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BACKGROUND: Biochemical testing for pheochromocytoma by measurement of fractionated plasma metanephrines is limited by false positive rates of up to 18% in people without known genetic predisposition to the disease. The plasma normetanephrine fraction is responsible for most false positives and plasma normetanephrine increases with age. The objective of this study was to determine if we could improve the specificity of fractionated plasma measurements, by statistically adjusting for age. METHODS: An age-adjusted metanephrine score was derived using logistic regression from 343 subjects (including 33 people with pheochromocytoma) who underwent fractionated plasma metanephrine measurements as part of investigations for suspected pheochromocytoma at Mayo Clinic Rochester (derivation set). The performance of the age-adjusted score was validated in a dataset of 158 subjects (including patients 23 with pheochromocytoma) that underwent measurements of fractionated plasma metanephrines at Mayo Clinic the following year (validation dataset). None of the participants in the validation dataset had known genetic predisposition to pheochromocytoma. RESULTS: The sensitivity of the age-adjusted metanephrine score was the same as that of traditional interpretation of fractionated plasma metanephrine measurements, yielding a sensitivity of 100% (23/23, 95% confidence interval [CI] 85.7%, 100%). However, the false positive rate with traditional interpretation of fractionated plasma metanephrine measurements was 16.3% (22/135, 95% CI, 11.0%, 23.4%) and that of the age-adjusted score was significantly lower at 3.0% (4/135, 95% CI, 1.2%, 7.4%) (p < 0.001 using McNemar's test). CONCLUSION: An adjustment for age in the interpretation of results of fractionated plasma metanephrines may significantly decrease false positives when using this test to exclude sporadic pheochromocytoma. Such improvements in false positive rate may result in savings of expenditures related to confirmatory imaging. 相似文献
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JIA ZHI‐YING SUN XIAO‐WEN LIANG LI‐QUN LI DA‐YU LEI QING‐QUAN 《Molecular ecology resources》2006,6(4):1282-1284
We report the development of 11 polymorphic microsatellite loci in pacific white shrimp (Litopenaeus vannamei) using an unenriched genomic library. The number of the alleles ranged from two to 18 and observed hererozygosity ranged from 0.0286 to 0.9429, indicating that these markers will be useful for population studies and mapping in pacific white shrimp. Seven loci were detected deviated from Hardy–Weinberg, caused by deficiency of heterozygote, suggesting population genetic structure across the sampled population. No evidence for linkage disequilibrium was found. 相似文献
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ANDERS GONÇALVES
Da SILVA JESSICA R. EBERHARD TIMOTHY F. WRIGHT MICHAEL L. AVERY MICHAEL A. RUSSELLO 《Molecular ecology》2010,19(16):3336-3350
The monk parakeet (Myiopsitta monachus) is a successful invasive species that does not exhibit life history traits typically associated with colonizing species (e.g., high reproductive rate or long‐distance dispersal capacity). To investigate this apparent paradox, we examined individual and population genetic patterns of microsatellite loci at one native and two invasive sites. More specifically, we aimed at evaluating the role of propagule pressure, sexual monogamy and long‐distance dispersal in monk parakeet invasion success. Our results indicate little loss of genetic variation at invasive sites relative to the native site. We also found strong evidence for sexual monogamy from patterns of relatedness within sites, and no definite cases of extra‐pair paternity in either the native site sample or the examined invasive site. Taken together, these patterns directly and indirectly suggest that high propagule pressure has contributed to monk parakeet invasion success. In addition, we found evidence for frequent long‐distance dispersal at an invasive site (~100 km) that sharply contrasted with previous estimates of smaller dispersal distance made in the native range (~2 km), suggesting long‐range dispersal also contributes to the species’ spread within the United States. Overall, these results add to a growing body of literature pointing to the important role of propagule pressure in determining, and thus predicting, invasion success, especially for species whose life history traits are not typically associated with invasiveness. 相似文献
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PAULO R. GUIMARES RAFAEL L. G. RAIMUNDO CLUDIA BOTTCHER ROGRIO R. SILVA JOS R. TRIGO 《Austral ecology》2006,31(6):776-782
Abstract In several plants, extrafloral nectaries (EFN) are located close to the reproductive structures, suggesting that ants may act as a defence against specialized seed predators that overcome chemical defences. Alternatively, ants may also deter herbivores in a generalized manner, thereby protecting the whole plant. In this work, we examined the relationship between the chemically protected weed Crotalaria pallida Ait. (Leguminosae) that bears EFN, its specialized seed predator, the larvae of the arctiid moth Utetheisa ornatrix L. (Arctiidae) and ants. We tested two hypotheses related to the type of deterrence caused by ants. The Seed Predator Deterrence Hypothesis predicts that ant deterrence is directed primarily towards herbivores that destroy seeds and other reproductive structures, without attacking herbivores on vegetative structures. The General Deterrence Hypothesis states that ants are general in their effects, equally deterring herbivores in vegetative and reproductive structures. Our results supported the predictions of the Seed Predator Deterrence Hypothesis, namely, that (i) ant activity on EFN was related to the vulnerability of reproductive structures to attack by U. ornatrix; (ii) ant patrolling was restricted almost entirely to racemes; (iii) ants removed termites used as baits more frequently on racemes than on leaves; and (iv) U. ornatrix larvae were often expulsed from the racemes. These results indicate that EFN can act as another deterrent mechanism in chemically protected plants by promoting the expulsion of specialist seed predators. 相似文献
47.
