Molecular cloning and structural analysis of human norepinephrine transporter gene（NETHG）

A cDNA molecule encoding a major part of the human Norepinephrine transporter(hNET) was synthesized by means of Polymerase Chain Reaction(PCR) technique and used as a probe for selecting the human genomic NET gene.A positive clone harbouring the whole gene was obtained from a human lymphocyte genomic library through utilizing the “genomic walking” technique.The clone,designated as phNET,harbours a DNA fragment of about 59 kd in length inserted into BamH I site in cosmid pWE15.The genomic clone contains 14 exons encoding all amino acid residues in the protein.A single exon encodes a distinct transmembrane domain,except for transmembrane domain 10 and 11,which are encoded by part of two exons respectively,and exon 12,which encodes part of domain 11 and all of domain 12.These results imply that there is a close relationship between exon splicing of a gene and structureal domains of the protein,as is the case for the human γ－aminobutyric acid transporter(hGAT) and a number of other membrane proteins.     

Cloning and sequencing of mouse GABA transporter complementary DNA

A cDNA encoding the mouse GABA transporter has been isolated and sequenced.The results show that the mouse GABA transporter cDNA differs from that of the rat by 60 base pairs at the open reading frame region but the deduced amino acid sequences of the two cDNAs are identical and both composed of 599 amino acids.However,the amino acid sequence is different from the sequence deduced from a recently published mouse GABA transporter cDNA.     

深圳红树林湿地浮游植物多样性的组成与分布

根据深圳福田红树林湿地的浮游植物周年调查资料,分析浮游植物群落多样性的组成与时空分布特征,并利用浮游植物群落多样性指数对湿地水体的营养状态进行评价。共记录浮游植物5门28属51种,各季度浮游植物数量均超过10.6 ind·L^-1。硅藻分布广泛,在种类和数量上均占主导地位,优势种为微小小环藻（Cyclotella caspia）和诺氏海链藻（Thalassiosira nordenskiöldi）等,耐受污染的藻类,如小颤藻（Oscillatoria minima）、鱼形裸藻（Euglena pisciformis）在夏秋2季成为个别站位的优势种。浮游植物种类组成时空变化明显,而数量的空间变化较大、季节变化不大,优势种的时空变化明显,但种类单一,多样性指数的空间变化较大。群落多样性指数评价结果显示,深圳红树林湿地处于中-富营养化状态,有向富营养化过渡的趋势,应加强对内陆径流和污水排放进入红树林湿地的严格控制和管理。