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81.
Gayathri Babarenda Gamage Carol Boyle Sarah J. McLaren Jake McLaren 《The International Journal of Life Cycle Assessment》2008,13(5):401-411
Background, aims and scope The environmental aspects of companies and their products are becoming more significant in delivering competitive advantage.
Formway Furniture, a designer and manufacturer of office furniture products, is a New Zealand-based company that is committed
to sustainable development. It manufactures two models of the light, intuitive, flexible and environmental (LIFE) office chair:
one with an aluminium base and one with a glass-filled nylon (GFN) base. It was decided to undertake a life cycle assessment
(LCA) study of these two models in order to: (1) determine environmental hotspots in the life cycle of the two chairs (goal
1); (2) compare the life cycle impacts of the two chairs (goal 2); and (3) compare alternative potential waste-management
scenarios (goal 3). The study also included sensitivity analysis with respect to recycled content of aluminium in the product.
Materials and methods The LIFE chair models consist of a mix of metal and plastic components manufactured by selected Formway suppliers according
to design criteria. Hence, the research methodology included determining the specific material composition of the two chair
models and acquisition of manufacturing data from individual suppliers. These data were compiled and used in conjunction with
pre-existing data, specifically from the ecoinvent database purchased in conjunction with the SimaPro7 LCA software, to develop
the life cycle inventory of the two chair models. The life cycle stages included in the study extended from raw-material extraction
through to waste management. Impact assessment was carried out using CML 2 baseline 2000, the methodology developed by Leiden
University’s Institute for Environmental Sciences.
Results This paper presents results for global warming potential (GWP100). The study showed a significant impact contribution from
the raw-material extraction/refinement stage for both chair models; aluminium extraction and refining made the greatest contribution
to GWP100. The comparison of the two LIFE chair models showed that the model with the aluminium base had a higher GWP100 impact
than the model with the GFN base. The waste-management scenario compared the GWP100 result when (1) both chair models were
sent to landfill and (2) steel and aluminium components were recycled with the remainder of the chair sent to landfill. The
results showed that the recycling scenario contributed to a reduced GWP100 result. Since production and processing of aluminium
was found to be significant, a sensitivity analysis was carried out to determine the impact of using aluminium with different
recycled contents (0%, 34% and 100%) in both waste-management scenarios; this showed that increased use of recycled aluminium
was beneficial. The recycling at end-of-life scenarios was modelled using two different end-of-life allocation approaches,
i.e. consequential and attributional, in order to illustrate the variation in results caused by choice of allocation approach.
The results using the consequential approach showed that recycling at end-of-life was beneficial, while use of the attributional
method led to a similar GWP100 as that seen for the landfill scenario.
Discussion The results show that the main hotspot in the life cycle is the raw-material extraction/refinement stage. This can be attributed
to the extraction and processing of aluminium, a material that is energy intensive. The LIFE chair model with the aluminium
base has a higher GWP100 as it contains more aluminium. Sensitivity analysis pertaining to the recycled content of aluminium
showed that use of aluminium with high recycled content was beneficial; this is because production of recycled aluminium is
less energy intensive than production of primary aluminium. The waste-management scenario showed that recycling at end-of-life
resulted in a significantly lower GWP100 than landfilling at end-of-life. However, this result is dependent upon the modelling
approach used for recycling.
Conclusions With respect to goal 1, the study found that the raw-material extraction/refinement stage of the life cycle was a significant
factor for both LIFE chair models. This was largely due to the use of aluminium in the product. For goal 2, it was found that
the LIFE chair model with the aluminium base had a higher GWP100 than the GFN model, again due to the material content of
the two models. Results for goal 3 illustrated that recycling at end-of-life is beneficial when using a system expansion (consequential)
approach to model recycling; if an attributional ‘cut-off’ approach is used to model recycling at end-of-life, there is virtually
no difference in the results between landfilling and recycling. Sensitivity analysis pertaining to the recycled content of
aluminium showed that use of higher recycled contents leads to a lower GWP100 impact.
Recommendation and perspectives Most of the GWP100 impact was contributed during the raw-material extraction/refinement stage of the life cycle; thus, the
overall impact of both LIFE chair models may be reduced through engaging in material choice and supply chain environmental
management with respect to environmental requirements. The study identified aluminium components as a major contributor to
GWP100 for both LIFE chair models and also highlighted the sensitivity of the results to its recycled content. Thus, it is
recommended that the use of aluminium in future product designs be limited unless it is possible to use aluminium with a high
recycled content. With respect to waste management, it was found that a substantial reduction in the GWP100 impact would occur
if the chairs are recycled rather than landfilled, assuming an expanding market for aluminium. Thus, recycling the two LIFE
chair models at end-of-life is highly recommended. 相似文献
82.
Manganese‐ and 1‐methyl‐4‐phenylpyridinium‐induced neurotoxicity display differences in morphological,electrophysiological and genome‐wide alterations: implications for idiopathic Parkinson's disease
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Rajeswara Babu Mythri Narayana Reddy Raghunath Santosh Chandrakant Narwade Mirazkar Dasharatha Rao Pandareesh Kollarkandi Rajesh Sabitha Mohamad Aiyaz Bipin Chand Manas Sule Krittika Ghosh Senthil Kumar Bhagyalakshmi Shankarappa Soundarya Soundararajan Phalguni Anand Alladi Meera Purushottam Narayanappa Gayathri Deepti Dileep Deobagkar Thenkanidiyoor Rao Laxmi Muchukunte Mukunda Srinivas Bharath 《Journal of neurochemistry》2017,143(3):334-358
83.
