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51.
Preet Kamal Kaur Neha Tripathi Jayesh Desale Soumya Neelagiri Shailendra Yadav Prasad V. Bharatam Sushma Singh 《PloS one》2016,11(3)
Ribose-5-phosphate isomerase B from Leishmania donovani (LdRpiB) is one of the potential drug targets against visceral leishmaniasis. In the present study, we have targeted several conserved amino acids for mutational analysis (i.e. Cys69, His11, His102, His138, Asp45, Tyr46, Pro47 and Glu149) to gain crucial insights into their role in substrate binding, catalysis and conformational stability of the enzyme. All the eight LdRpiB variants were cloned, sequenced, expressed and purified. C69S, H102N, D45N and E149A mutants exhibited complete loss of enzyme activity indicating that they are indispensable for the enzyme activity. Kinetic parameters were altered in case of H138N, H11N and P47A variants; however Y46F exhibited similar kinetic behaviour as wild type. All the mutants except H138N exhibited altered protein structure as determined by CD and fluorescence spectral analysis. This data was supported by the atomic level details of the conformational changes and substrate binding using molecular dynamic simulations. LdRpiB also exhibited activity with D-form of various aldose substrates in the order of D-ribose > D-talose > D-allose > D-arabinose. Our study provides insights for better understanding of substrate enzyme interactions which can rationalize the process of drug design against parasite RpiB. 相似文献
52.
Stefan Andersson Wayne M. Geissler Sushma Patel Ling Wu 《The Journal of steroid biochemistry and molecular biology》1995,53(1-6):37-39
The enzyme 17β-hydroxysteroid dehydrogenase (17β-HSD) catalyzes the 17β-oxidation/reduction of C18- and C19-steroids in a variety of tissues. Three human genes encoding isozymes of 17β-HSD, designated 17β-HSD types 1, 2 and 3 have been cloned. 17β-HSD type 1 (also referred to as estradiol 17β-dehydrogenase) catalyzes the conversion of estrone to estradiol, primarily in the ovary and placenta. The 17β-HSD type 2 is expressed to high levels in the liver, secretory endometrium and placenta. The type 2 isozyme catalyzes the oxidation of androgens and estrogens equally efficiently. Also, the enzyme possesses 20-HSD activity demonstrated by its ability to convert 20-dihydro-progesterone to progesterone. Testicular 17β-HSD type 3 catalyzes the conversion of androstenedione to testosterone, dehydroepiandrosterone to 5-androstenediol and estrone to estradiol. The 17β-HSD3 gene is mutated in male pseudohermaphrodites with the genetic disease 17β-HSD deficiency. 相似文献
53.
James R. Priest Kazutoyo Osoegawa Nebil Mohammed Vivek Nanda Ramendra Kundu Kathleen Schultz Edward J. Lammer Santhosh Girirajan Todd Scheetz Daryl Waggott Francois Haddad Sushma Reddy Daniel Bernstein Trudy Burns Jeffrey D. Steimle Xinan H. Yang Ivan P. Moskowitz Matthew Hurles Richard P. Lifton Debbie Nickerson Michael Bamshad Evan E. Eichler Seema Mital Val Sheffield Thomas Quertermous Bruce D. Gelb Michael Portman Euan A. Ashley 《PLoS genetics》2016,12(4)
54.
Binay K. Singh Dwijesh C. Mishra Sushma Yadav Supriya Ambawat Era Vaidya Kishor U Tribhuvan Arun Kumar Sujith Kumar Sanjeev Kumar K. K. Chaturvedi Reema Rani Prashant Yadav Anil Rai P. K. Rai V. V. Singh Dhiraj Singh 《Journal of plant biochemistry and biotechnology.》2016,25(4):410-420
Brassica juncea is an economically important oilseed crop worldwide. It has limited genomic resources at present. We generated 47,962,057 expressed sequence reads which were assembled into 45,280 unigenes. A total of 4108 SSR loci (≥10 bp) were identified in these unigenes. Trinucleotide was the most frequent repeat unit (59.91 %) followed by di- (38.66 %), tetra - (0.71 %), hexa - (0.49 %) and pentanucleotide repeats (0.24 %). Primers were designed for 2863 SSR loci among which 460 were selected for primer synthesis. A total of 339 loci amplified successfully of which 134 (39.5 %) exhibited polymorphism among six B. juncea genotypes with PIC values ranging from 0.18 to 0.81. Further, 25 polymorphic SSRs were used for analysis of genetic variability in 25 genotypes of Brassicas and their wild relatives. Two to five alleles with PIC values 0.22–0.66 were detected at these loci. The dendrogram grouped the genotypes according to their known pedigree/systematic position. 相似文献
55.
