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881.
Loubéry S Wilhelm C Hurbain I Neveu S Louvard D Coudrier E 《Traffic (Copenhagen, Denmark)》2008,9(4):492-509
Important progress has been made during the past decade in the identification of molecular motors required in the distribution of early and late endosomes and the proper trafficking along the endocytic pathway. There is little direct evidence, however, that these motors drive movement of the endosomes. To evaluate the contributions of kinesin-1, dynein and kinesin-2 to the movement of early and late endosomes along microtubules, we made use of a cytosol-free motility assay using magnetically isolated early and late endosomes as well as biochemical analyses and live-cell imaging. By making use of specific antibodies, we confirmed that kinesin-1 and dynein move early endosomes and we found that kinesin-2 moves both early and late endosomes in the cell-free assay. Unexpectedly, dynein did not move late endosomes in the cell-free assay. We provide evidence from disruption of dynein function and latrunculin A treatment, suggesting that dynein regulates late endosome movement indirectly, possibly through a mechanism involving the actin cytoskeleton. These data provide new insights into the complex regulation of endosomes' motility and suggest that dynein is not the major motor required to move late endosomes toward the minus end of microtubules. 相似文献
882.
Rising economic and ecological costs caused by invasive organisms revived research on biological attributes associated with invasiveness, focussing on the question: do invasive taxa have biological attributes favouring (1) propagule pressure; (2) dispersal; and (3) establishment and population growth? Using a literature-derived database on 312 stream macroinvertebrate genera occurring at 527 least human-impacted European sites, we quantitatively examined this question. Compared with native genera, genera with invasive invertebrate species (1) tended to reproduce more frequently and to have higher abundances (i.e. higher propagule pressure); (2) had no particular resistance stages to survive during dispersal; and (3) had significantly more ovoviviparity (enabling colonization by a single individual that typically releases viable offspring), larger size and longer life (providing resistance against mortality), food and feeding habits exploiting food resources in streams more effectively, and tended to be more dominant in their communities (all favouring establishment and population growth). Repeating these analyses excluding “native” flying insects (i.e. genera that presumably invaded from glacial refuges unnoticed by biologists), fewer biological attributes were significantly associated with invasiveness. For both data sets (all genera or insects excluded), their affinity to few biological traits (e.g. ovoviviparity, gill respiration) assigned the same 13 of the 19 invasive genera to the top 19 ranks on a gradient of potential invasiveness, together with native genera that risk to become invasive (e.g. Pisidium, Unio), but also with one endangered native genus (Margaritifera). Overall, our data support the idea that invasiveness can be predicted using biological attributes. 相似文献
883.
Cytoplasmic polyadenylation plays a key role in the translational control of mRNAs driving biological processes such as gametogenesis, cell-cycle progression, and synaptic plasticity. What determines the distinct time of polyadenylation and extent of translational control of a given mRNA, however, is poorly understood. The polyadenylation-regulated translation is controlled by the cytoplasmic polyadenylation element (CPE) and its binding protein, CPEB, which can assemble both translational repression or activation complexes. Using a combination of mutagenesis and experimental validation of genome-wide computational predictions, we show that the number and relative position of two elements, the CPE and the Pumilio-binding element, with respect to the polyadenylation signal define a combinatorial code that determines whether an mRNA will be translationally repressed by CPEB, as well as the extent and time of cytoplasmic polyadenylation-dependent translational activation. 相似文献
884.
Helmi K Skraber S Gantzer C Willame R Hoffmann L Cauchie HM 《Applied and environmental microbiology》2008,74(7):2079-2088
Biofilms colonizing surfaces inside drinking water distribution networks may provide a habitat and shelter to pathogenic viruses and parasites. If released from biofilms, these pathogens may disseminate in the water distribution system and cause waterborne diseases. Our study aimed to investigate the interactions of protozoan parasites (Cryptosporidium parvum and Giardia lamblia [oo]cysts) and viruses (vaccinal poliovirus type 1, phiX174, and MS2) with two contrasting biofilms. First, attachment, persistence, and detachment of the protozoan parasites and the viruses were assessed with a drinking water biofilm. This biofilm was allowed to develop inside a rotating annular reactor fed with tap water for 7 months prior to the inoculation. Our results show that viable parasites and infectious viruses attached to the drinking water biofilm within 1 h and persisted within the biofilm. Indeed, infectious viruses were detected in the drinking water biofilm up to 6 days after the inoculation, while viral genome and viable parasites were still detected at day 34, corresponding to the last day of the monitoring period. Since viral genome was detected much longer than infectious particles, our results raise the question of the significance of detecting viral genomes in biofilms. A transfer of viable parasites and viruses from the biofilm to the water phase was observed after the flow velocity was increased but also with a constant laminar flow rate. Similar results regarding parasite and virus attachment and detachment were obtained using a treated wastewater biofilm, suggesting that our observations might be extrapolated to a wide range of environmental biofilms and confirming that biofilms can be considered a potential secondary source of contamination. 相似文献
885.
