全文获取类型
收费全文 | 9809篇 |
免费 | 1043篇 |
国内免费 | 5篇 |
出版年
2021年 | 127篇 |
2020年 | 103篇 |
2019年 | 96篇 |
2018年 | 102篇 |
2017年 | 91篇 |
2016年 | 182篇 |
2015年 | 328篇 |
2014年 | 357篇 |
2013年 | 464篇 |
2012年 | 585篇 |
2011年 | 555篇 |
2010年 | 331篇 |
2009年 | 292篇 |
2008年 | 442篇 |
2007年 | 459篇 |
2006年 | 415篇 |
2005年 | 420篇 |
2004年 | 427篇 |
2003年 | 360篇 |
2002年 | 391篇 |
2001年 | 189篇 |
2000年 | 190篇 |
1999年 | 198篇 |
1998年 | 131篇 |
1997年 | 131篇 |
1996年 | 91篇 |
1995年 | 98篇 |
1994年 | 94篇 |
1993年 | 119篇 |
1992年 | 133篇 |
1991年 | 155篇 |
1990年 | 152篇 |
1989年 | 169篇 |
1988年 | 147篇 |
1987年 | 145篇 |
1986年 | 132篇 |
1985年 | 130篇 |
1984年 | 120篇 |
1983年 | 103篇 |
1982年 | 133篇 |
1981年 | 135篇 |
1980年 | 107篇 |
1979年 | 120篇 |
1978年 | 81篇 |
1977年 | 75篇 |
1976年 | 72篇 |
1975年 | 64篇 |
1974年 | 67篇 |
1973年 | 62篇 |
1972年 | 75篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
11.
12.
13.
14.
15.
16.
17.
Neil E. Klepeis Suzanne C. Hughes Rufus D. Edwards Tracy Allen Michael Johnson Zohir Chowdhury Kirk R. Smith Marie Boman-Davis John Bellettiere Melbourne F. Hovell 《PloS one》2013,8(8)
Interventions are needed to protect the health of children who live with smokers. We pilot-tested a real-time intervention for promoting behavior change in homes that reduces second hand tobacco smoke (SHS) levels. The intervention uses a monitor and feedback system to provide immediate auditory and visual signals triggered at defined thresholds of fine particle concentration. Dynamic graphs of real-time particle levels are also shown on a computer screen. We experimentally evaluated the system, field-tested it in homes with smokers, and conducted focus groups to obtain general opinions. Laboratory tests of the monitor demonstrated SHS sensitivity, stability, precision equivalent to at least 1 µg/m3, and low noise. A linear relationship (R2 = 0.98) was observed between the monitor and average SHS mass concentrations up to 150 µg/m3. Focus groups and interviews with intervention participants showed in-home use to be acceptable and feasible. The intervention was evaluated in 3 homes with combined baseline and intervention periods lasting 9 to 15 full days. Two families modified their behavior by opening windows or doors, smoking outdoors, or smoking less. We observed evidence of lower SHS levels in these homes. The remaining household voiced reluctance to changing their smoking activity and did not exhibit lower SHS levels in main smoking areas or clear behavior change; however, family members expressed receptivity to smoking outdoors. This study established the feasibility of the real-time intervention, laying the groundwork for controlled trials with larger sample sizes. Visual and auditory cues may prompt family members to take immediate action to reduce SHS levels. Dynamic graphs of SHS levels may help families make decisions about specific mitigation approaches. 相似文献
18.
19.
20.
Peroxisomal membrane proteins (PMPs) from the Swiss-Webster mouse are analyzed and compared to those of rats and humans using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. A purification procedure for fresh mouse, rat, or human biopsy liver which enriches peroxisomal/mitochondrial marker enzyme ratios over 100-fold is characterized. When analyzed by SDS-PAGE, membranes of purified liver peroxisomes are shown to contain the same complement of 145-, 70-, 55-, 36-, and 22-kDa PMPs in rats, mice, and humans. A rabbit polyclonal antibody raised against mouse peroxisomal membranes demonstrates immunoreactivity to 145- and 70-kDa proteins in fresh liver homogenates from all three species and in control or Zellweger syndrome fibroblasts from humans. Human autopsy or placental tissues which were refrigerated before analysis exhibited 105-, 55-, and 36-kDa peptides which may be derived from the 145- and 70-kDa peptides. Such conversions, if related to degradation, may explain difficulties in purifying peroxisomes from human autopsy specimens. Variable amounts of the 55-kDa peptide also occurred in mouse adrenal and lung, and the conversion of higher to lower molecular weight PMPs could not be demonstrated by in vitro incubation of mouse liver. Further definition of the structure and variability of mammalian PMPs should be helpful in understanding polyenzymopathies such as Zellweger syndrome. 相似文献