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排序方式: 共有364条查询结果,搜索用时 734 毫秒
41.
Swinburne JE Boursnell M Hill G Pettitt L Allen T Chowdhary B Hasegawa T Kurosawa M Leeb T Mashima S Mickelson JR Raudsepp T Tozaki T Binns M 《Genomics》2006,87(1):1-29
A genetic linkage map of the horse consisting of 742 markers, which comprises a single linkage group for each of the autosomes and the X chromosome, is presented. The map has been generated from two three-generation full-sibling reference families, sired by the same stallion, in which there are 61 individuals in the F2 generation. Each linkage group has been assigned to a chromosome and oriented with reference to markers mapped by fluorescence in situ hybridization. The average interval between markers is 3.7 cM and the linkage groups collectively span 2772 cM. The 742 markers comprise 734 microsatellite and 8 gene-based markers. The utility of the microsatellite markers for comparative mapping has been significantly enhanced by comparing their flanking sequences with the human genome sequence; this enabled conserved segments between human and horse to be identified. The new map provides a valuable resource for genetically mapping traits of interest in the horse. 相似文献
42.
Carbone L Nergadze SG Magnani E Misceo D Francesca Cardone M Roberto R Bertoni L Attolini C Francesca Piras M de Jong P Raudsepp T Chowdhary BP Guérin G Archidiacono N Rocchi M Giulotto E 《Genomics》2006,87(6):777-782
Centromere repositioning (CR) is a recently discovered biological phenomenon consisting of the emergence of a new centromere along a chromosome and the inactivation of the old one. After a CR, the primary constriction and the centromeric function are localized in a new position while the order of physical markers on the chromosome remains unchanged. These events profoundly affect chromosomal architecture. Since horses, asses, and zebras, whose evolutionary divergence is relatively recent, show remarkable morphological similarity and capacity to interbreed despite their chromosomes differing considerably, we investigated the role of CR in the karyotype evolution of the genus Equus. Using appropriate panels of BAC clones in FISH experiments, we compared the centromere position and marker order arrangement among orthologous chromosomes of Burchelli's zebra (Equus burchelli), donkey (Equus asinus), and horse (Equus caballus). Surprisingly, at least eight CRs took place during the evolution of this genus. Even more surprisingly, five cases of CR have occurred in the donkey after its divergence from zebra, that is, in a very short evolutionary time (approximately 1 million years).These findings suggest that in some species the CR phenomenon could have played an important role in karyotype shaping, with potential consequences on population dynamics and speciation. 相似文献
43.
44.
Viral and bacterial diseases of Atlantic cod Gadus morhua, their prophylaxis and treatment: a review 总被引:1,自引:0,他引:1
Samuelsen OB Nerland AH Jørgensen T Schrøder MB Svåsand T Bergh O 《Diseases of aquatic organisms》2006,71(3):239-254
This review summarises the state of knowledge of both viral and bacterial diseases of Atlantic cod Gadus morhua, and their diagnosis, prophylaxis and treatment. The most important losses have been at the larval and juvenile stages, and vibriosis has long been the most important bacterial disease in cod, with Listonella (Vibrio) anguillarum dominant among pathogenic isolates. Vaccination of cod against pathogens such as L. anguillarum and Aeromonas salmonicida clearly demonstrates that the cod immune system possesses an effective memory and appropriate mechanisms sufficient for protection, at least against some diseases. Well-known viruses such as the nodavirus that causes viral encephalopathy and retinopathy (VER), infectious pancreatic necrosis virus (IPNV) and viral haemorrhagic septicaemia virus (VHSV) have been isolated from Atlantic cod and can be a potential problem under intensive rearing conditions. No commercial vaccines against nodavirus are currently available, whereas vaccines against IPNV infections based upon inactivated virus as well as IPNV recombinant antigens are available. A number of investigations of the pharmacokinetic properties of antibacterial agents in cod and their efficacy in treating bacterial infections have been reviewed. 相似文献
45.
Toenshoff ER Kvellestad A Mitchell SO Steinum T Falk K Colquhoun DJ Horn M 《PloS one》2012,7(3):e32696
Epitheliocystis, a disease characterised by cytoplasmic bacterial inclusions (cysts) in the gill and less commonly skin epithelial cells, has been reported in many marine and freshwater fish species and may be associated with mortality. Previously, molecular and ultrastructural analyses have exclusively associated members of the Chlamydiae with such inclusions. Here we investigated a population of farmed Atlantic salmon from the west coast of Norway displaying gill epitheliocystis. Although 'Candidatus Piscichlamydia salmonis', previously reported to be present in such cysts, was detected by PCR in most of the gill samples analysed, this bacterium was found to be a rare member of the gill microbiota, and not associated with the observed cysts as demonstrated by fluorescence in situ hybridization assays. The application of a broad range 16 S rRNA targeted PCR assay instead identified a novel betaproteobacterium as an abundant member of the gill microbiota. Fluorescence in situ hybridization demonstrated that this bacterium, tentatively classified as 'Candidatus Branchiomonas cysticola', was the cyst-forming agent in these samples. While histology and ultrastructure of 'Ca. B. cysticola' cysts revealed forms similar to the reticulate and intermediate bodies described in earlier reports from salmon in seawater, no elementary bodies typical of the chlamydial developmental cycle were observed. In conclusion, this study identified a novel agent of epitheliocystis in sea-farmed Atlantic salmon and demonstrated that these cysts can be caused by bacteria phylogenetically distinct from the Chlamydiae. 相似文献
46.