Sushma Rishi V. K. Agarwal G. R. Anand S. P. Agarwal D. S. Balaine P. K. Dwaraknath 《Biochemical genetics》1981,19(9-10):987-996
Serum guanase levels of Indian Zebu (Hariana) cattle and its crosses with different exotic levels of temperate breeds of dairy cattle were estimated in 527 animals, and their associations with economic characters were determined. The overall mean value of serum guanase level was 13.46±0.45 IU/ml of serum. An inverse relationship between the concentration of the enzyme and the frequency of cattle falling within a definite range was observed, as over 75% of the animals possessed 1 to 20 IU/ml of serum guanase. An analysis of variance revealed significant differences (P<0.01) in enzyme levels among different genetic groups under study. Hariana (Zebu) purebreds had significantly higher enzyme levels (19.60±1.85) than any of the crossbred groups, whose enzyme levels ranged from 8.99±1.41 in Red Dane F1 half-breds to 14.30±1.11 in Brown Swiss F1 half-breds. The correlation and regression coefficients were significantly positive for reproductive traits such as age at first service and age at first calving, and significantly negative for production traits such as lactation yield and lactation length. These results suggest that selection of dairy cattle based on low serum guanase levels might result in correlated improvements in dairy cattle productivity. 相似文献
56.
Sushma R. Dhingra S. Duraiswami Narender Kumar Lakshmi G. Kumari 《Journal of biosciences》1989,14(2):79-90
Studies on the characterization of inhibin and inhibin-like factors have depended for the most part on the classicalin vitro pituitary cell culture assay. A major drawback with this assay is the turn-around time which is in the order of two weeks
and consequently slows down purification efforts. The 24 h bioassay for inhibin has been found to be sufficiently sensitive
and also statistically valid. Unfortunately, based as it is on a secondary response, ambiguities arise in interpreting the
results. By including a parallel assay in which the mice are primed with human menopausal gonadotropin rather than human chorionic
gonadotropin, it was possible to device the coupled bioassay. This enables distinguishing inhibin-like factors acting to suppress
pituitary follicle stimulating hormone output from those acting at the level of gonads. In this study the coupled assay for
inhibin has been compared with the classical pituitary cell culture assay in order to assess its biological and statistical
validity. The data validates the bioassay on both the above counts and when considered in conjunction with the short turn-around
time suggests that this assay can be highly useful in studies on isolation of inhibin from various sources. 相似文献
57.
Tarandeep Kaur Hilal A. Bhat Anuj Raina Sushma Koul Dhiraj Vyas 《Acta Physiologiae Plantarum》2013,35(8):2501-2511
Perennial pepperweed (Lepidium latifolium Linn.) is a preferred ‘phytofood’ that is available for the longest period of a year in Ladakh. Present study was undertaken to identify the mechanism of redox homeostasis and understand factors responsible for its biochemical superiority during low temperatures. Results reveal that despite the stressful environment at higher altitude, the cellular conditions are more reducing for this plant. The reducing environment is maintained by significant induction of GSH rather than changes in its oxidation state, which changes the redox potential by 12 mV. Lower ratio of NADP+/NADPH and induction of new antioxidative isozymes at Leh (3,505 m) suggest crucial role of redox regulation in adaptation. These new proteins have higher thiol content and could provide an efficient redox sensing mechanism in Lepidium latifolium that respond through GSH/NADPH redox buffers. In vitro feeding experiment suggested that GSH plays an important role in induction of antioxidant enzymes, which may not be the direct consequence of H2O2 accumulation. It needs to be further investigated whether its responsive redox metabolism has some role in its invasive growth in riparian plains of America. 相似文献
58.
Sushma Hosur Subbarao Ramesh Kumar P. Kumara Honnavalli N. 《Primates; journal of primatology》2022,63(2):149-149
Primates - In the original publication of the article, the second author’s name was wrongly published. The correct name is given in this correction. 相似文献
59.
60.
Prabhakara S Khedkar S Loganathan RM Chandana S Gowda M Arakere G Seshasayee AS 《Journal of bacteriology》2012,194(14):3727-3728
We report the draft genome sequence of an ST772 Staphylococcus aureus disease isolate carrying staphylococcal cassette chromosome mec (SCCmec) type V from a pyomyositis patient. Our de novo short read assembly is ∼2.8 Mb and encodes a unique Panton-Valentine leukocidin (PVL) phage with structural genes similar to those of φ7247PVL and novel lysogenic genes at the N termini. 相似文献