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71.
Susumu Mitsuhashi Kazuko Saito Satonori Kurashige Nobuo Yamaguchi 《Molecular and cellular biochemistry》1978,20(3):131-147
Summary In the studies of experimental salmonellosis, immunization of mice with a live vaccine SER of S. enteritidis was found to be effective against further infection with virulent S. enteritidis 116-54. Macrophages obtained from the peritoneal cavity, subcutaneous tissue or liver of immunized mice inhibited intracellular growth of bacteria and resisted cell degeneration caused by engulfment of virulent 116-54 bacteria. This immunity was called cellular immunity.We discovered by chance in 1961 a transfer agent of immunity (TA) from the culture fluid of immunized macrophages. This agent is RNA in nature and can be extracted from the spleen, peritoneal exudate cells or the lymph node of immunized animals and is called immune (i) RNA. We could demonstrate antibody activity in macrophages treated in vitro or in vivo with iRNA by the immune adherence hemagglutination technique.Cellular immunity against tumor cells could be transferred in vitro or in vivo to lymphocytes through iRNA prepared from the spleen cells of syngeneic, allogeneic and xenogeneic animals immunized with the tumor cells.We prepared iRNA against antigens capable of inducing humoral antibody production in animals, i.e., RBCs, bacterial toxin, bacterial flagella and hapten-protein conjugates. Serum antibody was not demonstrated in recipient animals of iRNAs by single or repeated injections of these agents. However, in these animals an increase in the number of specific antibody-carrying cells was found as rosette-formers. It was found further that prior injection of iRNA could induce immunologic memory and produced a high titer of humoral antibody after a boosting stimulation with a small dose of the corresponding antigen. The required interval between the first iRNA and the second antigenic stimulation, and the minimal effective doses of iRNA and antigen are described.We studied the interaction of iRNA with either T- or B-cells and with both cells using adoptive transfer system, athymic nude mice and neonatally thymectomized (NT) mice. Immune RNAs against T-dependent and T-independent antigens could not induce the proliferation of antibody-carrying cells in cyclophosphamide-treated (B-cell depleted) mice. But these agents could induce the proliferation of rosette-formers, implying that iRNAs can replace some role of T-cells even against T-dependent antigens. B-cells can be directly activated by treatment with iRNA against both T-dependent and T-independent antigens, and they differentiated into rosette-formers.Passive transfers of iRNA were successful in establishing immunity against infection with S. enteritidis, or immunity to Salmonella flagella, RBCs and hapten-protein conjugates. The ability of iRNA to confer a secondary response of antibody formation is serially and passively transmissible in recipient animals. These facts suggest the presence of some mechanism that is responsible for the amplification of antigenic stimulation in the immune response. The RNA-dependent RNA polymerase and RNA-dependent DNA polymerase are presented and their role in the immune response is discussed. 相似文献
72.
Hisao Matsui Mayumi Kasao Susumu Imamura 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1978,145(2):231-236
A method is described for the determination of urinary hippuric acid by high-performance liquid chromatography. The method used ethyl acetate extraction for partial clean-up of the urine. The separation was carried out on a reversed-phase column using 20% methanol in 0.01 M aqueous potassium phosphate containing 0.5% acetic acid as a mobile phase. The column effluent was monitored with a UV detector at 254 nm. Hippuric acid was separated from other normal urine constituents in less than 10 min. Metabolites of xylene and styrene did not interfere with the assay. Analytical recoveries from urine were excellent and peak height and concentration were linearly related. 相似文献
73.
Growth Regulation of Dark-grown Dwarf Barley Coleoptile by the Endogenous IAA Content 总被引:1,自引:0,他引:1
Growth curves of dark-grown coleoptiles of 11 isogenic coleoptilardwarf strains of barley (Hordeum vulagare L. cv. Akashinriki:uzu, 5, 77, 97, 105, 125, 131, 133, 136, 145 and 148) were simulatedwith a logistic equation and the endogenous IAA contents ofthe barley strains were determined. Growth analysis of the dwarfbarley coleoptiles revealed that the final coleoptile lengthwas correlated with the growth rate on the 2nd day after germination(r=0.897), when the growth rate was about maximum. The endogenousIAA Content of the barley strains, measured fluorometrically,indicated that on the 2nd day, the dwarf strains contained lessendogenous IAA than the normal Strain. The IAA content on the2nd day was correlated to the growth rate on the 2nd day (r=0.907,except for Strain 145) and the final coleoptile length (r=0.933,except for strains 77 and 145). The correlation, however, wasnot significant on the 3rd day. These results suggested thatthe dwarfism of the dark-grown coleoptiles of the barley Strainsexamined is primarily controlled by the endogenous IAA content.
1 Present address: Department of Biology, Faculty of Science,Osaka City University, Osaka 558, Japan. (Received February 1, 1982; Accepted April 13, 1982) 相似文献
74.
