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91.
The pH dependence of the activity, aggregation, and secondary structure of Artocarpus hirsuta lectin was studied using intrinsic and extrinsic fluorescence, light scattering, and circular dichroism. The lectin is more stable in the neutral and acidic than in the alkaline pH range, which is also reflected in the binding constants of the lectin to methyl alpha-galactopyranoside (me alpha-gal). The aggregation of the protein due to heat denaturation is prevented at both extremes of pH. The binding of hydrophobic dye to the lectin takes place at pH 1-2, which increases with increasing temperature. The exposure of hydrophobic patches at pH 1 is reversible. The secondary structure of the lectin is intact in the pH range of 1-8 and is distorted above pH 9. Aggregation of the protein due to heat denaturation is also prevented in the presence of guanidine hydrochloride (GdnHCl). 相似文献
92.
Shripad N. Surwase Sushama A. Patil Shekhar B. Jadhav Jyoti P. Jadhav 《Microbial biotechnology》2012,5(6):731-737
l ‐DOPA (3,4‐dihydroxyphenyl‐l ‐alanine) is an extensively used drug for the treatment of Parkinson's disease. In the present study, optimization of nutritional parameters influencing l ‐DOPA production was attempted using the response surface methodology (RSM) from Brevundimonas sp. SGJ. A Plackett–Burman design was used for screening of critical components, while further optimization was carried out using the Box–Behnken design. The optimized levels of factors predicted by the model were pH 5.02, 1.549 g l?1 tryptone, 4.207 g l?1 l ‐tyrosine and 0.0369 g l?1 CuSO4, which resulted in highest l ‐DOPA yield of 3.359 g l?1. The optimization of medium using RSM resulted in a 8.355‐fold increase in the yield of l ‐DOPA. The anova showed a significant R2 value (0.9667), model F‐value (29.068) and probability (0.001), with insignificant lack of fit. The highest tyrosinase activity observed was 2471 U mg?1 at the 18th hour of the incubation period with dry cell weight of 0.711 g l?1. l ‐DOPA production was confirmed by HPTLC, HPLC and GC‐MS analysis. Thus, Brevundimonas sp. SGJ has the potential to be a new source for the production of l ‐DOPA. 相似文献
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Sushama M Gaikwad Madhura M Gurjar M Islam Khan 《European journal of biochemistry》2002,269(5):1413-1417
Unfolding, inactivation and dissociation of the lectin from Artocarpus hirsuta seeds were studied by chemical (guanidine hydrochloride, GdnHCl) and thermal denaturation. Conformational transitions were monitored by intrinsic fluorescence and circular dichroism. The gradual red shift in the emission maxima of the native protein from 335 to 356 nm, change in the ellipticity at 218 nm and simultaneous decrease in the sugar binding activity were observed with increasing concentration of GdnHCl in the pH range between 4.0 and 9.0. The unfolding and inactivation by GdnHCl were partially reversible. Gel filtration of the lectin in presence of 1-6 m GdnHCl showed that the protein dissociates reversibly into partially unfolded dimer and then irreversibly into unfolded inactive monomer. Thermal denaturation was irreversible. The lectin loses activity rapidly above 45 degrees C. The exposure of hydrophobic patches, distorted secondary structure and formation of insoluble aggregates of the thermally inactivated protein probably leads to the irreversible denaturation. 相似文献
97.
The effect of carnitine administration on levels of lipid peroxide and activities of superoxide dismutase and catalase was
studied in rats administered isoproterenol to induce myocardial infarction. Levels of fatty acid were lower in rats pretreated
with carnitine at the peak period and given isoproterenol than the levels in isoproterenoltreated control rats. Lipid peroxides
were decreased in the heart at peak infarction in carnitine-treated rats compared to the levels in isoproterenol-treated controls.
Activities of superoxide dismutase and catalase showed no change in carnitine-treated animals given isoproterenol compared
to those in normal control rats, while they decreased in animals treated with isoproterenol alone. 相似文献