全文获取类型
收费全文 | 1655篇 |
免费 | 45篇 |
国内免费 | 2篇 |
专业分类
1702篇 |
出版年
2015年 | 16篇 |
2013年 | 40篇 |
2012年 | 26篇 |
2011年 | 59篇 |
2010年 | 88篇 |
2009年 | 80篇 |
2008年 | 56篇 |
2007年 | 72篇 |
2006年 | 59篇 |
2005年 | 54篇 |
2004年 | 24篇 |
2003年 | 27篇 |
2001年 | 22篇 |
1999年 | 22篇 |
1998年 | 31篇 |
1997年 | 50篇 |
1996年 | 25篇 |
1995年 | 33篇 |
1994年 | 24篇 |
1993年 | 38篇 |
1992年 | 27篇 |
1991年 | 35篇 |
1990年 | 32篇 |
1989年 | 22篇 |
1988年 | 24篇 |
1987年 | 33篇 |
1986年 | 24篇 |
1985年 | 21篇 |
1984年 | 16篇 |
1983年 | 31篇 |
1982年 | 22篇 |
1981年 | 22篇 |
1980年 | 24篇 |
1979年 | 19篇 |
1978年 | 23篇 |
1977年 | 14篇 |
1975年 | 27篇 |
1974年 | 23篇 |
1973年 | 23篇 |
1972年 | 37篇 |
1971年 | 27篇 |
1958年 | 14篇 |
1957年 | 28篇 |
1956年 | 14篇 |
1955年 | 19篇 |
1954年 | 17篇 |
1953年 | 23篇 |
1952年 | 15篇 |
1951年 | 15篇 |
1950年 | 17篇 |
排序方式: 共有1702条查询结果,搜索用时 15 毫秒
81.
XUBO FEI SHIYONG LU THOMAS BREITHAUPT JÖRG D. HARDEGE JEFFREY L. RAM 《Invertebrate reproduction & development.》2013,57(1-2):69-80
Summary Pheromones can be used as attractants for the opposite sex in many environments; however, little is known about the search strategies employed in responding to pheromones in the marine environment. The spawning behavior of males of the polychaete Nereis succinea is known to be triggered at close range by a high concentration (>~10?7 M) of pheromone, cysteine glutathione disulfide (CSSG), released by females. Since CSSG also causes acceleration of swimming and increased turning, in addition to eliciting ejaculation, we proposed the hypothesis that these behaviors elicited by low concentrations of pheromone can be used by males to find females. The current study develops a computer simulation model of male and female N. succinea behavior for testing whether male responses to low concentrations of CSSG can facilitate finding females. Video recording of female swimming behavior in the field showed spontaneous loops, spirals, and circles that have been incorporated into the model. The scientific workflow paradigm within which the computer model has been developed also incorporates a data provenance system to enable systematic replay and testing of responses to individual parameters. Output of the model shows complex turning behavior leading to successful mating encounters at concentrations as low as 3×10?9 M CSSG. Behavior resembling the output of the model was recorded in field observations. Application of the model in the future will be used to determine what pheromone concentrations produce significant increases in the probability of mating encounters. 相似文献
82.
THOMAS J. KELLY MORTON S. FUCHS SUK-HEE KANG 《Invertebrate reproduction & development.》2013,57(2):101-112
Aedes atropalpus is an autogenous mosquito characterized by a first gonadotropic cycle which results in approximately 200 mature oocytes without a bloodmeal. Ovarian development is completely inhibited if these animals are decapitated or ligated between the thorax and abdomen shortly after adult emergence. Injection of 4.8 ng of 20-hydro- xyecdysone into decapitated females 12 h after eclosion restores ovarian development in all females so treated. However, the same amount of 20-hydroxyecdysone injected into isolated abdomens obtained shortly after adult emergence had no discernible effect on vitellogenesis. In contrast, all abdomens which received 0.5 ng of topically applied JH I followed by the injection of 4.8 ng 20-hydroxyecdysone produced mature oocytes. Isolated abdomens were also capable of oocyte maturation when treated with excess amounts of JH alone; JH I was the most effective followed by JH II and then JH III. Polyacrylamide gel electrophoretic analysis of vitellin extracted from the ovaries of hormonally-treated animals did not reveal any qualitative differences compared to intact normal controls. However, less yolk protein was present in the former. This was verified by counting the number and measuring the size of ovarian follicles in individual females. 相似文献
83.
