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排序方式: 共有200条查询结果,搜索用时 15 毫秒
191.
Anne-Marie Carey Kirsty Hacking Nichola Picken Suvi Honkanen Sharon Kelly Dariusz M. Niedzwiedzki Robert E. Blankenship Yuuki Shimizu Zheng-Yu Wang-Otomo Richard J. Cogdell 《BBA》2014
This study systematically investigated the different types of LH2 produced by Allochromatium (Alc.) vinosum, a photosynthetic purple sulphur bacterium, in response to variations in growth conditions. Three different spectral forms of LH2 were isolated and purified, the B800-820, B800-840 and B800-850 LH2 types, all of which exhibit an unusual split 800 peak in their low temperature absorption spectra. However, it is likely that more forms are also present. Relatively more B800-820 and B800-840 are produced under low light conditions, while relatively more B800-850 is produced under high light conditions. Polypeptide compositions of the three different LH2 types were determined by a combination of HPLC and TOF/MS. The B800-820, B800-840 and B800-850 LH2 types all have a heterogeneous polypeptide composition, containing multiple types of both α and β polypeptides, and differ in their precise polypeptide composition. They all have a mixed carotenoid composition, containing carotenoids of the spirilloxanthin series. In all cases the most abundant carotenoid is rhodopin; however, there is a shift towards carotenoids with a higher conjugation number in LH2 complexes produced under low light conditions. CD spectroscopy, together with the polypeptide analysis, demonstrates that these Alc. vinosum LH2 complexes are more closely related to the LH2 complex from Phs. molischianum than they are to the LH2 complexes from Rps. acidophila. 相似文献
192.
The collection efficiency and sample retention of a batch-type wetted wall bioaerosol sampling cyclone (BWWC) were experimentally
characterized. The BWWC is designed to sample air at 400 l/min and concentrate the particles into 12 ml of water. Aerosol
is transported into a cylindrically-shaped axial flow cyclone through a tangential slot and the particles are impacted on
the inner wall, which is wetted by air shear acting on a liquid pool at the base of the cyclone. The retention of collected
particles and the aerosol collection efficiency of the BWWC were evaluated with polystyrene latex beads (PSL), sodium fluorescein/oleic
acid droplets, and Bacillus atrophaeus (aka BG) spores. The retention of particles was determined by adding hydrosol directly into the device, running the BWWC
for a pre-set period of time, and then determining the amount of particulate matter recovered relative to the initial amount.
For 1-μm diameter PSL, 90% of the particles were recoverable from the cyclone body immediately after their introduction; however,
only 10% were retained in the collection liquid after 8 h of operation. The aerosol sampling efficiency was determined by
comparing the amount of particulate matter collected in the liquid with that collected by a reference filter. The collection
efficiency was 50–60% for 1- and 3-μm polystyrene (PSL) particles, and 1.5% for 10-μm oleic acid particles. The efficiency
for 3-μm oleic acid droplets was 35%. Explanations are provided for the difference between liquid and solid particle behavior,
and for the low efficiency for the large liquid particles. The collection efficiency for single spore BG was slightly lower
than that for 1-μm PSL. 相似文献
193.
Sanna Trnblom Sara Nisula Suvi T Vaara Meri Poukkanen Sture Andersson Ville Pettil Eero Pesonen 《Innate immunity》2021,27(2):192
We hypothesised that plasma concentrations of biomarkers of neutrophil activation and pro-inflammatory cytokines differ according to the phase of rapidly evolving sepsis. In an observational study, we measured heparin-binding protein (HBP), myeloperoxidase (MPO), IL-6 and IL-8 in 167 sepsis patients on intensive care unit admission. We prospectively used the emergence of the first sepsis-associated organ dysfunction (OD) as a surrogate for the sepsis phase. Fifty-five patients (of 167, 33%) developed the first OD > 1 h before, 74 (44%) within ± 1 h, and 38 (23%) > 1 h after intensive care unit admission. HBP and MPO were elevated at a median of 12 h before the first OD, remained high up to 24 h, and were not associated with sepsis phase. IL-6 and IL-8 rose and declined rapidly close to OD emergence. Elevation of neutrophil activation markers HBP and MPO was an early event in the evolution of sepsis, lasting beyond the subsidence of the pro-inflammatory cytokine reaction. Thus, as sepsis biomarkers, HBP and MPO were not as prone as IL-6 and IL-8 to the effect of sample timing. 相似文献
194.
Nadja Verspagen Suvi Ikonen Marjo Saastamoinen Erik van Bergen 《Proceedings. Biological sciences / The Royal Society》2020,287(1941)
Variation in environmental conditions during development can lead to changes in life-history traits with long-lasting effects. Here, we study how variation in temperature and host plant (i.e. the consequences of potential maternal oviposition choices) affects a suite of life-history traits in pre-diapause larvae of the Glanville fritillary butterfly. We focus on offspring survival, larval growth rates and relative fat reserves, and pay specific attention to intraspecific variation in the responses (G × E × E). Globally, thermal performance and survival curves varied between diets of two host plants, suggesting that host modifies the temperature impact, or vice versa. Additionally, we show that the relative fat content has a host-dependent, discontinuous response to developmental temperature. This implies that a potential switch in resource allocation, from more investment in growth at lower temperatures to storage at higher temperatures, is dependent on the larval diet. Interestingly, a large proportion of the variance in larval performance is explained by differences among families, or interactions with this variable. Finally, we demonstrate that these family-specific responses to the host plant remain largely consistent across thermal environments. Together, the results of our study underscore the importance of paying attention to intraspecific trait variation in the field of evolutionary ecology. 相似文献
195.
