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91.
Nims RW Sykes G Cottrill K Ikonomi P Elmore E 《In vitro cellular & developmental biology. Animal》2010,46(10):811-819
The role of cell authentication in biomedical science has received considerable attention, especially within the past decade.
This quality control attribute is now beginning to be given the emphasis it deserves by granting agencies and by scientific
journals. Short tandem repeat (STR) profiling, one of a few DNA profiling technologies now available, is being proposed for
routine identification (authentication) of human cell lines, stem cells, and tissues. The advantage of this technique over
methods such as isoenzyme analysis, karyotyping, human leukocyte antigen typing, etc., is that STR profiling can establish
identity to the individual level, provided that the appropriate number and types of loci are evaluated. To best employ this
technology, a standardized protocol and a data-driven, quality-controlled, and publically searchable database will be necessary.
This public STR database (currently under development) will enable investigators to rapidly authenticate human-based cultures
to the individual from whom the cells were sourced. Use of similar approaches for non-human animal cells will require developing
other suitable loci sets. While implementing STR analysis on a more routine basis should significantly reduce the frequency
of cell misidentification, additional technologies may be needed as part of an overall authentication paradigm. For instance,
isoenzyme analysis, PCR-based DNA amplification, and sequence-based barcoding methods enable rapid confirmation of a cell
line’s species of origin while screening against cross-contaminations, especially when the cells present are not recognized
by the species-specific STR method. Karyotyping may also be needed as a supporting tool during establishment of an STR database.
Finally, good cell culture practices must always remain a major component of any effort to reduce the frequency of cell misidentification. 相似文献
92.
Slater L Bartlett NW Haas JJ Zhu J Message SD Walton RP Sykes A Dahdaleh S Clarke DL Belvisi MG Kon OM Fujita T Jeffery PK Johnston SL Edwards MR 《PLoS pathogens》2010,6(11):e1001178
The relative roles of the endosomal TLR3/7/8 versus the intracellular RNA helicases RIG-I and MDA5 in viral infection is much debated. We investigated the roles of each pattern recognition receptor in rhinovirus infection using primary bronchial epithelial cells. TLR3 was constitutively expressed; however, RIG-I and MDA5 were inducible by 8-12 h following rhinovirus infection. Bronchial epithelial tissue from normal volunteers challenged with rhinovirus in vivo exhibited low levels of RIG-I and MDA5 that were increased at day 4 post infection. Inhibition of TLR3, RIG-I and MDA5 by siRNA reduced innate cytokine mRNA, and increased rhinovirus replication. Inhibition of TLR3 and TRIF using siRNA reduced rhinovirus induced RNA helicases. Furthermore, IFNAR1 deficient mice exhibited RIG-I and MDA5 induction early during RV1B infection in an interferon independent manner. Hence anti-viral defense within bronchial epithelium requires co-ordinated recognition of rhinovirus infection, initially via TLR3/TRIF and later via inducible RNA helicases. 相似文献
93.
Sykes BD 《Structure (London, England : 1993)》2007,15(7):753-754
Insect flight muscle is capable of very high oscillatory frequencies. In this issue of Structure, De Nicola and colleagues (De Nicola et al., 2007) describe the structure of the Ca2+ binding protein that regulates asynchronous contraction, casting light on the mechanism of stretch activation. 相似文献
94.
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96.
Piero Visconti Michel Bakkenes Robert J. Smith Lucas Joppa Rachel E. Sykes 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2015,370(1681)
Several global strategies for protected area (PA) expansion have been proposed to achieve the Convention on Biological Diversity''s Aichi target 11 as a means to stem biodiversity loss, as required by the Aichi target 12. However, habitat loss outside PAs will continue to affect habitats and species, and PAs may displace human activities into areas that might be even more important for species persistence. Here we measure the expected contribution of PA expansion strategies to Aichi target 12 by estimating the extent of suitable habitat available for all terrestrial mammals, with and without additional protection (the latter giving the counterfactual outcome), under different socio-economic scenarios and consequent land-use change to 2020. We found that expanding PAs to achieve representation targets for ecoregions under a Business-as-usual socio-economic scenario will result in a worse prognosis than doing nothing for more than 50% of the world''s terrestrial mammals. By contrast, targeting protection towards threatened species can increase the suitable habitat available to over 60% of terrestrial mammals. Even in the absence of additional protection, an alternative socio-economic scenario, adopting progressive changes in human consumption, leads to positive outcomes for mammals globally and to the largest improvements for wide-ranging species. 相似文献
97.
Disentangling the effects of land‐use change,climate and CO2 on projected future European habitat types
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98.
Laboratories often repeatedly determine the structure of a given protein under a variety of conditions, mutations, modifications, or in a number of states. This approach can be cumbersome and tedious. Given then a database of structures, identifiers, and corresponding (1)H,(15)N-HSQC NMR spectra for homologous proteins, we investigated whether structural information could be ascertained for a new homolog solely from its (1)H,(15)N-HSQC NMR spectrum. We addressed this question with two different approaches. First, we used a semi-automated approach with the program, ORBplus. ORBplus looks for patterns in the chemical shifts and correlates these commonalities to the explicit property of interest. ORBplus ranks resonances based on consistency of the magnitude and direction of the chemical shifts within the database, and the chemical shift correlation of the unknown protein with the database. ORBplus visualizes the results by a histogram and a vector diagram, and provides residue specific predictions on structural similarities with the database. The second method we used was partial least squares (PLS), which is a multivariate statistical technique used to correlate response and predictor variables. We investigated the ability of these methods to predict the tertiary structure of the contractile regulatory protein troponin C. Troponin C undergoes a closed-to-open conformational change, which is coupled to its function in muscle. We found that both ORBplus and PLS were able to identify patterns in the (1)H,(15)N-HSQC NMR data from different states of troponin C that correlated to its conformation. 相似文献
99.
Hermanrud CE Lucas CL Sykes M Huang CA Wang Z 《Protein expression and purification》2011,76(1):115-120
The anti-murine CD40L monoclonal antibody MR1 has been widely used in immunology research to block the CD40-CD40L interaction for induction of transplantation tolerance and to abrogate autoimmune diseases. The availability of recombinant CD40L with high binding capacity for MR1 would provide a valuable immunologic research tool. In this study, we constructed the single chain murine soluble CD40L monomer, dimer, trimer and successfully expressed them in yeast Pichia pastoris under the control of the alcohol oxidase promoter. The secreted single chain murine soluble CD40L monomers, dimers, and trimers were initially enriched through histidine tag capture by Ni-Sepharose 6 fast flow resin and further purified on a cation exchange resin. Purity reached more than 95% for the monomer and dimer forms and more than 90% for the trimer. Protein yield following purification was 16 mg/L for the monomer and dimer, and 8 mg/L for the trimer. ELISA analysis demonstrated that the CD40L dimers and trimers correctly folded in conformations exposing the MR1 antigenic determinant. 相似文献
100.
Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts 总被引:7,自引:0,他引:7
Pardo SJ Patel MJ Sykes MC Platt MO Boyd NL Sorescu GP Xu M van Loon JJ Wang MD Jo H 《American journal of physiology. Cell physiology》2005,288(6):C1211-C1221