47.
Knut E. Jørstad Paulo A. Prodöhl Ann-Lisbeth Agnalt Maria Hughes Apostolos P. Apostolidis Alexandros Triantafyllidis Eva Farestveit Tore S. Kristiansen John Mercer Terje Svåsand 《Environmental Biology of Fishes》2004,69(1-4):223-231
The European lobster is distributed throughout the south and western regions of the Norwegian coast. A previous lobster allozyme investigation (1993) in the Tysfjord region, north of the Arctic Circle demonstrated that the lobster population from this region was genetically different from lobster samples collected in other parts of Norway. More detailed investigation including supplementary extensive sampling and additional allozyme, microsatellite and mtDNA analyses are reported here. This investigation supports the genetic distinctness of the Tysfjord population and shows that this is mainly due to a reduction (60–70%) in gene diversity (observed heterozygosities and number of alleles) compared with lobsters from more southern regions. In addition to the Tysfjord region, the comprehensive sampling also included lobsters found in the adjacent Nordfolda fjord system. Genetic analyses provided evidence for significant differences between the lobster populations of Tysfjord and Nordfolda, even though they are separated by a coastal distance of only 142 km. The two populations were also different with regards to several biological characteristics such as body size. The genetic difference between these two geographically close populations is likely to be due to the local hydrological conditions, preventing larval dispersal between the fjord systems. Assessment of lobster abundance in the north-west region suggests that the sub-arctic lobster populations are geographically isolated. 相似文献
48.
49.
Renuka Chaudhary Terje Raudsepp Xin-Yuan Guan Hongen Zhang Bhanu P. Chowdhary 《Mammalian genome》1998,9(1):44-49
Microdissected arm specific paints (ASPs) for human (HSA) chromosomes (Chrs) 2, 5, 6, 16, and 19 were used as probes on pig
(SSC) and horse (ECA) metaphase chromosomes. Regions homologous to individual human arms were delineated in the two species
studied. Of the ten ASPs used, HSA6 and 16 ASPs showed complete synteny conservation of individual arms as single blocks/arms
both in pig and horse. A similar trend was, in general, also observed for HSA19 ASPs. However, contrary to these observations,
synteny conservation of individual arms of HSA2 and HSA5 was not observed in pig and horse. The arm specific painting data,
coupled with the available gene mapping data, showed that, although HSA2 corresponded to two arms/chromosomes each in pig
and horse, the breakpoint of this synteny in humans was not located at the centromere, but at HSA2q13 band. Similarly, arm
specific paints for HSA5 showed that of the two blocks/chromosomes painted in pig and horse, one corresponded to HSA5q13-pter,
the other to HSA5q13-qter. The findings suggest that 5q13 band may also be an evolutionary break point, similar to the one
detected on HSA2q13. The microdissected human arm specific painting probes used in the present work provide more accurate
and refined comparative information on pig and horse chromosomes than that available through the use of human whole chromosome
specific paints.
Received: 1 June 1997 / Accepted: 5 September 1997 相似文献
50.
Sperm head morphology is associated with sperm swimming speed: A comparative study of songbirds using electron microscopy 下载免费PDF全文
Hanna Nyborg Støstad Arild Johnsen Jan Terje Lifjeld Melissah Rowe 《Evolution; international journal of organic evolution》2018,72(9):1918-1932
Sperm exhibit extraordinary levels of morphological diversification across the animal kingdom. In songbirds, sperm have a helically shaped head incorporating a distinct acrosomal membrane or “helical keel,” the form and extent of which varies across species. The functional significance of this helical shape, however, remains unknown. Using scanning electron microscopy, we quantified inter‐ and intraspecific variation in sperm head morphology across 36 songbird species (Passeriformes: Passerida). Using phylogenetic comparative methods, we investigated the relationship between sperm head morphology and both sperm swimming speed and the frequency of extra‐pair young (EPY). We found that species whose sperm had a relatively more pronounced helical form (i.e., long acrosome, short nucleus, wide helical membrane, and a more pronounced waveform along the sperm head “core”) had faster‐swimming sperm. We found no evidence of a relationship between interspecific variation in sperm head morphology and EPY, although we did find that among‐ and within‐male variation in sperm head traits were negatively correlated with EPY. Applying principles of fluid mechanics, we discuss how the helical form of the sperm head may influence swimming speed, and suggest that further studies considering aspects of sperm morphology beyond sperm length are needed to improve our understanding of sperm structure‐function relationships. 相似文献