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71.
Sylwia Flis Agnieszka Gnyszka Irena Misiewicz-Krzemińska Jacek Spławiński 《Cancer cell international》2009,9(1):10-10
Background
DNA methylation is an epigenetic phenomenon known to play an important role in the development of cancers, including colorectal cancer (CRC). Aberrant methylation of promoter regions of genes is potentially reversible, and if methylation is important for cancer survival, demethylation should do the opposite. To test this we have addressed the hypothesis that DNA methyltransferase inhibitors (DNMTi), decytabine and zebularine, potentiate inhibitory effects of classical anti-CRC cytostatics, oxaliplatin and 5-fluorouracil (5-FU), on survival of CRC cells in vitro. 相似文献72.
73.
Jezierska Sylwia Van Bogaert Inge N. A. 《Journal of industrial microbiology & biotechnology》2017,44(4-5):721-733
Journal of Industrial Microbiology & Biotechnology - How small molecules cross cellular membranes is an often overlooked issue in an industrial microbiology and biotechnology context. This is... 相似文献
74.
Wolf population genetics in Europe: a systematic review,meta‐analysis and suggestions for conservation and management 下载免费PDF全文
Maris Hindrikson Jaanus Remm Malgorzata Pilot Raquel Godinho Astrid Vik Stronen Laima Baltrūnaité Sylwia D. Czarnomska Jennifer A. Leonard Ettore Randi Carsten Nowak Mikael Åkesson José Vicente López‐Bao Francisco Álvares Luis Llaneza Jorge Echegaray Carles Vilà Janis Ozolins Dainis Rungis Jouni Aspi Ladislav Paule Tomaž Skrbinšek Urmas Saarma 《Biological reviews of the Cambridge Philosophical Society》2017,92(3):1601-1629
The grey wolf (Canis lupus) is an iconic large carnivore that has increasingly been recognized as an apex predator with intrinsic value and a keystone species. However, wolves have also long represented a primary source of human–carnivore conflict, which has led to long‐term persecution of wolves, resulting in a significant decrease in their numbers, genetic diversity and gene flow between populations. For more effective protection and management of wolf populations in Europe, robust scientific evidence is crucial. This review serves as an analytical summary of the main findings from wolf population genetic studies in Europe, covering major studies from the ‘pre‐genomic era’ and the first insights of the ‘genomics era’. We analyse, summarize and discuss findings derived from analyses of three compartments of the mammalian genome with different inheritance modes: maternal (mitochondrial DNA), paternal (Y chromosome) and biparental [autosomal microsatellites and single nucleotide polymorphisms (SNPs)]. To describe large‐scale trends and patterns of genetic variation in European wolf populations, we conducted a meta‐analysis based on the results of previous microsatellite studies and also included new data, covering all 19 European countries for which wolf genetic information is available: Norway, Sweden, Finland, Estonia, Latvia, Lithuania, Poland, Czech Republic, Slovakia, Germany, Belarus, Russia, Italy, Croatia, Bulgaria, Bosnia and Herzegovina, Greece, Spain and Portugal. We compared different indices of genetic diversity in wolf populations and found a significant spatial trend in heterozygosity across Europe from south‐west (lowest genetic diversity) to north‐east (highest). The range of spatial autocorrelation calculated on the basis of three characteristics of genetic diversity was 650?850 km, suggesting that the genetic diversity of a given wolf population can be influenced by populations up to 850 km away. As an important outcome of this synthesis, we discuss the most pressing issues threatening wolf populations in Europe, highlight important gaps in current knowledge, suggest solutions to overcome these limitations, and provide recommendations for science‐based wolf conservation and management at regional and Europe‐wide scales. 相似文献
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76.