VIVIANE LANQUAR ASTRID AGORIO JÉRÔME GIRAUDAT THOMAS ROACH ANJA KRIEGER‐LISZKAY SÉBASTIEN THOMINE 《Plant, cell & environment》2013,36(4):804-817
Cadmium (Cd) is highly toxic to plants causing growth reduction and chlorosis. It binds thiols and competes with essential transition metals. It affects major biochemical processes such as photosynthesis and the redox balance, but the connection between cadmium effects at the biochemical level and its deleterious effect on growth has seldom been established. In this study, two Cd hypersensitive mutants, cad1‐3 impaired in phytochelatin synthase (PCS1), and nramp3nramp4 impaired in release of vacuolar metal stores, have been compared. The analysis combines genetics with measurements of photosynthetic and antioxidant functions. Loss of AtNRAMP3 and AtNRAMP4 function or of PCS1 function leads to comparable Cd sensitivity. Root Cd hypersensitivities conferred by cad1‐3 and nramp3nramp4 are cumulative. The two mutants contrast in their tolerance to oxidative stress. In nramp3nramp4, the photosynthetic apparatus is severely affected by Cd, whereas it is much less affected in cad1‐3. In agreement with chloroplast being a prime target for Cd toxicity in nramp3nramp4, the Cd hypersensitivity of this mutant is alleviated in the dark. The Cd hypersensitivity of nramp3nramp4 mutant highlights the critical role of vacuolar metal stores to supply essential metals to plastids and maintain photosynthetic function under Cd and oxidative stresses. 相似文献
84.
Clinal variation in body size and sexual dimorphism in an Indian fruit bat, Cynopterus sphinx (Chiroptera: Pteropodidae) 总被引:1,自引:0,他引:1
JAY F. STORZ J. BALASINGH HARI R. BHAT † P. THIRUCHENTHIL NATHAN D. PARAMANANTHA SWAMI DOSS A. ANTONY PRAKASH THOMAS H. KUNZ 《Biological journal of the Linnean Society. Linnean Society of London》2001,72(1):17-31
Geographic variation in body size and sexual dimorphism of the short‐nosed fruit bat (Cynopterus sphinx) was investigated in peninsular India. Bats were sampled at 12 localities along a 1200 km latitudinal transect that paralleled the eastern flanks of the Western Ghats. The geographic pattern of variation in external morphology of C. sphinx conforms to the predictions of Bergmann's Rule, as indicated by a steep, monotonic cline of increasing body size from south to north. This study represents one of the first conclusively documented examples of Bergmann's Rule in a tropical mammal and confirms that latitudinal clines in body size are not exclusively restricted to temperate zone homeotherms. Body size was indexed by a multivariate axis derived from principal components analysis of linear measurements that summarize body and wing dimensions. Additionally, length of forearm was used as a univariate index of structural size to examine geographic variation in a more inclusive sample of bats across the latitudinal transect. Multivariate and univariate size metrics were strongly and positively correlated with body mass, and exhibited highly concordant patterns of clinal variation. Stepwise multiple regression on climatological variables revealed that increasing size of male and female C. sphinx was associated with decreasing minimum temperature, increasing relative humidity, and increasing seasonality. Although patterns of geographic size variation were highly concordant between the sexes, C. sphinx also exhibited a latitudinal cline in the magnitude and direction of sexual size dimorphism. The size differential reversed direction across the latitudinal gradient, as males averaged larger in the north, and females averaged larger in the south. The degree of female‐biased size dimorphism across the transect was negatively correlated with body size of both sexes. Canonical discriminant analysis revealed that male‐ and female‐biased size dimorphism were based on contrasting sets of external characters. Available data on geographic variation in the degree of polygyny in C. sphinx suggests that sexual selection on male size may play a role in determining the geographic pattern of sexual size dimorphism. 相似文献
85.
