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Sodium Butyrate Ameliorates l‐Arginine‐Induced Pancreatitis and Associated Fibrosis in Wistar Rat: Role of Inflammation and Nitrosative Stress
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Gayathri Kanika Sabbir Khan Gopabandhu Jena 《Journal of biochemical and molecular toxicology》2015,29(8):349-359
Several reports indicated that histone deacetylases (HDACs) play a crucial role in inflammation and fibrogenesis. Sodium butyrate (SB) is a short‐chain fatty acid having HDAC inhibition potential. The present study aimed to evaluate the protective effect of SB against l ‐arginine (l ‐Arg)‐induced pancreatic fibrosis in Wistar rats. Pancreatic fibrosis was induced by twice intraperitoneal (i.p.) injections of 20% l ‐Arg (250 mg/100 g) at 2‐h interval on day 1, 4, 7, and 10, whereas SB (800 mg/kg/day) was administrated for 10 days. At the end of the study, biochemical estimations, histological alterations, DNA damage, and the expression of various proteins were evaluated. Posttreatment of SB decreased l ‐Arg‐induced oxidative and nitrosative stress, DNA damage, histological alterations, and fibrosis. Interestingly, posttreatment of SB significantly decreased the expression of α‐smooth muscle actin, interleukin‐1β, inducible nitric oxide synthase, and 3‐nitrotyrosine. The present study demonstrated that posttreatment of SB alleviates l ‐Arg‐induced pancreatic damage and fibrosis in rat. 相似文献
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Gayathri Bommakanti Xianghan Lu Michael P. Citron Tariq Ahmad Najar Gwendolyn J. Heidecker Jan ter Meulen Raghavan Varadarajan Xiaoping Liang 《Journal of virology》2012,86(24):13434-13444
The hemagglutinin protein (HA) on the surface of influenza virus is essential for viral entry into the host cells. The HA1 subunit of HA is also the primary target for neutralizing antibodies. The HA2 subunit is less exposed on the virion surface and more conserved than HA1. We have previously designed an HA2-based immunogen derived from the sequence of the H3N2 A/HK/68 virus. In the present study, we report the design of an HA2-based immunogen from the H1N1 subtype (PR/8/34). This immunogen (H1HA0HA6) and its circular permutant (H1HA6) were well folded and provided complete protection against homologous viral challenge. Antisera of immunized mice showed cross-reactivity with HA proteins of different strains and subtypes. Although no neutralization was observable in a conventional neutralization assay, sera of immunized guinea pigs competed with a broadly neutralizing antibody, CR6261, for binding to recombinant Viet/04 HA protein, suggesting that CR6261-like antibodies were elicited by the immunogens. Stem domain immunogens from a seasonal H1N1 strain (A/NC/20/99) and a recent pandemic strain (A/Cal/07/09) provided cross-protection against A/PR/8/34 viral challenge. HA2-containing stem domain immunogens therefore have the potential to provide subtype-specific protection. 相似文献
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Prabhakara S Khedkar S Loganathan RM Chandana S Gowda M Arakere G Seshasayee AS 《Journal of bacteriology》2012,194(14):3727-3728
We report the draft genome sequence of an ST772 Staphylococcus aureus disease isolate carrying staphylococcal cassette chromosome mec (SCCmec) type V from a pyomyositis patient. Our de novo short read assembly is ∼2.8 Mb and encodes a unique Panton-Valentine leukocidin (PVL) phage with structural genes similar to those of φ7247PVL and novel lysogenic genes at the N termini. 相似文献
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Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species 总被引:4,自引:0,他引:4
Panchatcharam M Miriyala S Gayathri VS Suguna L 《Molecular and cellular biochemistry》2006,290(1-2):87-96
Wound healing consists of an orderly progression of events that re-establish the integrity of the damaged tissue. Several
natural products have been shown to accelerate the healing process. The present investigation was undertaken to determine
the role of curcumin on changes in collagen characteristics and antioxidant property during cutaneous wound healing in rats.
Full-thickness excision wounds were made on the back of rat and curcumin was administered topically. The wound tissues removed
on 4th, 8th and 12th day (post-wound) were used to analyse biochemical and pathological changes. Curcumin increased cellular
proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and type III collagen
content of wound tissues. Curcumin treated wounds were found to heal much faster as indicated by improved rates of epithelialisation,
wound contraction and increased tensile strength which were also confirmed by histopathological examinations. Curcumin treatment
was shown to decrease the levels of lipid peroxides (LPs), while the levels of superoxide dismutase (SOD), catalase (CAT),
glutathione peroxidase (GPx), activities were significantly increased exhibiting the antioxidant properties of curcumin in
accelerating wound healing. Better maturation and cross linking of collagen were observed in the curcumin treated rats, by
increased stability of acid-soluble collagen, aldehyde content, shrinkage temperature and tensile strength. The results clearly
substantiate the beneficial effects of the topical application of curcumin in the acceleration of wound healing and its antioxidant
effect.
