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排序方式: 共有190条查询结果,搜索用时 15 毫秒
81.
Monika Grote Ines Swoboda Richard B. Meagher Rudolf Valenta 《Sexual plant reproduction》1995,8(3):180-186
Double immunogold labeling of profilin and actin was performed on ultrathin sections of in vitro germinated tobacco pollen using different anti-profilin and anti-actin antibodies. Since profilin, besides its role as an actin-binding protein, is known as an allergen, water-free fixation in p-formaldehyde vapor was used. Profilin labeling occurs throughout the cytoplasm of the pollen tube. There is no profilin in the pollen tube wall. Actin reactivity is found in the cytoplasm and extracellularly in the pollen tube wall where three out of four different anti-actin antibodies give a positive signal. This labeling of the pollen tube wall may result from a wall-bound actin, an isoform of actin not yet described or from the presence of a molecule immunologically indistinguishable from actin. 相似文献
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84.
C André Lévesque Henk Brouwer Liliana Cano John P Hamilton Carson Holt Edgar Huitema Sylvain Raffaele Gregg P Robideau Marco Thines Joe Win Marcelo M Zerillo Gordon W Beakes Jeffrey L Boore Dana Busam Bernard Dumas Steve Ferriera Susan I Fuerstenberg Claire MM Gachon Elodie Gaulin Francine Govers Laura Grenville-Briggs Neil Horner Jessica Hostetler Rays HY Jiang Justin Johnson Theerapong Krajaejun Haining Lin Harold JG Meijer Barry Moore Paul Morris Vipaporn Phuntmart Daniela Puiu Jyoti Shetty Jason E Stajich Sucheta Tripathy Stephan Wawra Pieter van West Brett R Whitty Pedro M Coutinho Bernard Henrissat Frank Martin Paul D Thomas Brett M Tyler Ronald P De Vries Sophien Kamoun Mark Yandell Ned Tisserat C Robin Buell 《Genome biology》2010,11(7):1-22
85.
Miranda-Vizuete A Fierro González JC Gahmon G Burghoorn J Navas P Swoboda P 《FEBS letters》2006,580(2):484-490
Thioredoxins are a class of small proteins that play a key role in regulating many cellular redox processes. We report here the characterization of the first member of the thioredoxin family in metazoans that is mainly associated with neurons. The Caenorhabditis elegans gene B0228.5 encodes a thioredoxin (TRX-1) that is expressed in ASJ ciliated sensory neurons, and to some extent also in the posterior-most intestinal cells. TRX-1 is active at reducing protein disulfides in the presence of a heterologous thioredoxin reductase. A mutant worm strain carrying a null allele of the trx-1 gene displays a reproducible decrease in both mean and maximum lifespan when compared to wild-type. The identification and characterization of TRX-1 paves the way to use C. elegans as an in vivo model to study the role of thioredoxins in lifespan and nervous system physiology and pathology. 相似文献
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Miłoszewska J Szaniawska B Trembacz H Gos M Przybyszewska M Swoboda P Małecki M Janik P 《Cell proliferation》2006,39(3):195-203
The density-dependent growth inhibition of non-transformed cells may be associated with inefficient transduction of the proliferative signal from cell adhesion molecules. To verify this concept, the C3H10T1/2 fibroblasts were stably transfected with the gene coding for the fibronectin fragment III/10 (FNIII/10). This resulted in differences in gene's expression between original C3H10T1/2 cells and their FNIII/10 transfectants. No significant differences in growth properties were observed in the original or in the transfected cells. C3H10T1/2 cells and their transfectants, when co-cultured, displayed more cells at confluence than the cells cultured alone. Moreover, co-cultured C3H10T1/2 cells and their transfectants showed elevated levels of phospho-ERK1/2 compared to homogenous cultures. Results obtained indicate that cellular homogeneity is responsible for density-dependent growth inhibition. 相似文献
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Edward L. Vargo Laurianne Leniaud Lois E. Swoboda Sarah E. Diamond Michael D. Weiser Dini M. Miller Anne‐Geneviève Bagnères 《Molecular ecology》2013,22(5):1447-1462
Social insects exhibit remarkable variation in their colony breeding structures, both within and among species. Ecological factors are believed to be important in shaping reproductive traits of social insect colonies, yet there is little information linking specific environmental variables with differences in breeding structure. Subterranean termites (Rhinotermitidae) show exceptional variation in colony breeding structure, differing in the number of reproductives and degree of inbreeding; colonies can be simple families headed by a single pair of monogamous reproductives (king and queen) or they can be extended families headed by multiple inbreeding neotenic reproductives (wingless individuals). Using microsatellite markers, we characterized colony breeding structure and levels of inbreeding in populations over large parts of the range of the subterranean termites Reticulitermes flavipes in the USA and R. grassei in Europe. Combining these new data with previous results on populations of both species, we found that latitude had a strong effect on the proportion of extended‐family colonies in R. flavipes and on levels of inbreeding in both species. We examined the effect of several environmental variables that vary latitudinally; while the degree of inbreeding was greatest in cool, moist habitats in both species, seasonality affected the species differently. Inbreeding in R. flavipes was most strongly associated with climatic variables (mean annual temperature and seasonality), whereas nonclimatic variables, including the availability of wood substrate and soil composition, were important predictors of inbreeding in R. grassei. These results are the first showing that termite breeding structure is shaped by local environmental factors and that species can vary in their responses to these factors. 相似文献
90.
Petra Leidinger Christina Backes Stephanie Deutscher Katja Schmitt Sabine C Mueller Karen Frese Jan Haas Klemens Ruprecht Friedemann Paul Cord St?hler Christoph JG Lang Benjamin Meder Tamas Bartfai Eckart Meese Andreas Keller 《Genome biology》2013,14(7):R78