from 34 farms for examination of flukes in the liver and gall bladder. An infection rate of 34.3% (range 20.7-40.4% among districts) was found; the intensity of infection was 13.8 worms per infected duck (range 1-100). These findings show the role of ducks as a host for O. viverrini, duck genotype, which is sympatric with the human O. viverrini genotype in this province. It also stresses the need for investigations on the zoonotic potential and the life cycle of this parasite. 相似文献
995.
Bui Hong Quang Ritesh Kumar Choudhary Vu Tien Chinh Nguyen The Cuong Do Thi Xuyen Do Van Hai Nong Van Duy Tran Van Tien 《Nordic Journal of Botany》2016,34(6):690-693
A new species of Goniothalamus (Blume) Hook.f. & Thomson (Annonaceae), G. banii B. H. Quang, R. K. Choudhary & V.T. Chinh, is described and illustrated from Thanh Hoa Province, Vietnam. This species shows morphological similarities with G. uvarioides King and G. donnaiensis Finet & Gagnep. but is differentiated by having 22–30 (or more) secondary veins in the leaf, a 1–3‐flowered cyme, a ca 2–3 cm long pedicel, lanceolate and tomentose outer petals, stipitate obovoid‐oblong monocarps, and a single‐seeded monocarp. 相似文献
996.
Xianfang Wu Viet Loan Dao Thi Yumin Huang Eva Billerbeck Debjani Saha Hans-Heinrich Hoffmann Yaomei Wang Luis A. Vale Silva Stephanie Sarbanes Tony Sun Linda Andrus Yingpu Yu Corrine Quirk Melody Li Margaret R. MacDonald William M. Schneider Xiuli An Brad R. Rosenberg Charles M. Rice 《Cell》2018,172(3):423-438.e25
997.
Cations‐induced DNA aggregation can modify the local structure of oligonucleotides and has potential applications in medicine and biotechnology. Here, we used atomic force microscopy to investigate λ‐DNA aggregation on Mg2+‐treated glass (Mg2+/glass) and in Mg2+ solution. Atomic force microscopy topography images showed that some DNA fragments were slightly stacked together on 10 mM Mg2+/glass and stacked stronger on ≥50 mM Mg2+/glass. They also showed that DNA aggregated stronger in Mg2+ solution than on Mg2+/glass, ie, DNAs are strongly stacked and twisted at 10 mM Mg2+, rolled together at 50 mM Mg2+, and slightly aggregated to form small particles at 100 mM Mg2+. At a specific condition, ie, heating λ‐DNA to 92°C, cooling down to 75°C, adding Mg2+, and vortexing the resulting solution, DNA strongly aggregated and formed pancake‐like shapes at 10 and 50 mM or a large aggregate at 100 mM Mg2+ solutions. Our results may be helpful for medical applications and gene therapy using cation‐DNA technology. 相似文献
998.
Quynh-Mai Thi Ngo Thao Quyen Cao Phi-Long Tran Jeong Ah Kim Sang-Tae Seo Jin-Cheol Kim Mi Hee Woo Jeong Hyung Lee Byung Sun Min 《Bioorganic & medicinal chemistry letters》2018,28(11):2109-2115
Five new lactones, litsenolide F1 (1), lisealactone H1 (10), lisealactone H2 (11), akolactone D (13), and akolactone E (14), along with thirteen known compounds were isolated from the pericarps of Litsea japonica (Thunb.) Jussieu. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, HRMS, and chemical methods. The isolated compounds were evaluated for their inhibitory effects on NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Among them, 2-alkylidene-3-hydroxy-4-methylbutanolide derivatives (compounds 1–9) exhibited the most potent activity, with IC50 values in the range of 2.9–12.8?μM. In additon, compounds 1, 3, 4, and 6 showed inhibition of iNOS and COX-2 expression in concentration-dependent manner. Compound 3 suppresses mRNA expression of iNOS, COX-2, IL-6, and TNF-α in LPS-stimulated RAW264.7 cells. Based on these evidence, the isolated lactones from L. japonica could be promissing candidates for the development of new anti-inflammatory agents. 相似文献
999.
Physiological role of urothelial cancer‐associated one long noncoding RNA in human skeletogenic cell differentiation
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1000.
Nguyen Anh Tuan Bui Hong Quang Nguyen Quang Hung Akihiko Sasamoto 《Nordic Journal of Botany》2018,36(3)
A new species, Asarum yentuense N. Tuan & Sasamoto (Aristolochiaceae), is described from Quang Ninh Province, Vietnam. This species belongs to A. subgen. Heterotropa sect. Longistylis and is most similar to Asarum petelotii, A. glabrum, A. reticulatum and A. magnificum var. dinghuense, but is differentiated by morphology, including the length of the calyx tube and lobes, and phenology. 相似文献