ANA SANTOS EDUARDO MAYORAL B. GUDVEIG BAARLI CARLOS M.
Da SILVA MÁRIO CACHÃO MARKES E. JOHNSON 《Palaeontology》2012,55(1):173-182
Abstract: The bioclaustration of the pyrgomatid barnacle Ceratoconcha aff. costata within the carbonate skeleton of a colonial hermatypical coral (Tarbellastrea reussiana) is described from a middle Miocene basaltic rocky palaeoshore on a small north‐eastern Atlantic islet of Porto Santo (Madeira Archipelago, Portugal). The resulting structure is named as a new trace fossil Imbutichnus igen. nov., characterized by a small, funnel‐like cavity, a circular to oval cross‐section, with a nearly cylindrical shape in the upper part and a conical shape towards the base. Imbutichnus costatum isp. nov. is defined as a bioclaustration structure produced by the overgrowth of a pyrgomatid barnacle by a coral. From an ethologic point of view, Imbutichnus is attributed to the Impedichnia category. In terms of palaeoecology, it is interpreted as the result of a parasitic relationship. This is also the oldest record of pyrgomatid barnacles in the eastern Atlantic and clearly demonstrates that the Miocene palaeogeographic range of Ceratoconcha was much wider than previously assumed. 相似文献
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DANIEL APOLÔNIO SILVA DE OLIVEIRA WILFRIDA DECRAEMER OLEKSANDER HOLOVACHOV JAY BURR IRMA TANDINGAN DE LEY PAUL DE LEY TOM MOENS SOFIE DERYCKE 《Zoological Journal of the Linnean Society》2012,164(1):18-35
Nematode diversity may seriously be underestimated when taking into account cryptic speciation. Thoracostoma trachygaster is commonly found in kelp holdfasts along the California coastline and was recently shown to consist of at least two distinct molecular clades (I and II). Here, we provide detailed morphological analysis of both clades, based on measurements taken from video vouchers of respectively eight and 16 individuals from the previous study, as well as 80 newly collected specimens from four Californian beaches. The latter were vouchered, measured, and then subjected to molecular analyses of the mitochondrial cytochrome oxidase c subunit I (COI) gene, and the ribosomal D2D3 and internal transcribed spacer (ITS) regions. This integrative approach shows that the three molecular clades are phylogenetically and morphologically distinct species, but a combination of morphological characters is needed to distinguish them. Two new species, Thoracostoma fatimae sp. nov. and Thoracostoma igniferum sp. nov. , are identified and described. The spicule length of T. fatimae sp. nov. is significantly shorter than that of T. trachygaster. Thoracostoma igniferum sp. nov. can be distinguished by the irregular posterior edge of the cephalic capsule and the two internal subdorsal tropis‐like projections in the wall of the cephalic capsule, which are lacking in T. fatimae sp. nov. and T. trachygaster. © 2012 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 164 , 18–35. 相似文献
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S. V. CUADRA M. H. COSTA C. J. KUCHARIK H. R. DA ROCHA J. D. TATSCH G. INMAN‐BAMBER R. P. DA ROCHA C. C. LEITE O. M. R. CABRAL 《Global Change Biology Bioenergy》2012,4(1):36-48
Scientists predict that global agricultural lands will expand over the next few decades due to increasing demands for food production and an exponential increase in crop‐based biofuel production. These changes in land use will greatly impact biogeochemical and biogeophysical cycles across the globe. It is therefore important to develop models that can accurately simulate the interactions between the atmosphere and important crops. In this study, we develop and validate a new process‐based sugarcane model (included as a module within the Agro‐IBIS dynamic agro‐ecosystem model) which can be applied at multiple spatial scales. At site level, the model systematically under/overestimated the daily sensible/latent heat flux (by ?10.5% and 14.8%, H and λE, respectively) when compared against the micrometeorological observations from southeast Brazil. The model underestimated ET (relative bias between ?10.1% and –12.5%) when compared against an agro‐meteorological field experiment from northeast Australia. At the regional level, the model accurately simulated average yield for the four largest mesoregions (clusters of municipalities) in the state of São Paulo, Brazil, over a period of 16 years, with a yield relative bias of ?0.68% to 1.08%. Finally, the simulated annual average sugarcane yield over 31 years for the state of Louisiana (US) had a low relative bias (?2.67%), but exhibited a lower interannual variability than the observed yields. 相似文献