全文获取类型
收费全文 | 3729篇 |
免费 | 328篇 |
国内免费 | 2篇 |
出版年
2022年 | 17篇 |
2021年 | 53篇 |
2020年 | 50篇 |
2019年 | 48篇 |
2018年 | 43篇 |
2017年 | 52篇 |
2016年 | 94篇 |
2015年 | 148篇 |
2014年 | 178篇 |
2013年 | 224篇 |
2012年 | 295篇 |
2011年 | 288篇 |
2010年 | 180篇 |
2009年 | 163篇 |
2008年 | 219篇 |
2007年 | 233篇 |
2006年 | 222篇 |
2005年 | 231篇 |
2004年 | 236篇 |
2003年 | 200篇 |
2002年 | 206篇 |
2001年 | 33篇 |
2000年 | 20篇 |
1999年 | 44篇 |
1998年 | 52篇 |
1997年 | 45篇 |
1996年 | 33篇 |
1995年 | 28篇 |
1994年 | 23篇 |
1993年 | 42篇 |
1992年 | 27篇 |
1991年 | 21篇 |
1990年 | 25篇 |
1989年 | 28篇 |
1988年 | 20篇 |
1987年 | 17篇 |
1986年 | 19篇 |
1985年 | 15篇 |
1984年 | 18篇 |
1983年 | 17篇 |
1982年 | 17篇 |
1981年 | 16篇 |
1980年 | 9篇 |
1979年 | 15篇 |
1978年 | 12篇 |
1977年 | 12篇 |
1976年 | 10篇 |
1974年 | 10篇 |
1968年 | 6篇 |
1967年 | 5篇 |
排序方式: 共有4059条查询结果,搜索用时 15 毫秒
91.
92.
Lela Jackson Androniqi Qifti Katherine M. Pearce Suzanne Scarlata 《Protein science : a publication of the Protein Society》2020,29(6):1258-1268
Some proteins can serve multiple functions depending on different cellular conditions. An example of a bifunctional protein is inositide‐specific mammalian phospholipase Cβ (PLCβ). PLCβ is activated by G proteins in response to hormones and neurotransmitters to increase intracellular calcium. Recently, alternate cellular function(s) of PLCβ have become uncovered. However, the conditions that allow these different functions to be operative are unclear. Like many mammalian proteins, PLCβ has a conserved catalytic core along with several regulatory domains. These domains modulate the intensity and duration of calcium signals in response to external sensory information, and allow this enzyme to inhibit protein translation in a noncatalytic manner. In this review, we first describe PLCβ's cellular functions and regulation of the switching between these functions, and then discuss the thermodynamic considerations that offer insight into how cells manage multiple and competitive associations allowing them to rapidly shift between functional states. 相似文献
93.
94.
Andrea J. Morash Ben Speers-Roesch Sean Andrew Suzanne Currie 《Journal of fish biology》2021,98(6):1524-1535
Freshwater fish face a variety of spatiotemporal thermal challenges throughout their life. On a broad scale, temperature is an important driver of physiological, behavioural and ecological patterns and ultimately affects populations and overall distribution. These broad patterns are partly underpinned by the small-scale local effects of temperature on individuals within the population. Climate change is increasing the range of daily thermal variation in most freshwater ecosystems, altering behaviour and performance of resident fishes. The aim of this review is understanding how daily thermal variation in temperate rivers affects individual fish physiology, behaviour and overall performance. The following are highlighted in this study: (a) the physical characteristics of rivers that can either buffer or exacerbate thermal variability, (b) the effects of thermal variability on growth and metabolism, (c) the approaches for quantifying thermal variation and thermal stress and (d) how fish may acclimatize or adapt to our changing climate. 相似文献
95.
Jenna Morissette Sula Swart Tyson J. MacCormack Suzanne Currie Andrea J. Morash 《Journal of fish biology》2021,98(6):1585-1589
Typically, laboratory studies on the physiological effects of temperature are conducted using stable acclimation temperatures. Nonetheless, information extrapolated from these studies may not accurately represent wild populations living in thermally variable environments. The aim of this study was to compare the growth rate, metabolism and swimming performance of wild Atlantic salmon exposed to cycling temperatures, 16–21°C, and stable acclimation temperatures, 16, 18.5, 21°C. Growth rate, metabolic rate, swimming performance and anaerobic metabolites did not change among acclimation groups, suggesting that within Atlantic salmon's thermal optimum range, temperature variation has no effect on these physiological properties. 相似文献
96.
97.
Olga Camacho Lydiane Mattio Stefano Draisma Suzanne Fredericq Guillermo Diaz-Pulido 《分类学与生物多样性》2013,11(2):105-130
98.
Suzanne Peyrottes Béatrice Mestre Fabienne Burlina Michael J. Gait 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1443-1448
Abstract We describe a peptide fragment, solid-phase coupling strategy for synthesis of peptide-oligonucleotide conjugates. Model conjugates contained a hydrophobic tetrapeptide, a hydrophobic influenza virus fusion nonapeptide, or a basic octapeptide of the HIV-1 Tat protein coupled to either dT12 or a 16-mer anti-Tat oligodeoxyribonucleotide. Conjugation yields were improved by removal of internucleotide 2-cyanoethyl groups prior to peptide coupling and by use of a C12 spacer between peptide and oligonucleotide. 相似文献
99.
Vincenzo Forgetta Gary Leveque Joana Dias Deborah Grove Robert Lyons Jr. Suzanne Genik Chris Wright Sushmita Singh Nichole Peterson Michael Zianni Jan Kieleczawa Robert Steen Anoja Perera Doug Bintzler Scottie Adams Will Hintz Volker Jacobi Louis Bernier Roger Levesque Ken Dewar 《Journal of biomolecular techniques》2013,24(1):39-49
As part of the DNA Sequencing Research Group of the Association of Biomolecular Resource Facilities, we have tested the reproducibility of the Roche/454 GS-FLX Titanium System at five core facilities. Experience with the Roche/454 system ranged from <10 to >340 sequencing runs performed. All participating sites were supplied with an aliquot of a common DNA preparation and were requested to conduct sequencing at a common loading condition. The evaluation of sequencing yield and accuracy metrics was assessed at a single site. The study was conducted using a laboratory strain of the Dutch elm disease fungus Ophiostoma novo-ulmi strain H327, an ascomycete, vegetatively haploid fungus with an estimated genome size of 30–50 Mb. We show that the Titanium System is reproducible, with some variation detected in loading conditions, sequencing yield, and homopolymer length accuracy. We demonstrate that reads shorter than the theoretical minimum length are of lower overall quality and not simply truncated reads. The O. novo-ulmi H327 genome assembly is 31.8 Mb and is comprised of eight chromosome-length linear scaffolds, a circular mitochondrial conti of 66.4 kb, and a putative 4.2-kb linear plasmid. We estimate that the nuclear genome encodes 8613 protein coding genes, and the mitochondrion encodes 15 genes and 26 tRNAs. 相似文献
100.