Michio Takido Susumu Kitanaka Shushichi Takahashi Tomohisa Tanaka 《Phytochemistry》1982,21(2):425-427
Two new yellow pigments, germitosone and methylgermitorosone, were isolated from the seedling of Cassia torosa. The structures of these substances were established as 3,7 dimethyl - 6 - methoxy - 1 - oxo - 2,3,8,9 - tetrahydroxy - 1,2,3,4 - tetrahydroanthracene and 6,9 dimethoxy - 3,7 - dimethyl - 1 - oxo - 2,3,8 - trihydroxy - 1,2,3,4 - tetrahydroanthracene respectively. 相似文献
75.
Susumu Terabayashi 《Journal of plant research》1981,94(2):141-157
The floral vasculature in three allied genera,Plagiorhegma, Jeffersoria andAchyls is investigated, and the results are compared with those ofEpimedium andVancouveria which are related closely toPlagiorhegma andJeffersonia.
The vasculature in the receptacle ofPlagiorhegma andJeffersonia is similar, but that ofAchlys is much simpler. Slightly different trace patterns are observed in the sepals ofPlagiorhegma andJeffersonia. InJeffersonia, the 3-trace condition leaving 2 or 3 gaps is most frequently observed, but inPlagiorhegma traces of a double nature leaving a single gap are more frequent. The traces to the innermost sepals, petals and stamens
are usually of a double nature leaving a single gap in both genera. Regular division and fusion are not observed in the receptacular
stele. The vascular differentiation between sepals and petals is more advanced inPlagiorhegma andJeffersonia than inEpimedium andVancouveria. InAchlys, the traces are all staminal and single throughout their course.
Two parts recognized in the pistils ofPlagiorhegma, Jeffersonia andAchlys are traversed by independent vasculature. The comparisons of pistil morphology including vasculature ofPlagiorhegma, Jeffersonia, Achlys, Epimedium andVancouveria lead to the interpretation that the pistils are based on the same morphological plan. The probable evolutionary trend in
pistil is then suggested in these five genera. 相似文献
76.
Mark J. Ottenbreit Susumu Inoue Ward D. Peterson James C. Lin 《Bioelectromagnetics》1981,2(3):203-215
Human marrow cells were irradiated with 2450-MHz CW microwaves in a fluid-filled waveguide irradiation system. Cell exposure was conducted by placing a marrow cell suspension in 20-μl glass microcapillary tubes that were positioned in the exposure chamber, and irradiated at power densities from 31 to 1,000 mW/cm2 (with corresponding specific absorption rates of 62 to 2,000 mW/g) for 15 minutes. The temperature of the sample was maintained at a fixed point. Sham-irradiated (SI) and microwave-irradiated (MWI) cells were cultured in a methylcellulose culture system for neutrophil colony proliferation. There was no reduction in neutrophil colony number on days 6–7 or 12–14 in cells exposed at 31 or 62 mW/cm2, but as the power density was increased to 1,000 mW/cm2, there was a reduction in colony number of MWI cells compared with SI cells. The microwave interaction with the human neutrophil colony-forming cells was apparently not related to temperature rise, or to the state of cell cycle, and was irreversible. 相似文献
77.
Shuhichi Takahashi Susumu Kitanaka Michio Takido Ushino Sankawa Shoji Shibata 《Phytochemistry》1977,16(7):999-1002
From seedlings of Cassia torosa four dimeric hydroanthracenes have been isolated. Two, a pair of atropisomeric dimers consisting of two molecules o 相似文献
78.
The potential use of antibodies that selectively recognize either X-bearing or Y-bearing sperm is self-evident. Thus our attention was directed to the fact that under optimal conditions, H-Y antibody lyses 50% of mouse spermatozoa. Accordingly, we asked whether expression of H-Y antigen is haploid in spermatozoa from XY male mice heterozygous for the autosomal dominantSxr gene, for if H-Y expression were haploid, H-Y antibody would be expected to kill 75% of spermatozoa derived from these XY,Sxr/- males. However, maximal lysis remained at the 50% level, which indicates that haploid expression of H-Y antigen and the potential immunoselection of Y-(or X-) bearing spermatozoa are unlikely. 相似文献
79.
Wen-Chang Chang Sei-itsu Murota Susumu Tsurufuji 《Prostaglandins & other lipid mediators》1977,13(1):17-24
A main product (PI) transformed from arachidonic acid in carrageenin-induced granuloma in rats was structurally analysed. PI was converted to a more polar substance by the reduction with sodium borohydride. On the basis of the data on gas chromatography-mass spectrometry, its structure was identified with the hemiacetal derivative of 8-(1-hydroxy-3-oxopropyl)-9,12-heptadecadienoic acid (Thromboxane B2) 相似文献
80.
The levels of free amino acids in the cerebral cortex of acute and chronic uremic rats were examined. Amino acids significantly elevated were aspartate, glutamine, glycine, histidine, ornithine, phenylalanine, phosphoethanolamine and taurine, whereas 1-methyl histidine and 3-methyl histidine were specifically detected in uremic rats. Glutamate, arginine and carnosine disclosed a significant reduction. There was no change in the concentrations of γ-aminobutyrate and alanine. The above findings were essentially identical in both acute and chronic uremia. It was conjectured that these changes of amino acid levels in the brain might participate in the progress of uremic encephalopathy. 相似文献