Effect of Evaporative Demand on Water Relations of Citrus limon

Conductance, transpirational flux and xylem pressure potentialwere measured in leaves of well-watered 5-year-old lemon trees(Citrus limon (L.) Burm. f.) subjected to different levels ofevaporative demand. Increased leaf-to-air absolute humiditydifference generally decreased stomatal conductance and increasedxylem pressure potential, with a good correlation between thelast two parameters; but this trend was reversed on days withvery high evaporative demand, when stomata opened in spite ofthe low humidity. Citrus limon (L.) Burm. f., lemon, water stress, stomatal conductance, leaf water potential, transpiration, air humidity     

Cl‐ homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes

To reveal specific Cl^‐ transport activities in the symplastic pathway, uptake, long‐distance transport and distribution of Cl^‐ have been investigated in the citrus rootstocks Carrizo citrange (CC, Cl^‐ includer) and Cleopatra mandarin (CM, Cl^‐ excluder). Using an external concentration of 4.5 mm Cl^‐, both species actively transported Cl^‐ to levels that exceeded the critical requirement concentration by one and two orders of magnitude in the excluder and the includer rootstocks, respectively. Both CC and CM modulated Cl^‐ influx according to the availability of the nutrient as uptake capacity was induced by Cl^‐ starvation, but inhibited after Cl^‐ resupply. Net Cl^‐ uptake was higher in the includer CC, an observation that correlated with a lower root‐to‐shoot transport capacity in the excluder CM. The patterns of tissue Cl^‐ accumulation indicated that chloride exclusion in the salt‐tolerant rootstock CM was caused by a reduced net Cl^‐ loading into the root xylem. Genes CcCCC1, CcSLAH1 and CcICln1 putatively involved in the regulation of chloride transport were isolated and their expression analysed in response to both changes in the nutritional status of Cl^‐ and salt stress. The previously uncharacterized ICln gene exhibited a strong repression to Cl^‐ application in the excluder rootstock, suggesting a role in regulating Cl^‐ homeostasis in plants.     

The use of S1 nuclease treatment of hybrid PCR products for the differentiation between PVY isolates

A new strategy based on treating PCR hybrids with S1 nuclease was used to differentiate between two PVY isolates. Mixed denatured and annealed hybrid PCR products of two PVY isolates including a tested strain and a reference N strain were treated with S1 nuclease. Single-stranded mismatched regions were revealed by the S1 nuclease cleavage, yielding a characteristic pattern of bands in polyacrylamide gel by which virus isolates could be matched. Sequence analysis of the relevant PCR products revealed that only part of the mismatched regions were cleaved by the S1 nuclease. Still, the distinct pattern of degradation products enabled the differentiation between the PVY isolates. The general application of this procedure for strain differentiation is discussed.     

Cloning of a Partial Length cDNA Encoding the C-Terminal Portion of the 75-77-kDa Antigen of Trypanosoma cruzi

ABSTRACT It has been suggested that several Trypanosoma cruzi antigens have possible protective epitopes which may be suitable vaccine candidates. We found previously that animals resistant to T. cruzi infection produced antibodies against the 75-77-kDa parasite antigen. To test the ability of the recombinant form of this antigen to protect animals from T. cruzi infection, the cDNA which encodes a portion of the 75-77-kDa antigen was cloned using a cDNA library constructed in an orientation-specific bacteriophage expression vector (λgt11) from poly (A)⁺ RNA of Brazil strain epimastigotes. One clone, named SFS-40, was selected by screening the library using affinity purified antibodies specific for the 75-77-kDa parasite antigen as probe. The cDNA corresponding to the 1.7-kilobase insert of SFS-40 was subcloned into plasmid vectors and characterized. The cDNA sequence encodes a polypeptide of about 40 kDa. The putative product of the cDNA was homologous to members of the 70-kDa stress protein family. When epimastigotes were shifted from 29° C to 37° C, there was no change in the level of SFS-40 mRNA. In contrast, the 70-kDa heat shock protein mRNA of the parasite was increased about four fold by this treatment.