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81.
Khripach VA Sviridov OV Priadko AG Litvinovskaia RP Drach SV Matveentsev VD Novik TV Mikhaĭlopulo KI Zhabinskiĭ VN Zavadskaia MI Aver'kova MA Drachenova OA Chashchina NM 《Bioorganicheskaia khimiia》2007,33(3):371-378
Brassinosteroids are a new group of phytohormones that are widely distributed in plants and play an important role in the processes of plant growth and development. Physiological concentrations of brassinosteroids in plants are extremely low, and their analysis in organs and tissues is very difficult. This study is devoted to the chemical aspects of elaboration and to bioanalytical parameters of an immunoenzymatic system for quantitative determination of the phytohormones 24-epicastasterone and 24-epibrassinolide. 相似文献
82.
Natalia Ignatieva Olga Zakharkina Aygun Dadasheva Anatoly Shekhter Alexander Sviridov Valery Lunin 《Journal of biophotonics》2019,12(12)
The aim of this study was to compare between the changes undergone by the dermal collagen framework when heated by IR laser radiation and by traditional means and to reveal the specific features of the dermal matrix modification under moderate IR laser irradiation. Rabbit skin specimens were heated to 50°C, 55°C, 60°C and 65°C in a calorimeter furnace and with a 1.68‐μm fiber Raman laser. The proportion of the degraded collagen macromolecules was determined by differential scanning calorimetry. Changes in the architectonics of the collagen framework were revealed by using standard, phase‐contrast, polarization optical and scanning electron microscopy techniques. The collagen denaturation and dermal matrix amorphization temperature in the case of laser heating proved to be lower by 10°C than that for heating in the calorimeter furnace. The IR laser treatment of the skin was found to cause a specific low‐temperature (45°C‐50°C) transformation of its collagen framework, with some collagen macromolecules remaining intact. The transformation reduces to the splitting of collagen bundles and distortion of the course of collagen fibers. The denaturation of collagen macromolecules in the case of traditional heating takes its course in a threshold manner, so that their pre‐denaturation morphological changes are insignificant. 相似文献
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85.
Huanhuan L. Cui Belinda Guo Benjamin Scicluna Bradley M. Coleman Victoria A. Lawson Laura Ellett Peter J. Meikle Michael Bukrinsky Nigora Mukhamedova Dmitri Sviridov Andrew F. Hill 《The Journal of biological chemistry》2014,289(2):789-802
Conversion of prion protein (PrPC) into a pathological isoform (PrPSc) during prion infection occurs in lipid rafts and is dependent on cholesterol. Here, we show that prion infection increases the abundance of cholesterol transporter, ATP-binding cassette transporter type A1 (ATP-binding cassette transporter type A1), but reduces cholesterol efflux from neuronal cells leading to the accumulation of cellular cholesterol. Increased abundance of ABCA1 in prion disease was confirmed in prion-infected mice. Mechanistically, conversion of PrPC to the pathological isoform led to PrPSc accumulation in rafts, displacement of ABCA1 from rafts and the cell surface, and enhanced internalization of ABCA1. These effects were abolished with reversal of prion infection or by loading cells with cholesterol. Stimulation of ABCA1 expression with liver X receptor agonist or overexpression of heterologous ABCA1 reduced the conversion of prion protein into the pathological form upon infection. These findings demonstrate a reciprocal connection between prion infection and cellular cholesterol metabolism, which plays an important role in the pathogenesis of prion infection in neuronal cells. 相似文献
86.
Skorb EV Andreeva DV Raiski AP Belyasova NA M?hwald H Sviridov DV 《Photochemical & photobiological sciences》2011,10(12):1974-1978
The investigations on the kinetics of photocatalytic inactivation of bacteriophages, lactic bacteria and lysogenic lactic bacteria have shown that the rate of bacterial inactivation is ca. 10 times less than the inactivation of bacteriophages. Titania-assisted photorelease of bacteriophages from lysogenic bacteria proves that photogenerated reactive oxygen species affect the deoxyribonucleic acid (DNA) of bacteria before their deactivation. On this basis a novel photocatalytic method of a prophage induction to the lytic cycle and detection of lysogenic bacteria is proposed. 相似文献
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88.
Demethylation using the epigenetic modifier, 5-azacytidine, increases the efficiency of transient transfection of macrophages 总被引:3,自引:0,他引:3
Escher G Hoang A Georges S Tchoua U El-Osta A Krozowski Z Sviridov D 《Journal of lipid research》2005,46(2):356-365
This study was aimed at developing a method for high-efficiency transient transfection of macrophages. Seven methods were evaluated for transient transfection of murine macrophage RAW 264.7 cells. The highest transfection efficiency was achieved with DEAE-dextran, although the proportion of cells expressing the reporter gene did not exceed 20%. It was subsequently found that the cytomegalovirus plasmid promoter in these cells becomes methylated. When cells were treated with the methylation inhibitor 5-azacytidine, methylation of the plasmid promoter was abolished and a dose-dependent stimulation of reporter gene expression was observed with expression achieved in more than 80% of cells. Treatment of cells with 5-azacytidine also caused increased efficiency of transfection of macrophages with plasmids driven by RSV, SV40, and EF-1alpha promoters and transient transfection of human HepG2 cells. Inhibition of methylation also increased the amount and activity of sterol 27-hydroxylase (CYP27A1) detected in RAW 264.7 cells transfected with a CYP27A1 expression plasmid. Treatment of cells with 5-azacytidine alone did not affect either cholesterol efflux from nontransfected cells or expression of ABCA1 and CYP27A1. However, transfection with CYP27A1 led to a 2- to 4-fold increase of cholesterol efflux. We conclude that treatment with 5-azacytidine can be used for high-efficiency transient transfection of macrophages. 相似文献
89.
Synthesis of methyl 2,4,6-trideoxy-3-O-benzoyl-2,4-di-C-methyl-alpha-L-talohexopyranoside and 2,4,6-trideoxy-2,4-di-C-methyl-L-galactitol, stereoisomers of the C33-C38 fragment of amphothericin B, is described. 相似文献
90.
Z Olsovsky V Olsáková S Chobot V Sviridov 《Journal of hygiene, epidemiology, microbiology, and immunology》1975,19(1):22-29
Mass occurrence of intestinal disorders caused by Yersinia enterocolitica simultaneously in two groups of children of under-school age is reported. The question of the source of infection and the routes of transfer are discussed and the various forms pointed out which Y. e. infection may take in under-school children within the framework of an epidemic occurrence in a group of children. The fact is emphasized that the results of serological examination using the method of indirect haemagglutination may be an important guide for the orientation in the epidemiological situation and, in some cases, also in making etiological diagnosis when the proof of Y. e. by culture presents difficulties. 相似文献