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887.
Contemporary variations of immune responsiveness during range expansion of two invasive rodents in Senegal
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Christophe Diagne Emmanuelle Gilot‐Fromont Stéphane Cornet Laëtitia Husse Souleymane Doucouré Ambroise Dalecky Khalilou Bâ Mamadou Kane Youssoupha Niang Mamoudou Diallo Aliou Sow Odile Fossati‐Gaschignard Sylvain Piry Emmanuelle Artige Mbacké Sembène Carine Brouat Nathalie Charbonnel 《Oikos》2017,126(3):435-446
Biological invasions provide unique opportunities for studying life history trait changes over contemporary time scales. As spatial spread may be related to changes in parasite communities, several hypotheses (such as the evolution of increased competitive ability (EICA) or EICA‐refined hypotheses) suggest immune changes in invasive species along invasion gradients. Although native hosts may be subject to similar changes in parasite selection pressures, their immune responses have been rarely investigated in invasion contexts. In this study, we evaluated immune variations for invasive house mice Mus musculus domesticus, invasive black rats Rattus rattus and native rodents Mastomys erythroleucus and Mastomys natalensis along well‐characterised invasion gradients in Senegal. We focused on antibody‐mediated (natural antibodies and complement) and inflammatory (haptoglobin) responses. One invasion route was considered for each invasive species, and environmental conditions were recorded. Natural‐antibody mediated responses increased between sites of long‐established invasion and recently invaded sites only in house mice. Both invasive species exhibited higher inflammatory responses at the invasion front than in sites of long‐established invasion. The immune responses of native species did not change with the presence of invasive species. These patterns of immune variations do not support the EICA and EICA refined hypotheses, and they rather suggest a higher risk of exposure to parasites on the invasion front. Altogether, these results provide a first basis to further assess the role of immune changes in invasion success. 相似文献
888.
Correction to “Historical biogeography and phylogeny of the pantropical Psychotrieae alliance (Rubiaceae), with particular emphasis on the Western Indian Ocean Region”
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Sylvain G. Razafimandimbison Kent Kainulainen Niklas Wikström Birgitta Bremer 《American journal of botany》2017,104(10):1596-1596
889.
Mammalian cell culture monitoring using in situ spectroscopy: Is your method really optimised?
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Silvère André Sylvain Lagresle Zahia Hannas Éric Calvosa Ludovic Duponchel 《Biotechnology progress》2017,33(2):308-316
In recent years, as a result of the process analytical technology initiative of the US Food and Drug Administration, many different works have been carried out on direct and in situ monitoring of critical parameters for mammalian cell cultures by Raman spectroscopy and multivariate regression techniques. However, despite interesting results, it cannot be said that the proposed monitoring strategies, which will reduce errors of the regression models and thus confidence limits of the predictions, are really optimized. Hence, the aim of this article is to optimize some critical steps of spectroscopic acquisition and data treatment in order to reach a higher level of accuracy and robustness of bioprocess monitoring. In this way, we propose first an original strategy to assess the most suited Raman acquisition time for the processes involved. In a second part, we demonstrate the importance of the interbatch variability on the accuracy of the predictive models with a particular focus on the optical probes adjustment. Finally, we propose a methodology for the optimization of the spectral variables selection in order to decrease prediction errors of multivariate regressions. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:308–316, 2017 相似文献
890.
Caecilia Charbonnier Sylvain Chagué Frank C. Kolo Victoria B. Duthon Jacques Menetrey 《Computer methods in biomechanics and biomedical engineering》2017,20(14):1571-1579
When estimating knee kinematics from skin markers and stereophotogrammetry, multi-body optimization (MBO) has provided promising results for reducing soft tissue artefacts (STA), but can still be improved. The goal of this study was to assess the performance of MBO with subject-specific knee models at high knee flexion angles (up to 110°) against knee joint kinematics measured by magnetic resonance imaging. Eight subjects were recruited. MBO with subject-specific knee models was more effective in compensating STA compared to no kinematic and spherical constraints, in particular for joint displacements. Moreover, it seems to be more reliable over large ranges of knee flexion angle. The ranges of root mean square errors for knee rotations/displacements were 3.0°–9.2°/1.3–3.5 mm for subject-specific knee models, 6.8°–8.7°/6.0–12.4 mm without kinematic constraint and 7.1°–9.8°/4.9–12.5 mm for spherical constraints. 相似文献