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991.
992.
Grégoire Moutardier Sompert Gereva Suzanne C. Mills Mehdi Adjeroud Ricardo Beldade Jayven Ham Rocky Kaku Pascal Dumas 《PloS one》2015,10(9)
Outbreaks of the corallivorous crown-of-thorns seastar Acanthaster planci (COTS) represent one of the greatest disturbances to coral reef ecosystems in the Indo-Pacific, affecting not only coral reefs but also the coastal communities which rely on their resources. While injection approaches are increasingly used in an attempt to control COTS densities, most of them display severe drawbacks including logistical challenges, high residual environmental impacts or low cost-effectiveness. We tested a new alternative control method based upon acidic injections of cheap, 100% natural products. We investigated the lethal doses, intra- and inter-specific disease transmission and immune responses of COTS when injected with fresh lime juice (extracted from local Citrus arantifolia) and white spirit vinegar. High COTS mortality was achieved with small volumes: 10–20 ml per seastar induced death in 89%/97% of injected specimens after an average 34.3 h/29.8 h for lime juice and vinegar respectively. Highest efficiency was reached for both solutions with double shots of (2 × 10 ml) in two different areas on the body: 100% mortality occurred within 12–24 h, which is similar or faster compared with other current injection methods. Multiple immune measures suggested that death was very likely caused by pH stress from the acidic solutions rather than a bacterial infection. Contagion to either conspecifics or a variety of other reef species was not observed, even at COTS densities 15 times higher than the highest naturally reported. 10 to 20 l lime juice/vinegar could kill up to a thousand COTS at a cost of less than 0.05 USD per specimen; no permits or special handling procedures are required. We conclude that injections of lime juice and vinegar offer great advantages when compared to current best practises and constitute a cheap and natural option for all reefs affected by COTS. 相似文献
993.
David H. Wright Bruce D. Patterson Greg M. Mikkelson Alan Cutler Wirt Atmar 《Oecologia》1997,113(1):1-20
We present a broad comparative assessment of nested subsets in species composition among ecological communities. We assembled
presence-absence data from a broad range of taxa, geographic regions, and spatial scales; and subjected this collection of
datasets to common analyses, including a variety of metrics for measuring nestedness and null hypotheses against which to
evaluate them. Here we identify ecological patterns in the prevalence and strength of nested subset structure, and assess
differences and biases among the available methodologies. In all, we compiled 279 presence-absence matrices, of which 163
do not overlap in their coverage of species and sites. The survey includes studies on vertebrates, arthropods, mollusks, plants,
and other taxa; from north temperate, tropical, and south temperate latitudes. Our results were as follows. Statistically
significant nestedness was common. Assemblages from landbridge archipelagos were strongly nested, and immigration experiments
were least nested. This adds further empirical support to the hypothesis that extinction plays a major role in producing nested
structure. Nestedness was positively correlated with the ratio of the areas of the largest and smallest sites, suggesting
that the range in area of sites affects nestedness. Taxonomic differences in nestedness were weak. Higher taxonomic levels
showed stronger nesting than their constituent lower taxa. We observed no effect of distance of isolation on nestedness; nor
any effects of latitude. With regard to methodology, the metrics Nc and Ut yielded similar results, although Nc proved slightly more flexible in use, and deals differently with tied sites. Similarities also exist in the behavior of N0 (“N”) and Up, and between N1 and Ua. Standardized nestedness metrics were mostly insensitive to matrix size, and were useful in comparative analyses among presence-absence
matrices. Most metrics were affected by the proportion of presences in the matrix. All analyses of nestedness, therefore,
should test for bias due to matrix fill. We suggest that the factors controlling nested subset structure can be thought of
as four filters that species pass to occur at a site: a sampling filter, a distance filter, a habitat filter, and an area
filter – and three constraints on community homogeneity: evolutionary history, recent history, and spatial variation in the
environment. The scale of examination can also have important effects on the degree of nestedness observed.
Received: 13 September 1996 / Accepted: 16 September 1997 相似文献
994.
D J Cutler 《Journal of theoretical biology》1978,73(2):329-345
Current attitudes on the compartment concept, as applied in pharmacokinetics, are discussed. The concept of a relative steady state is introduced, and shown to provide the basis for a definition of a compartment. The physical significance of the compartment parameters is discussed and a method of testing compartment models by direct sampling is described. 相似文献
995.
Avian oviductal secretions contain an exoATPase which has been purified up to 900-fold. Antibodies to this fraction bind to several cell types and to membrane fractions solubilized by selected detergents. The experiments indicate that a related antigen is present in plasma membranes and that exoATPase is released by a shedding process. 相似文献
996.
Drepanopus pectinatus, the most numerous species of Copepod in the Kerguelen Archipelago completes 4 generations a year. The succession of generations follows the hydrological seasons. Each generation may be characterized by the cephalothorax length of the developmental stages C1 to C6. The mean lengths of stages C2 to C6 are shown to be significantly different (P > 0.05) between seasons (i.e. between generations). The size-classes have a unimodal distribution from stages C1 to C4. For copepodite C5 and adults, bimodal histograms reflect the differentiation of males and females.A reverse trend of the development pattern of lengths is demonstrated in this subantarctic species: a decrease with decreasing temperatures (6° to 2°) from April to September, and an increase with the rising temperatures (2° to 7°) from October to February.Two populations of females are distinguishable in spring and summer, by their small (0.950–1.299 mm length of cephalothorax, 55%) or large (1.300–1.800 mm, 45%) size, respectively.The differences in mean length cannot be explained by their relation to the mean temperature alone. Pigment content is suspected to play a role. 相似文献
997.
Adrian J. Cutler Mohammed Saleem Hong Wang 《In vitro cellular & developmental biology. Plant》1991,27(3):104-111
Summary Cereal leaf protoplasts are extremely difficult to culture (recalcitrant) in vitro. There have been few reports of division
and the protoplasts typically exhibit excessive enlargement and vacuolization with reduced cell wall deposition. Inasmuch
as leaf base explants are capable of callus formation in vitro, protoplasts derived from this tissue must have lost the ability
to divide as a consequence of changes induced by the wall-digestion process. We review evidence suggesting that the inhibition
of mitosis in these protoplasts is a consequence of a cascade of events initiated at the plasma membrane. The enzyme treatment
necessary for wall removal triggers membrane depolarization and other changes that can lead to the initiation of lipid peroxidation
and oxidative stress. Mitotically inactive cereal leaf protoplasts are unable to mount a protective response to these degradative
processes. Consequently, the resulting membrane perturbations and permeabilization give rise to secondary effects on the cytoskeleton
and the cell wall. These effects include reduced or absent microtubules as well as reduced and uneven wall deposition. Such
abnormalities are observed in cereal leaf protoplasts and are sufficient to account for recalcitrance because the occurrence
of mitosis is strongly dependent on a normal cell wall and cytoskeleton.
This paper is NRCC number 32475. 相似文献
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