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601.
C M Chadwick P R Collodi M Sussman 《Differentiation; research in biological diversity》1985,29(2):101-108
During fruiting-body construction by Dictyostelium discoideum, the formation and subsequent maintenance of the multicellular assembly involve two stage-specific cohesive systems that are acquired sequentially and are distinguishable on serological and genetic grounds. We demonstrated that both systems, termed aggregation related (AR) and postaggregation related (PAR), can function in vitro. Ghosts prepared from cells of the wild-type and of a cohesion-defective mutant that were harvested during growth and at aggregation and postaggregative stages of fruiting-body construction exhibited the same cohesive properties as the cells from which they were derived. Membrane fragments prepared from the ghosts by mechanical disruption retained these cohesive properties. 相似文献
602.
Cultivation of Dictyostelium discoideum in axenic medium 总被引:12,自引:0,他引:12
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604.
Mark L. Sussman John B. Hays Theodore A.G. Smith 《Archives of biochemistry and biophysics》1977,182(1):134-137
Low concentrations of sodium dodecyl sulfate (0.015%) and sodium deoxycholate (0.33%) completely inhibit phosphorylation of β-galactosides by the lactose phosphotransferase system of Staphylococcus aureus. Inhibition is reversible, even after prolonged detergent treatment. Phosphorylation of methyl-α-glucoside by the same preparations is only slightly inhibited by 0.015% dodecyl sulfate. The membrane-bound component, Enzyme IFlac, is not solubilized by 0.015% dodecyl sulfate, nor is its ability to bind [14C]lactose affected. The results are consistent with hypotheses of selective binding of anionic detergent to Enzyme IIlac or to Factor IIIlac, the detergent serving in the latter case as a membrane analog. 相似文献
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606.
Stefano Benvegnù Diego Franciotta Josh Sussman Angela Bachi Elisabetta Zardini Paola Torreri Cedric Govaerts Salvatore Pizzo Giuseppe Legname 《PloS one》2009,4(6)
Doppel protein (Dpl) is a paralog of the cellular form of the prion protein (PrPC), together sharing common structural and biochemical properties. Unlike PrPC, which is abundantly expressed throughout the central nervous system (CNS), Dpl protein expression is not detectable in the CNS. Interestingly, its ectopic expression in the brain elicits neurodegeneration in transgenic mice. Here, by combining native isoelectric focusing plus non-denaturing polyacrylamide gel electrophoresis and mass spectrometry analysis, we identified two Dpl binding partners: rat alpha-1-inhibitor-3 (α1I3) and, by sequence homology, alpha-2-macroglobulin (α2M), two known plasma metalloproteinase inhibitors. Biochemical investigations excluded the direct interaction of PrPC with either α1I3 or α2M. Nevertheless, enzyme-linked immunosorbent assays and surface plasmon resonance experiments revealed a high affinity binding occurring between PrPC and Dpl. In light of these findings, we suggest a mechanism for Dpl-induced neurodegeneration in mice expressing Dpl ectopically in the brain, linked to a withdrawal of natural inhibitors of metalloproteinase such as α2M. Interestingly, α2M has been proven to be a susceptibility factor in Alzheimer''s disease, and as our findings imply, it may also play a relevant role in other neurodegenerative disorders, including prion diseases. 相似文献
607.