Both the authors have contributed equally towards this paper. 相似文献
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Haematocrit measurements and plasma protein electrophoresis were carried out in female pigeons (Columba livia) during the breeding cycle. Non-laying birds showed higher (p < 0.001) haematocrit values than those involved in courtship and mating, nest-building, incubation and feeding and brooding. Significant differences in relative percentage of various plasma protein fractions were observed as a function of breeding. Haematocrit could be a useful index to the non-laying status of pigeons like that in chickens, Gallus domesticus. 相似文献
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Gayathri R Gunadharini DN Arunkumar A Senthilkumar K Krishnamoorthy G Banudevi S Vignesh RC Arunakaran J 《Molecular and cellular biochemistry》2009,320(1-2):197-203
Prostate cancer is a leading cause of death among the aging men. Surgical or radiotherapy is effective when the cancer is confined to the prostate gland but once the cancer spreads beyond the pelvis even chemotherapy and hormonal ablation therapy fails in curing this disease. Our previous studies have shown that diallyl disulfide (DADS) induces cell cycle arrest and also induces apoptosis in PC-3 cells. And now the present study is focused to see whether there is an activation of caspase cascade pathway. Hence, in the present study the apoptotic effect of DADS is studied by Western blot analysis of caspase-3, -9, -10 and Bcl-2, Bad, and Bax protein. The Apoptotic cells were assessed by Hoechst 33342 staining with 25 and 40 microM concentrations of DADS for 24 h. The results have shown that DADS at 25 and 40 microM concentrations has induced the activation of caspases. There is a significant increase in the expression of caspases (3, 9, and 10). The proapoptotic protein Bax has significantly increased at 40 microM of DADS treatment and there is significant increase of Bad protein at both the concentration. Bcl-2 protein has significantly decreased in DADS treated cells. Therefore, the present investigation serves as evidence that DADS may be a therapeutic drug in the treatment of prostate cancer. 相似文献
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Xanthomonas T3S Effector XopN Suppresses PAMP-Triggered Immunity and Interacts with a Tomato Atypical Receptor-Like Kinase and TFT1 总被引:1,自引:0,他引:1
Jung-Gun Kim Xinyan Li Julie Anne Roden Kyle W. Taylor Chris D. Aakre Bessie Su Sylvie Lalonde Angela Kirik Yanhui Chen Gayathri Baranage Heather McLane Gregory B. Martin Mary Beth Mudgett 《The Plant cell》2009,21(4):1305-1323
XopN is a virulence factor from Xanthomonas campestris pathovar vesicatoria (Xcv) that is translocated into tomato (Solanum lycopersicum) leaf cells by the pathogen''s type III secretion system. Xcv ΔxopN mutants are impaired in growth and have reduced ability to elicit disease symptoms in susceptible tomato leaves. We show that XopN action in planta reduced pathogen-associated molecular pattern (PAMP)-induced gene expression and callose deposition in host tissue, indicating that XopN suppresses PAMP-triggered immune responses during Xcv infection. XopN is predicted to have irregular, α-helical repeats, suggesting multiple protein–protein interactions in planta. Consistent with this prediction, XopN interacted with the cytosolic domain of a Tomato Atypical Receptor-Like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) in yeast. XopN/TARK1 and XopN/TFT1 interactions were confirmed in planta by bimolecular fluorescence complementation and pull-down analysis. Xcv ΔxopN virulence defects were partially suppressed in transgenic tomato leaves with reduced TARK1 mRNA levels, indicating that TARK1 plays an important role in the outcome of Xcv–tomato interactions. These data provide the basis for a model in which XopN binds to TARK1 to interfere with TARK1-dependent signaling events triggered in response to Xcv infection. 相似文献
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Jung-Hye Lee Yu-Jin Kim Dae-Young Jeong Gayathri Sathiyaraj Rama Krishna Pulla Ju-Sun Shim Jun-Gyo In Deok-Chun Yang 《Molecular biology reports》2010,37(7):3455-3463
Glutamate decarboxylase (GAD) catalyzes the conversion of l-glutamate to γ-aminobutyric acid (GABA). A full-length cDNA encoding GAD (designated as PgGAD) was isolated and characterized from the root of Panax ginseng C. A. Meyer. The length cDNA of PgGAD was 1881 bp and contained a 1491 bp open reading frame (ORF) encoding a glutamate decarboxylase protein of 496 amino acids,
possessing a Ser-X-X-Lys active site, which belongs to the GAD group. The deduced amino acid sequence of the PgGAD was classified in the plant GAD family and has 76–85% high similarity with other plants as like petunia, Arabidopsis, tomato. Secondary structure of PgGAD was predicted by using SOPMA software program. Southern blot analysis of genomic DNA suggests that, there is more than one
copy of the PgGAD gene. The organ specific gene expression pattern also studied in P. ginseng seedlings, in which the stem showed elevated expression than root, leaf, bud and rhizomes. Along with this, we also confirmed
the gene expression of PgGAD under various abiotic stresses like temperature stress, osmotic stress, anoxia, oxidative stress, and mechanical damage.
Temporal analysis of gene expression except exposure of oxidative stress revealed an enhanced expression after each stresses.
The enzyme activity of PgGAD was stimulated to 2-fold under cold stress. 相似文献