84.
Nup358 binds to AGO proteins through its SUMO‐interacting motifs and promotes the association of target mRNA with miRISC
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85.
Michael A. Morse Arvind Chaudhry Elizabeth S. Gabitzsch Amy C. Hobeika Takuya Osada Timothy M. Clay Andrea Amalfitano Bruce K. Burnett Gayathri R. Devi David S. Hsu Younong Xu Stephanie Balcaitis Rajesh Dua Susan Nguyen Joseph P. Balint Jr. Frank R. Jones H. Kim Lyerly 《Cancer immunology, immunotherapy : CII》2013,62(8):1293-1301
First-generation, E1-deleted adenovirus subtype 5 (Ad5)-based vectors, although promising platforms for use as cancer vaccines, are impeded in activity by naturally occurring or induced Ad-specific neutralizing antibodies. Ad5-based vectors with deletions of the E1 and the E2b regions (Ad5 [E1-, E2b-]), the latter encoding the DNA polymerase and the pre-terminal protein, by virtue of diminished late phase viral protein expression, were hypothesized to avoid immunological clearance and induce more potent immune responses against the encoded tumor antigen transgene in Ad-immune hosts. Indeed, multiple homologous immunizations with Ad5 [E1-, E2b-]-CEA(6D), encoding the tumor antigen carcinoembryonic antigen (CEA), induced CEA-specific cell-mediated immune (CMI) responses with antitumor activity in mice despite the presence of preexisting or induced Ad5-neutralizing antibody. In the present phase I/II study, cohorts of patients with advanced colorectal cancer were immunized with escalating doses of Ad5 [E1-, E2b-]-CEA(6D). CEA-specific CMI responses were observed despite the presence of preexisting Ad5 immunity in a majority (61.3 %) of patients. Importantly, there was minimal toxicity, and overall patient survival (48 % at 12 months) was similar regardless of preexisting Ad5 neutralizing antibody titers. The results demonstrate that, in cancer patients, the novel Ad5 [E1-, E2b-] gene delivery platform generates significant CMI responses to the tumor antigen CEA in the setting of both naturally acquired and immunization-induced Ad5-specific immunity. 相似文献
86.
Girish Kumar V Surendranathan KP Gayathri Devi DR Belwadi MR 《Indian journal of experimental biology》2005,43(2):187-191
Dietary onion and garlic caused an increase in the level of liver and plasma total lipids in Coturnix coturnix japonica. This increase could be due to the effect of an increased feed intake, bile production, digestion and absorption that in turn caused an increased utilisation of dietary fat, increased transfer of dietary lipids to the liver and/or due to increased lipogenesis as such. However, there was no increase in the muscle lipid content. This effect in the muscle could be due to inhibitory effect of onion or garlic on lipoprotein lipase activity. Further, the changes in the tissue total lipid level in the control group due to change in age and sex were also observed. 相似文献
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88.
Eaazhisai K Jayalakshmi R Gayathri P Anand RP Sumathy K Balaram H Murthy MR 《Journal of molecular biology》2004,335(5):1251-1264
In the absence of the de novo purine nucleotide biosynthetic pathway in parasitic protozoa, purine salvage is of primary importance for parasite survival. Enzymes of the salvage pathway are, therefore, good targets for anti-parasitic drugs. Adenylosuccinate synthetase (AdSS), catalysing the first committed step in the synthesis of AMP from IMP, is a potential target for anti-protozoal chemotherapy. We report here the crystal structure of adenylosuccinate synthetase from the malaria parasite, Plasmodium falciparum, complexed to 6-phosphoryl IMP, GDP, Mg2+ and the aspartate analogue, hadacidin at 2 A resolution. The overall architecture of P. falciparum AdSS (PfAdSS) is similar to the known structures from Escherichia coli, mouse and plants. Differences in substrate interactions seen in this structure provide a plausible explanation for the kinetic differences between PfAdSS and the enzyme from other species. Additional hydrogen bonding interactions of the protein with GDP may account for the ordered binding of substrates to the enzyme. The dimer interface of PfAdSS is also different, with a pronounced excess of positively charged residues. Differences highlighted here provide a basis for the design of species-specific inhibitors of the enzyme. 相似文献
89.
Gurumurthy P Gayathri TN Bhagavathy S Venkatesan P 《Indian journal of experimental biology》2004,42(1):68-73
A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C. 相似文献
90.
Srinivas KS Chandrasekar G Srivastava R Puvanakrishnan R 《Journal of biochemical and biophysical methods》2004,60(1):23-27
In this paper, we describe a method to obtain a relatively pure mitochondrial and microsomal fractions by subcellular fractionation of human hepatoma cell line C3A using sucrose as the hypoosmotic medium. The cells were subjected to osmotic stress with sucrose and homogenized. Osmolarity was then restored to the cells and the organelles were separated by density gradient centrifugation. The protein profiles were examined by SDS-PAGE and the purity was analysed by marker enzymes and Western blotting. Our results indicate a good separation of mitochondrial and microsomal fractions from human hepatoma C3A cells. 相似文献