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61.
In the fission yeast Schizosaccharomyces pombe, interphase microtubules (MTs) position the nucleus [1, 2], which in turn positions the cell-division plane [1, 3]. It is unclear how the spindle orients, with respect to the predetermined division plane, to ensure that the chromosomes are segregated across this plane. It has been proposed that, during prometaphase, the astral MT interaction with the cell cortex aligns the spindle with the cell axis [4] and also participates in a spindle orientation checkpoint (SOC), which delays entry into anaphase as long as the spindle is misaligned [5-7]. Here, we trace the position of the spindle throughout mitosis in a single-cell assay. We find no evidence for the SOC. We show that the spindle is remarkably well aligned with the cell longitudinal axis at the onset of mitosis, by growing along the axis of the adjacent interphase MT. Misalignment of nascent spindles can give rise to anucleate cells when spindle elongation is impaired. We propose a new role for interphase microtubules: through interaction with the spindle pole body, interphase microtubules determine the initial alignment of the spindle in the subsequent cell division. 相似文献
62.
Gunnar ?hlund Per Hedstr?m Sven Norman Catherine L. Hein G?ran Englund 《Proceedings. Biological sciences / The Royal Society》2015,282(1799)
The temperature dependence of predation rates is a key issue for understanding and predicting the responses of ecosystems to climate change. Using a simple mechanistic model, we demonstrate that differences in the relative performances of predator and prey can cause strong threshold effects in the temperature dependence of attack rates. Empirical data on the attack rate of northern pike (Esox lucius) feeding on brown trout (Salmo trutta) confirm this result. Attack rates fell sharply below a threshold temperature of +11°C, which corresponded to a shift in relative performance of pike and brown trout with respect to maximum attack and escape swimming speeds. The average attack speed of pike was an order of magnitude lower than the escape speed of brown trout at 5°C, but approximately equal at temperatures above 11°C. Thresholds in the temperature dependence of ecological rates can create tipping points in the responses of ecosystems to increasing temperatures. Thus, identifying thresholds is crucial when predicting future effects of climate warming. 相似文献
63.
Klaus S. Larsen Andreas Ibrom Claus Beier Sven Jonasson Anders Michelsen 《Biogeochemistry》2007,85(2):201-213
We measured net ecosystem CO2 flux (F
n) and ecosystem respiration (R
E), and estimated gross ecosystem photosynthesis (P
g) by difference, for two years in a temperate heath ecosystem using a chamber method. The exchange rates of carbon were high
and of similar magnitude as for productive forest ecosystems with a net ecosystem carbon gain during the second year of 293 ± 11 g C m−2 year−1 showing that the carbon sink strength of heather-dominated ecosystems may be considerable when C. vulgaris is in the building phase of its life cycle. The estimated gross ecosystem photosynthesis and ecosystem respiration from October
to March was 22% and 30% of annual flux, respectively, suggesting that both cold-season carbon gain and loss were important
in the annual carbon cycle of the ecosystem. Model fit of R
E of a classic, first-order exponential equation related to temperature (second year; R
2 = 0.65) was improved when the P
g rate was incorporated into the model (second year; R
2 = 0.79), suggesting that daytime R
E increased with increasing photosynthesis. Furthermore, the temperature sensitivity of R
E decreased from apparent Q
10 values of 3.3 to 3.9 by the classic equation to a more realistic Q
10 of 2.5 by the modified model. The model introduces R
photo, which describes the part of respiration being tightly coupled to the photosynthetic rate. It makes up 5% of the assimilated
carbon dioxide flux at 0°C and 35% at 20°C implying a high sensitivity of respiration to photosynthesis during summer. The
simple model provides an easily applied, non-intrusive tool for investigating seasonal trends in the relationship between
ecosystem carbon sequestration and respiration. 相似文献
64.
Janson S 《Environmental microbiology》2004,6(10):1102-1106
Some species of the dinoflagellate genus Dinophysis form red tides and are toxin producers with a great environmental impact. The dinoflagellates as a group display high plastid diversity. Several cases indicate that plastids have been replaced. In the case of the genus Dinophysis, the plastids show characteristics of a plastid originating from a cryptophyte. Recent molecular evidence showed that the plastid indeed originates from a cryptophyte, but the source could not be identified to species or genus level. The data presented here show that both a 799 bp region of the psbA gene and 1,221 bp region of the 16S rRNA gene from Dinophysis spp. are identical to the same loci in Teleaulax amphioxeia SCCAP K434. This strongly indicates that the plastid was acquired recently in Dinophysis and may be a so-called kleptoplastid, specifically originating from a species of Teleaulax. 相似文献
65.
Timo Kanzleiter Michaela Rath Sven W. Görgens Jørgen Jensen Daniel S. Tangen Anders J. Kolnes Kristoffer J. Kolnes Sindre Lee Jürgen Eckel Annette Schürmann Kristin Eckardt 《Biochemical and biophysical research communications》2014
The health-promoting effects of regular exercise are well known, and myokines may mediate some of these effects. The small leucine-rich proteoglycan decorin has been described as a myokine for some time. However, its regulation and impact on skeletal muscle has not been investigated in detail. In this study, we report decorin to be differentially expressed and released in response to muscle contraction using different approaches. Decorin is released from contracting human myotubes, and circulating decorin levels are increased in response to acute resistance exercise in humans. Moreover, decorin expression in skeletal muscle is increased in humans and mice after chronic training. Because decorin directly binds myostatin, a potent inhibitor of muscle growth, we investigated a potential function of decorin in the regulation of skeletal muscle growth. In vivo overexpression of decorin in murine skeletal muscle promoted expression of the pro-myogenic factor Mighty, which is negatively regulated by myostatin. We also found Myod1 and follistatin to be increased in response to decorin overexpression. Moreover, muscle-specific ubiquitin ligases atrogin1 and MuRF1, which are involved in atrophic pathways, were reduced by decorin overexpression. In summary, our findings suggest that decorin secreted from myotubes in response to exercise is involved in the regulation of muscle hypertrophy and hence could play a role in exercise-related restructuring processes of skeletal muscle. 相似文献
66.
