全文获取类型
收费全文 | 685402篇 |
免费 | 79064篇 |
国内免费 | 855篇 |
出版年
2018年 | 6054篇 |
2016年 | 8141篇 |
2015年 | 11408篇 |
2014年 | 13454篇 |
2013年 | 18323篇 |
2012年 | 21325篇 |
2011年 | 21947篇 |
2010年 | 14559篇 |
2009年 | 13534篇 |
2008年 | 19523篇 |
2007年 | 20107篇 |
2006年 | 19090篇 |
2005年 | 18300篇 |
2004年 | 18264篇 |
2003年 | 17271篇 |
2002年 | 16914篇 |
2001年 | 32653篇 |
2000年 | 32807篇 |
1999年 | 25826篇 |
1998年 | 8769篇 |
1997年 | 8982篇 |
1996年 | 8461篇 |
1995年 | 7969篇 |
1994年 | 7697篇 |
1993年 | 7753篇 |
1992年 | 21036篇 |
1991年 | 20810篇 |
1990年 | 20134篇 |
1989年 | 19492篇 |
1988年 | 18208篇 |
1987年 | 17138篇 |
1986年 | 16064篇 |
1985年 | 15645篇 |
1984年 | 12769篇 |
1983年 | 11075篇 |
1982年 | 8201篇 |
1981年 | 7302篇 |
1980年 | 6868篇 |
1979年 | 11859篇 |
1978年 | 9442篇 |
1977年 | 8487篇 |
1976年 | 7787篇 |
1975年 | 8952篇 |
1974年 | 9644篇 |
1973年 | 9451篇 |
1972年 | 8377篇 |
1971年 | 7805篇 |
1970年 | 6727篇 |
1969年 | 6592篇 |
1968年 | 5919篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
911.
912.
913.
Complete amino acid sequence of a novel integrin beta subunit (beta 6) identified in epithelial cells using the polymerase chain reaction 总被引:18,自引:0,他引:18
D Sheppard C Rozzo L Starr V Quaranta D J Erle R Pytela 《The Journal of biological chemistry》1990,265(20):11502-11507
The integrin family of adhesion receptors consists of several heterodimeric glycoproteins, each composed of one alpha and one beta subunit. Three different mammalian beta subunits, beta 1, beta 2, and beta 3, have been sequenced, but recent evidence suggests the existence of several others. Amplification of guinea pig airway epithelial cell cDNA with oligonucleotide primers designed to recognize consensus integrin beta subunit sequences led to the identification of a novel partial cDNA sequence. Clones containing portions of this sequence were used to screen cDNA libraries constructed from the human pancreatic carcinoma cell line FG-2 and identified a series of overlapping clones encoding the full-length sequence of the human homologue of this protein. This sequence of 788 amino acids is 43, 38, and 47% identical to the sequences of beta 1, beta 2, and beta 3, respectively. Features shared between this novel protein and the previously sequenced beta subunits include the positions of all 56 cysteine residues in the extracellular domain, the single putative transmembrane domain, and the short putative cytoplasmic domain. However, a unique 11-amino acid extension at the carboxyl terminus, not present in any of the other beta subunits, is suggestive of distinctive interactions with cytoplasmic components. Comparison of the human and guinea pig sequences reveals a high degree (94%) of cross-species conservation. Because this protein is clearly distinct from the two other recently described integrins beta 4 and beta 5, we propose to designate it beta 6. 相似文献
914.
915.
Hermógenes Fernández-Marín David R. Nash Sarah Higginbotham Catalina Estrada Jelle S. van Zweden Patrizia d'Ettorre William T. Wcislo Jacobus J. Boomsma 《Proceedings. Biological sciences / The Royal Society》2015,282(1807)
Fungus-farming ant colonies vary four to five orders of magnitude in size. They employ compounds from actinomycete bacteria and exocrine glands as antimicrobial agents. Atta colonies have millions of ants and are particularly relevant for understanding hygienic strategies as they have abandoned their ancestors'' prime dependence on antibiotic-based biological control in favour of using metapleural gland (MG) chemical secretions. Atta MGs are unique in synthesizing large quantities of phenylacetic acid (PAA), a known but little investigated antimicrobial agent. We show that particularly the smallest workers greatly reduce germination rates of Escovopsis and Metarhizium spores after actively applying PAA to experimental infection targets in garden fragments and transferring the spores to the ants'' infrabuccal cavities. In vitro assays further indicated that Escovopsis strains isolated from evolutionarily derived leaf-cutting ants are less sensitive to PAA than strains from phylogenetically more basal fungus-farming ants, consistent with the dynamics of an evolutionary arms race between virulence and control for Escovopsis, but not Metarhizium. Atta ants form larger colonies with more extreme caste differentiation relative to other attines, in societies characterized by an almost complete absence of reproductive conflicts. We hypothesize that these changes are associated with unique evolutionary innovations in chemical pest management that appear robust against selection pressure for resistance by specialized mycopathogens. 相似文献
916.
917.
David L. Remington 《Genetics》2009,181(3):1087-1099
The use of high-throughput genomic techniques to map gene expression quantitative trait loci has spurred the development of path analysis approaches for predicting functional networks linking genes and natural trait variation. The goal of this study was to test whether potentially confounding factors, including effects of common environment and genes not included in path models, affect predictions of cause–effect relationships among traits generated by QTL path analyses. Structural equation modeling (SEM) was used to test simple QTL-trait networks under different regulatory scenarios involving direct and indirect effects. SEM identified the correct models under simple scenarios, but when common-environment effects were simulated in conjunction with direct QTL effects on traits, they were poorly distinguished from indirect effects, leading to false support for indirect models. Application of SEM to loblolly pine QTL data provided support for biologically plausible a priori hypotheses of QTL mechanisms affecting height and diameter growth. However, some biologically implausible models were also well supported. The results emphasize the need to include any available functional information, including predictions for genetic and environmental correlations, to develop plausible models if biologically useful trait network predictions are to be made. 相似文献
918.
919.
920.
The blue and the black wildebeest, Connochaetes taurinus and C. gnou, are currently classified as congeneric, but previous reports have placed C. taurinus in its own genus, Gorgon. To further clarify the evolutionary relationship between these two species, we examined and compared their mitotic chromosomes and mitochondrial DNA (mtDNA). No species-specific G-banded or C-banded chromosomal markers were found, and we conclude that the karyotypes are invariant at the level of resolution obtained. An evolutionary divergence time of approximately 1 million years was calculated from mtDNA restriction fragment data, indicating a close phylogenetic relationship for the two wildebeest species. The low nucleotide diversity detected within the black wildebeest (0.09%) is thought to reflect the recent population bottleneck to which the species has been subjected. In contrast, the limited heterogeneity (0.02%) within the South African blue wildebeest herds sampled in this study was surprising, and we argue that for many populations, especially those on smaller reserves, this may reflect common descent from a small number of animals through management-controlled translocations. 相似文献