以澳洲杨的胚珠及其种子为材料,运用光学显微镜和电镜扫描的方法对其从胚珠发生直到种子成熟的个体发育过程和结构进行观察,同时与鸭脚西番莲的种子附属结构的发育过程进行对比研究。结果表明:(1)澳洲杨的珠孔类型为外珠孔类型,种子附属结构起源于珠孔而非珠柄,其为种阜,而非假种皮。(2)鸭脚西番莲的珠孔类型为内珠孔类型,种子附属结构起源于珠柄,并且最终将珠孔包被,其为真正的假种皮结构。通过种阜与假种皮的不同个体发育过程,建立了大戟科种阜与假种皮的不同发育模式,并对种子附属结构的生物学功能及其暗示的不同植物进化路径进行了讨论。 相似文献
86.
Optical replicas of leaf surfaces were made for characterizingthe lens properties of individual epidermal cells. Using a dentallatex, moulds were made of leaf surfaces and subsequently usedto produce agarose replicas. The replicas focused light in amanner similar to intact epidermal cells and it was possibleto measure both focal lengths and intensifications within leafreplicas of Thermopsis montana, Mahonia repens, and Smilacinastellata which had epidermal cells of different diameter. Focallengths ranged from 74130 µm which indicated thatlight was concentrated within the underlying photosynthetictissues of these leaves. Focal intensifications were measuredsensiometrically and were 1.5 for T. montana and 2-6 for theother species. These values compare favourably with calculatedfocal lengths and measurements taken from isolated epidermallayers. The results indicate that the epidermis can concentratelight within the leaf to amounts well in excess of ambient light.Furthermore, the replicas faithfully reproduced fine anatomicaldetails from a wide variety of leaves and they provide a non-destructiveway to reproduce surface characteristics for anatomical andphysiological studies. 相似文献
87.
88.
89.
Effects of nitrogen on the response of loblolly pine to drought 总被引:2,自引:1,他引:2
90.
THOMAS JÄKEL MARION HENKE BERND WEINGARTEN DAMARIS KLIEMT SILVIA SEIDINGER 《The Journal of eukaryotic microbiology》1997,44(4):293-299
To establish an in vitro culture system for the precystic phase of Sarcocystis singaporensis, we initially tested various excysting fluids for sporocysts. An excysting fluid containing 2.5% bovine taurocholate and 10% bile of the specific intermediate host, Rattus norvegicus, in RPMI medium was the most suitable resulting in excystation of 80% of the sporozoites. Subsequently, we identified brain endothelial cells and pneumonocytes of the rat to promote growth of sporozoites to schizonts. Hepatoma, fibroblastic, or myoblastic cells were not suitable for the parasite's development. First-generation schizonts were seen at days 3-10 postinoculation (PI); a distinct second peak of schizogonic development only occurred in endothelial cells at days 14-18 PI. First-generation schizonts were 26.0 (± 3.8) μm in diameter and contained 32-50 merozoites, second-generation schizonts measured 34.4 (± 10.6) μm and contained 54-72 merozoites. Merozoite yield at large-scale culture conditions (75 cm2 flasks) using pneumonocytes as host cells was relatively low. Ultrastructurally, sporozoites and merozoites were quite similar to corresponding stages of other Sarcocystis species. With regard to host cell specificity and developmental kinetics, in vitro cultivation showed close similarities to the situation in vivo. 相似文献