Correct repair of DNA double-strand breaks (DSBs) is critical for maintaining genome stability. Whereas gene conversion (GC)-mediated repair is mostly error-free, repair by break-induced replication (BIR) is associated with non-reciprocal translocations and loss of heterozygosity. We have previously shown that a Recombination Execution Checkpoint (REC) mediates this competition by preventing the BIR pathway from acting on DSBs that can be repaired by GC. Here, we asked if the REC can also determine whether the ends that are engaged in a GC-compatible configuration belong to the same break, since repair involving ends from different breaks will produce potentially deleterious translocations. We report that the kinetics of repair are markedly delayed when the two DSB ends that participate in GC belong to different DSBs (termed Trans) compared to the case when both DSB ends come from the same break (Cis). However, repair in Trans still occurs by GC rather than BIR, and the overall efficiency of repair is comparable. Hence, the REC is not sensitive to the “origin” of the DSB ends. When the homologous ends for GC are in Trans, the delay in repair appears to reflect their tethering to sequences on the other side of the DSB that themselves recombine with other genomic locations with which they share sequence homology. These data support previous observations that the two ends of a DSB are usually tethered to each other and that this tethering facilitates both ends encountering the same donor sequence. We also found that the presence of homeologous/repetitive sequences in the vicinity of a DSB can distract the DSB end from finding its bona fide homologous donor, and that inhibition of GC by such homeologous sequences is markedly increased upon deleting Sgs1 but not Msh6. 相似文献
196.
197.
Matti O. Ruuskanen Guilhem Sommeria-Klein Aki S. Havulinna Teemu J. Niiranen Leo Lahti 《Environmental microbiology》2021,23(5):2374-2388
Microbial communities exhibit spatial structure at different scales, due to constant interactions with their environment and dispersal limitation. While this spatial structure is often considered in studies focusing on free-living environmental communities, it has received less attention in the context of host-associated microbial communities or microbiota. The wider adoption of methods accounting for spatial variation in these communities will help to address open questions in basic microbial ecology as well as realize the full potential of microbiome-aided medicine. Here, we first overview known factors affecting the composition of microbiota across diverse host types and at different scales, with a focus on the human gut as one of the most actively studied microbiota. We outline a number of topical open questions in the field related to spatial variation and patterns. We then review the existing methodology for the spatial modelling of microbiota. We suggest that methodology from related fields, such as systems biology and macro-organismal ecology, could be adapted to obtain more accurate models of spatial structure. We further posit that methodological developments in the spatial modelling and analysis of microbiota could in turn broadly benefit theoretical and applied ecology and contribute to the development of novel industrial and clinical applications. 相似文献
198.
Jori O. Ruuskanen Jussi O. T. Sipilä Päivi Rautava Ville Kytö 《Chronobiology international》2018,35(8):1168-1174
Stroke occurrence shows strong correlations with sleep disorders and even subtle sleep disturbances have been shown to affect ischemic stroke (IS) occurrence. Chronobiology also exerts effects, like the morning surge in IS occurrence. Lunar cycles have also been shown to affect sleep and other physiological processes, but studies on moon phases and its possible association with occurrence of stroke are rare and nonconclusive. Therefore, we studied the effects of moon phases on stroke hospitalizations and in-hospital mortality nationwide in Finland in 2004–2014. All patients aged ≥18 years with IS or intracerebral hemorrhage (ICH) as primary discharge diagnosis were included. Daily number of admissions was treated as a response variable while moon phase, year and astronomical season were independent variables in Poisson regression modeling. We found no association between moon phases and stroke occurrence. The overall occurrence rates did not vary between different moon phases for IS or ICH (p = 0.61 or higher). There were no differences between moon phases in daily admission rates among men, women, young and old patients for any of the stroke subtypes. There was no difference in in-hospital mortality with regard to moon phase for IS or ICH overall (p = 0.19 or higher), nor in subgroup analyses. There were no significant interactions between moon phase and astronomical season for stroke occurrence or in-hospital mortality. To conclude, in this over a decade-long nationwide study including a total of 46 million person years of follow-up, we found no association between moon phases and occurrence or in-hospital mortality rates of IS or intracerebral hemorrhage. 相似文献
199.
200.
Sillman J. Uusitalo V. Ruuskanen V. Ojala L. Kahiluoto H. Soukka R. Ahola J. 《The International Journal of Life Cycle Assessment》2020,25(11):2190-2203
The International Journal of Life Cycle Assessment - Renewable energy produced from wind turbines and solar photovoltaics (PV) has rapidly increased its share in global energy markets. At the same... 相似文献