Sławomir Borek Szymon Kubala Sylwia Kubala Lech Ratajczak 《Acta Physiologiae Plantarum》2011,33(5):1953-1968
Storage lipid and protein breakdown in germinating seeds of yellow (Lupinus luteus L.), white (L. albus L.), and Andean lupine (L. mutabilis Sweet) and regulatory function of sucrose were investigated. Less oil bodies were detected in organs of yellow lupine seeds,
whereas the highest content of oil bodies was noticed in the Andean lupine seeds. Mature, air-dried yellow, white and Andean
lupine seeds do not contain starch. Starch grains appear the earliest in white lupine seeds during imbibition. Sucrose deficiency
in tissues enhances breakdown of storage lipid, protein and temporary starch in cotyledons. In sucrose starved embryo axes
of all investigated lupine species, an increased level of vacuolization was noted. Interconnections between catabolism of
storage protein and storage lipid in germinating lupine seeds were identified by applying 14C-acetate. To assess the importance of key processes in storage lipid breakdown NaF (inhibitor of glycolysis and gluconeogenesis),
KCN, NaN3 and SHAM (inhibitors of mitochondrial electron transport chain) and MSO (inhibitor of glutamine synthetase) were used. Radioactivity
coming from 14C-acetate was released as 14CO2 but mostly was incorporated into ethanol-soluble fraction of embryo axes and cotyledons. Respiratory inhibitors caused a
significant decrease in 14CO2 and ethanol fractions in all three lupine species studied. MSO stimulated release of 14CO2 and radioactivity of ethanol fractions in yellow lupine organs fed with sucrose, but in Andean lupine MSO enhanced the production
of 14CO2 and radioactivity of ethanol fractions both in organs fed and not fed with sucrose. Different strategies of storage compound
breakdown are proposed, depending on relative proportion in storage protein and lipid content in lupine seeds. 相似文献
77.
Staniszewska M Słuczanowska-Głąbowska S Drukała J 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》2011,49(3):375-380
Stem cells represent a great hope for regenerative medicine. In adult life, stem cell deposits are kept in organ niches; the need for tissue or organ regeneration mobilizes stem cells via the SDF-1-CXCR4 regulation axis. Constant regeneration of the skin is achieved due to stem cell differentiation within the epidermis and the hair follicle; thus, skin may serve as an excellent source of stem cells. This is of paramount importance in the treatment of chronic skin wounds and burns. 相似文献
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Bartoszewski R Brewer JW Rab A Crossman DK Bartoszewska S Kapoor N Fuller C Collawn JF Bebok Z 《The Journal of biological chemistry》2011,286(48):41862-41870
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Sylwia Jancowski Amanda Catching Jamie Pighin Takamasa Kudo Ilse Foissner Geoffrey O. Wasteneys 《The Plant journal : for cell and molecular biology》2014,77(4):497-510
Proteins detrimental to endoplasmic reticulum (ER) morphology need to be efficiently exported. Here, we identify two mechanisms that control trafficking of Arabidopsis thalianaGLL23, a 43 kDa GDSL‐like lipase implicated in glucosinolate metabolism through its association with the β‐glucosidase myrosinase. Using immunofluorescence, we identified two mutants that showed aberrant accumulation of GLL23: large perinuclear ER aggregates in the nuclear cage (nuc) mutant; and small compartments contiguous with the peripheral ER in the cytoplasmic bodies (cyb) mutant. Live imaging of fluorescently tagged GLL23 confirmed its presence in the nuc and cyb compartments, but lack of fluorescent signals in the wild‐type plants suggested that GLL23 is normally post‐translationally modified for ER export. NUC encodes the MVP1/GOLD36/ERMO3 myrosinase‐associated protein, previously shown to have vacuolar distribution. CYB is an ER and Golgi‐localized p24 type I membrane protein component of coat protein complex (COP) vesicles, animal and yeast homologues of which are known to be involved in selective cargo sorting for ER–Golgi export. Without NUC, GLL23 accumulates in the ER this situation suggests that NUC is in fact active in the ER. Without CYB, both GLL23 and NUC were found to accumulate in cyb compartments, consistent with a role for NUC in GLL23 processing and indicated that GLL23 is the likely sorting target of the CYB p24 protein. 相似文献
80.
Microtubules are subcellular nanotubes composed of α- and β-tubulin that arise from microtubule nucleation sites and are mainly composed of γ-tubulin complexes. Cell wall encased plant cells have evolved four distinct microtubule arrays that regulate cell division and expansion. Microtubule-associated proteins, the so called MAPs, construct, destruct and reorganize microtubule arrays thus regulating their spatiotemporal transitions during the cell cycle. By physically binding to microtubules and/or modulating their functions, MAPs control microtubule dynamic instability and/or interfilament cross talk. We survey the recent analyses of Arabidopsis MAPs such as MAP65, MOR1, CLASP, katanin, TON1, FASS, TRM, TAN1 and kinesins in terms of their effects on microtubule array organizations and plant development. 相似文献