Dynamics and functional relevance of ammonia-oxidizing archaea in two agricultural soils 总被引:4,自引:0,他引:4
Kristina Schauss reas Focks Sven Leininger Anja Kotzerke Holger Heuer Sören Thiele-Bruhn Shilpi Sharma Berndt-Michael Wilke Michael Matthies Kornelia Smalla Jean Charles Munch Wulf Amelung Martin Kaupenjohann Michael Schloter Christa Schleper 《Environmental microbiology》2009,11(2):446-456
Crucial steps in geochemical cycles are in many cases performed by more than one group of microorganisms, but the significance of this functional redundancy with respect to ecosystem functioning is poorly understood. Ammonia-oxidizing archaea (AOA) and their bacterial counterparts (AOB) are a perfect system to address this question: although performing the same transformation step, they belong to well-separated phylogenetic groups. Using pig manure amended with different concentrations of sulfadiazine (SDZ), an antibiotic that is frequently used in veterinary medicine, it was possible to affect AOB and AOA to different degrees. Addition of manure stimulated growth of AOB in both soils and, interestingly, also growth of AOA was considerably stimulated in one of the soils. The antibiotic treatments decreased the manure effect notably on AOB, whereas AOA were affected to a lower extent. Model calculations concerning the respective proportions of AOA and AOB in ammonia oxidation indicate a substantial contribution of AOA in one of the soils that further increased under the influence of SDZ, hence indicating functional redundancy between AOA and AOB. 相似文献
67.
Carolin R Loescher Tobias Gro?kopf Falguni D Desai Diana Gill Harald Schunck Peter L Croot Christian Schlosser Sven C Neulinger Nicole Pinnow Gaute Lavik Marcel M M Kuypers Julie LaRoche Ruth A Schmitz 《The ISME journal》2014,8(11):2180-2192
Nitrogen fixation, the biological reduction of dinitrogen gas (N2) to ammonium (NH4+), is quantitatively the most important external source of new nitrogen (N) to the open ocean. Classically, the ecological niche of oceanic N2 fixers (diazotrophs) is ascribed to tropical oligotrophic surface waters, often depleted in fixed N, with a diazotrophic community dominated by cyanobacteria. Although this applies for large areas of the ocean, biogeochemical models and phylogenetic studies suggest that the oceanic diazotrophic niche may be much broader than previously considered, resulting in major implications for the global N-budget. Here, we report on the composition, distribution and abundance of nifH, the functional gene marker for N2 fixation. Our results show the presence of eight clades of diazotrophs in the oxygen minimum zone (OMZ) off Peru. Although proteobacterial clades dominated overall, two clusters affiliated to spirochaeta and archaea were identified. N2 fixation was detected within OMZ waters and was stimulated by the addition of organic carbon sources supporting the view that non-phototrophic diazotrophs were actively fixing dinitrogen. The observed co-occurrence of key functional genes for N2 fixation, nitrification, anammox and denitrification suggests that a close spatial coupling of N-input and N-loss processes exists in the OMZ off Peru. The wide distribution of diazotrophs throughout the water column adds to the emerging view that the habitat of marine diazotrophs can be extended to low oxygen/high nitrate areas. Furthermore, our statistical analysis suggests that NO2− and PO43− are the major factors affecting diazotrophic distribution throughout the OMZ. In view of the predicted increase in ocean deoxygenation resulting from global warming, our findings indicate that the importance of OMZs as niches for N2 fixation may increase in the future. 相似文献
68.
The microbial communities inhabiting the mammalian intestinal tract play an important role in diverse aspects of host biology. However, little is known regarding the forces shaping variation in these communities and their influence on host fitness. To shed light on the contributions of host genetics, transmission and geography to diversity in microbial communities between individuals, we performed a survey of intestinal microbial communities in a panel of 121 house mice derived from eight locations across Western Europe using pyrosequencing of the bacterial 16S rRNA gene. The host factors studied included population structure estimated by microsatellite loci and mitochondrial DNA, genetic distance and geography. To determine whether host tissue (mucosa)‐associated communities display properties distinct from those of the lumen, both the caecal mucosa and contents were examined. We identified Bacteroides, Robinsoniella and Helicobacter as the most abundant genera in both the caecal content and mucosa‐associated communities of wild house mice. Overall, we found geography to be the most significant factor explaining patterns of diversity in the intestinal microbiota, with a comparatively weaker influence of host population structure and genetic distance. Furthermore, the influence of host genetic distance was limited to the mucosa communities, consistent with this environment being more intimately coupled to the host. 相似文献
69.
70.
Christine Eulenburg Anna Suling Petra Neuser Alexander Reuss Ulrich Canzler Tanja Fehm Alexander Luyten Martin Hellriegel Linn Woelber Sven Mahner 《PloS one》2016,11(11)
Propensity scoring (PS) is an established tool to account for measured confounding in non-randomized studies. These methods are sensitive to missing values, which are a common problem in observational data. The combination of multiple imputation of missing values and different propensity scoring techniques is addressed in this work. For a sample of lymph node-positive vulvar cancer patients, we re-analyze associations between the application of radiotherapy and disease-related and non-related survival. Inverse-probability-of-treatment-weighting (IPTW) and PS stratification are applied after multiple imputation by chained equation (MICE). Methodological issues are described in detail. Interpretation of the results and methodological limitations are discussed. 相似文献