Transmissibility of Bacterial Endosymbionts Between Isolates of Acanthamoeba Spp.

ABSTRACT. Experimental transmission of two bacterial endosymbionts to symbiont-free isolates of Acanthamoeba spp. was studied to determine specificity of the host-symbiont relationship. Both symbionts originated from amoebic isolates displaying an identical mitochondrial DNA Eco RI fingerprint (group AcUW II). Symbioses were readily established in one amoebic isolate which displayed a homologous mtDNA fingerprint (group AcUW II). Exposure of a heterologous amoebic isolate (group AcUW IV) to the two symbionts resulted in either cell death or encystation without the establishment of symbioses. While symbioses were established with an amoebic isolate from a second heterologous group (AcUW I), a unique membranous sheath appeared and persisted around one of the symbionts which did not exist in the original host. An isolate representing a third heterologous amoebic group (AcUW VI) was variable in its susceptibility with one symbiont unable to infect the host and the other becoming established only after an initial reaction in which trophozoites rounded-up and floated off the substrate. These studies suggest that a specific recognition system exists between particular isolates of Acanthamoeba and their symbionts, and that the appearance of a killer phenotype is related to contact between mismatched, though recognized, pairs.     

Pollen Production in the Androdioecious Datisca glomerata (Datiscaceae): Implications for Breeding System Equilibrium

Abstract Androdioecy (the presence of males and hermaphrodites in a breeding population) is a rare reproductive system in plants, with Datisca glomerata (Datiscaceae) representing the only well-documented example. Recent reports of high outcrossing rates, inbreeding depression, and high male pollen production satisfy theoretical predictions for the continued maintenance of androdioecy in populations of this species. However, in prior studies pollen production was measured indirectly in terms of numbers of anthers per flower—based on the assumption that male and hermaphroditic plant have equal numbers of flowers and that anthers from the two sexual morphs produce equivalent amounts of pollen. Herein, we demonstrate that male and hermaphrodite plants do not differ significantly in terms of flower number, but that pollen production in anthers from hermaphroditic plants is 12.6% higher than in anthers from male plants, thus refining the estimate of relative pollen fecundity of male versus hermaphrodite plants. The differential lowers the frequency of males predicted by theory, but is still consistent with the maintenance of androdioecy in this species.     

The Protozoan Phylum Opalozoa

ABSTRACT. The recently established protozoan phylum Opalozoa Cavalier-Smith 1991 includes all those zooflagellates with tubular mitochondrial cristae that never have cortical alveoli or rigid tubular ciliary hairs (retronemes), and also the opalinids, proteomyxids sensu stricto, and plasmodiophorids. Opalozoa totally lack plastids but usually (though not invariably) have peroxisomes. They always have well-developed Golgi dictyosomes. The trophic phase is a unicellular ciliated phagotroph except in the only intracellular parasites, the plasmodiophorids, where it is a non-phagotrophic and non-ciliated microplasmodium, and in the proteomyxids where it is an amoeboflagellate (which may sometimes be nonciliated) or a multicellular meroplasmodium. Unlike the phagotrophic Mycetozoa, opalozoans do not form aerial fruiting bodies, but encystation is common. The first detailed classification of the phylum is presented here. It is divided into four subphyla (three new), eight classes (four new, one emended), three subclasses (all new), three superorders (all new) and 22 orders of which 12 are new and one is emended. Diagnoses of these taxa are given, as well as lists of the 31 families (11 new) and 62 genera included within them. Opalozoa, which include Cercomonas and Heterornita , the commonest soil flagellates, are ecologically and evolutionarily important.     

Interspecific competition: A mechanism for rodent succession after fire in wet heathland

Abstract A competitive interaction between two species of sympatric native rodents was demonstrated experimentally in Myall Lakes National Park (NSW, Australia). The previously documented replacement of Pseudomys gracilicaudatus by Rattus lutreolus in areas of wet heath in this region implied that competition may be the mechanism facilitating this succession. We present experimental evidence to support this suggestion. Removal of the larger R. lutreolus caused a significant increase in abundance of P. gracilicaudatus on five experimental sites in comparison to five unmanipulated control sites. The sites used in this experiment were chosen from three ages of wet heath regenerating after fire. These three ages represent key stages in the replacement of P. gracilicaudatus by R. lutreolus and the occurrence of competition in each of these ages indicates that competition plays an important role in this succession. In the initial stages of the experiment when R. lutreolus were removed they were replaced by new R. lutreolus individuals. This has been interpreted as evidence of strong intraspecific competition. After removal of R. lutreolus, P. gracilicaudatus expanded its habitat range into microhabitats that had formerly been occupied by R. lutreolus and reduced its range in microhabitats previously occupied by P. gracilicaudatus. This leads us to believe that P. gracilicaudatus was occupying inferior microhabitat before the removal of R. lutreolus, which had excluded it from more preferable microhabitat.     

Microbiological Synthesis of Fat: THE EFFECTS OF NITROGEN LEVEL ON THE METABOLISM OF PENICILLIUM LILACINUM THOM IN A SUCROSE MEDIUM

1. A study has been made of the relationships between the synthesesof carbohydrate, protein, and fat by Penicillium lilacinum Thomin presence of different amounts of sodium nitrate us a definedsucrose salts medium.
2. Under the defined experimental conditionsincreases in the concentrationof NO^–₂ in the medium werefollowed by increases in therates at which nitrogen and sugarwere taken up by the fungus,in the quantities assimilated,and in total and protein nitrogenin the felt. These conditionsprevailed so long as unassimilatedsugar was available.
3. Mediaof lower NO^–₃ concentration (for example, 0·32or 0·64 per cent. (w/v) NaNO^–₂;) yielded feltsricher in carbohydrate than were those grown in media of higherNO^–₂; content (0·96 or 1·28 per cent. (w/v)NaNO₃ The carbohydrate content of the felts increased graduallyuntil the sugar in the medium was exhausted; carbohydrate contentthen decreased.
4. Media of lower NO^–₃; concentration weremore conduciveto fat synthesis than those of higher NO^–₃;content.

     

Alcohol addiction; problems in treatment

Of about five million problem drinkers in the United States, at least 750,000 are chronic alcoholics, a sixth of whom are in California. Seven phases of drinking patterns are described, from nondependent or "social" drinking, to the last two phases, when blackouts become more frequent and severe, "benders" fade into each other and serious organic diseases appear. General principles of treatment, after medical diagnosis and psychosocial evaluation, include hospitalization for acute episodes and physical disabilities, use of Antabuse (disulfiram) in suitable cases, psychotherapy, and the use of social aids such as Alcoholics Anonymous. The problem requires all resources available in the community, since chronic alcoholics are never cured and need support for long periods. A cooperative patient can finally readjust himself and get along without alcohol. Physicians must recognize the seriousness of alcohol addiction and help patients to accept treatment.