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451.
Relationship of hypoxic ventilatory response to exercise performance on Mount Everest 总被引:6,自引:0,他引:6
452.
Lahiri S.; Rumsey W. L.; Wilson D. F.; Iturriaga R. 《Journal of applied physiology》1993,75(3):1035-1043
453.
We studied the responses of the ganglioglomerular nerve (GGN) efferents to brief periods of hypoxia and hypercapnia and to several levels of steady-state arterial PO2 and PCO2 and to intravascular injection of cyanide in thirteen anesthetized cats. The cats breathed spontaneously. A branch of the GGN which was cut close to the carotid body was divided into several filaments, and the activity of each filament was tested until clean and identifiable action potentials were obtained. The GGN efferent activity, breath-by-breath inspiratory volume, tracheal PO2 and PCO2 and arterial blood pressure were recorded simultaneously. We found that the GGN contained spontaneously active fibers which showed a range of responses to the respiratory stimuli. Fifty-eight percent of the filaments with dominant cardiovascular rhythm showed the least response to blood gas stimuli. Forty-two percent showed clear responses to hypoxia and hypercapnia. These responses developed slowly with the onset of the stimulus but decreased promptly with the withdrawal of the stimulus. These GGN efferents were also promptly stimulated by sodium cyanide. The steady-state response curve to hypoxia was hyperbolic and to hypercapnia it was linear. Some of these fibers showed stronger respiratory rhythms than others. The responses of these GGN efferents were associated with the respiratory responses to hypoxia and hypercapnia. For the same respiratory drive, however, the steady-state hypoxic stimulus elicited a greater GGN response than did hypercapnia. 相似文献
454.
Shibojyoti Lahiri Na Sun Achim Buck Axel Imhof Axel Walch 《Expert review of proteomics》2016,13(3):275-284
Histone post-translational modifications (PTMs), histone variants and enzymes responsible for the incorporation or the removal of the PTMs are being increasingly associated with human disease. Combinations of histone PTMs and the specific incorporation of variants contribute to the establishment of cellular identity and hence are potential markers that could be exploited in disease diagnostics and prognostics and therapy response prediction. Due to the scarcity of suitable antibodies and the pre-requirement of tissue homogenization for more advanced analytical techniques, comprehensive information regarding the spatial distribution of these factors at the tissue level has been lacking. MALDI imaging mass spectrometry provides an ideal platform to measure histone PTMs and variants from tissues while maintaining the information about their spatial distribution. Discussed in this review are the relevance of histones in the context of human disease and the contribution of MALDI imaging mass spectrometry in measuring histones in situ. 相似文献
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457.
Best linear unbiased prediction is well known for its wide rangeof applications including small area estimation. While the theoryis well established for mixed linear models and under normalityof the error and mixing distributions, the literature is sparsefor nonlinear mixed models under nonnormality of the error distributionor of the mixing distributions. We develop a resampling-basedunified approach for predicting mixed effects under a generalizedmixed model set-up. Second-order-accurate nonnegative estimatorsof mean squared prediction errors are also developed. Giventhe parametric model, the proposed methodology automaticallyproduces estimators of the small area parameters and their meansquared prediction errors, without requiring explicit analyticalexpressions for the mean squared prediction errors. 相似文献
458.
Partial purification of the dihydropyridine receptor from rat skeletal muscle demonstrated mainly a 60 kDa band in SDS-polyacrylamide gel. An antibody raised against that protein behaved as a calcium channel agonist viz. Bay K8644. The affinity purified antibody, when added to cultured heart cells, increased the beat rate 40-80% depending on the titer of the antiserum. The antibody also woke up the beats of the cells previously blocked with the channel antagonist, nifedipine. Immunoblot analysis indicated that the receptor of this antibody in heart cell membrane is also a 60 kDa protein. 相似文献
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460.
Sushmita Pradhan 《Journal of biomolecular structure & dynamics》2013,31(13):3341-3353
Nucleotide excision repair (NER) in higher organisms repair massive DNA abrasions caused by ultraviolet rays, and various mutagens, where Xeroderma pigmentosum group A (XPA) protein is known to be involved in damage recognition step. Any mutations in XPA cause classical Xeroderma pigmentosum disease. The extent to which XPA is required in the NER is still unclear. Here, we present the comparative study on the structural and conformational changes in globular DNA binding domain of XPA98–210 in DNA bound and DNA free state. Atomistic molecular dynamics simulation was carried out for both XPA98–210 systems using AMBER force fields. We observed that XPA98–210 in presence of damaged DNA exhibited more structural changes compared to XPA98–210 in its free form. When XPA is in contact with DNA, we found marked stability of the complex due to the formation of characteristic longer antiparallel β-sheets consisting mainly lysine residues. 相似文献