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981.
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Mateusz Lezyk Carsten Jers Louise Kjaerulff Charlotte H. Gotfredsen Maria D. Mikkelsen J?rn D. Mikkelsen 《PloS one》2016,11(1)
This paper describes the discovery of novel α-L-fucosidases and evaluation of their potential to catalyse the transglycosylation reaction leading to production of fucosylated human milk oligosaccharides. Seven novel α-L-fucosidase-encoding genes were identified by functional screening of a soil-derived metagenome library and expressed in E. coli as recombinant 6xHis-tagged proteins. All seven fucosidases belong to glycosyl hydrolase family 29 (GH 29). Six of the seven α-L-fucosidases were substrate-inhibited, moderately thermostable and most hydrolytically active in the pH range 6–7, when tested with para-nitrophenyl-α-L-fucopyranoside (pNP-Fuc) as the substrate. In contrast, one fucosidase (Mfuc6) exhibited a high pH optimum and an unusual sigmoidal kinetics towards pNP-Fuc substrate. When tested for trans-fucosylation activity using pNP-Fuc as donor, most of the enzymes were able to transfer fucose to pNP-Fuc (self-condensation) or to lactose. With the α-L-fucosidase from Thermotoga maritima and the metagenome-derived Mfuc5, different fucosyllactose variants including the principal fucosylated HMO 2’-fucosyllactose were synthesised in yields of up to ~6.4%. Mfuc5 was able to release fucose from xyloglucan and could also use it as a fucosyl-donor for synthesis of fucosyllactose. This is the first study describing the use of glycosyl hydrolases for the synthesis of genuine fucosylated human milk oligosaccharides. 相似文献
984.
DA/DAPI heteromorphisms in acrocentric chromosomes other than 15 总被引:1,自引:0,他引:1
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986.
W Brandst?dter 《Folia haematologica (Leipzig, Germany : 1928)》1982,109(6):843-848
A report is presented on the results of investigations made in 188 657 prospective blood donors from 1974-1980 within the district of Magdeburg. Methods of investigation and recommendations of the International Society of Blood Transfusion for selecting blood donors are compared. A total of 12337 (= 6.53%) were not admitted as a donor and 1360 blood conserves (0.77%) could not be used for transfusion because of laboratory findings obtained afterwards. Causes are dealt with in detail. The necessity of supervising the health of donors and the existing limitations for it are pointed out. 相似文献
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N V Guliaeva O L Levina I P Levina 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(8):204-206
Male albino rats were exposed to daily emotional painful stress (EPS) for 4 weeks. The arterial blood pressure of the stressed animals increased and the dynamics of the heart rate changed after functional loading (hypokinesis during one or two hours) as compared with the control group. The increase of the heart weight and activation of cytochrome oxidase activity in the cerebral cortex and hippocampus of rats exposed to EPS were also demonstrated. The use of 20% ethyl alcohol instead of drinking water during EPS partially prevented vegetative disturbances and activation of hippocampal cytochrome oxidase and fully prevented the heart hypertrophy and activation of the enzyme in the cortex. Alcoholization resulted in the increased weight of the spleen and brown adipose tissue and thymus involution. A possible mechanism of the antistress action of alcohol linked with normalization of intensified lipid peroxidation under stress is discussed. 相似文献