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931.
932.
Beginning in the early 1990s, the balsam fir sawfly (Neodiprion abietis) became a significant defoliating insect of precommercially thinned balsam fir (Abies balsamea (L.) Mill.) stands in western Newfoundland, Canada. In 1997, a nucleopolyhedrovirus (NeabNPV) was isolated from the balsam
fir sawfly and, as no control measures were then available, NeabNPV was developed for the biological control of balsam fir
sawfly. In order to register NeabNPV for operational use under the Canadian Pest Control Products Act, research was carried
out in a number of areas including NeabNPV field efficacy, non-target organism toxicology, balsam fir sawfly ecology and impact
on balsam fir trees, and NeabNPV genome sequencing and analysis. As part of the field efficacy trials, approximately 22 500
hectares of balsam fir sawfly-infested forest were aerially treated with NeabNPV between 2000 and 2005. NeabNPV was found
to be safe, efficacious, and economical for the suppression of balsam fir sawfly outbreak populations. Conditional registration
for the NeabNPV-based product, Abietiv™, was received from the Pest Management Regulatory Agency (Health Canada) in April
2006. In July 2006, Abietiv was applied by spray airplanes to 15 000 ha of balsam fir sawfly-infested forest in western Newfoundland
in an operational control program.
相似文献
933.
To achieve higher level expression of Interferon α2b (IFN-α2b) in methylotrophic yeast (Pichia pastoris), a cDNA fragment coding for the mature IFN-α2b was designed and synthesized based on the synonymous codon bias of P. pastoris and optimized G+C content. The synthetic IFN-α2b was inserted into the secreted expression vector pPICZαA, and then integrated
into P. pastoris GS115 genome by electroporation. Multi-copy integrants in the Mut+ recombinant P. pastoris strain were screened by high concentrations of Zeocin. 120 hours culturing allowed expression of the IFN-α2b transformant
up to 810 mg/L as detected by SDS-PAGE and quantitative methods. In addition, Western blot analysis showed that the recombinant
proteins had immunogenicity. The significant antiviral activity of the recombinant IFN-α2b protein was verified by WISH/ VSV
system, which was 3.3×105 IU/mL.
Foundation items: The National ‘973’ Basic Research Program (2002CB111302); The National Natural Science Foundation of China
(30370807) 相似文献
934.
Xiao-Jie Li Rie Uenishi Saiki Hase Huanan Liao Tee Kok Keng Shigeru Kusagawa Yutaka Takebe 《中国病毒学》2007,22(6):426-433
The Asia-Pacific region is a home to 60% of the population in the world and to approximately one quarter of people with HIV/AIDS.
Close to a million of people has been infected and a half million people died of AIDS annually in Asia, becoming the second
largest epicenter of global AIDS epidemic. Molecular epidemiology has been useful tool to track a course of HIV spread. In-depth
knowledge from the studies on molecular epidemiology elucidates the dynamics of HIV spread and the interrelationship of epidemics
in the different regions in Asia.
Foundation items: Grant support from Ministry of Health, Labour and Welfare and Ministry of Education, Science and Technology
in Japan; Japanese Foundation for AIDS Prevention. 相似文献
935.
936.
937.
938.
Miguel Martorell Ana Pérez-Vallés Francisco Gozalbo Jose Angel Garcia-Garcia Jair Gutierrez John Gaona 《Diagnostic pathology》2007,2(1):19
Background
Extrapleural Solitary Fibrous tumors (SFTs) have been increasingly reported. The retroperitoneum, deep soft tissues of proximal extremities, abdominal cavity, trunk, head and neck are the most common extraserosal locations reported. Microscopically they show a wide range of morphological features, and so the differential diagnosis is extensive. Immunohistochemically, they commonly express CD34, vimentin, bcl-2 and CD99. Epithelial membrane antigen (EMA) and smooth muscle actin (SMA) may occasionally be expressed. Epithelioid morphology in extrapleural SFT has only very occasionally been described (five cases reported), some of them with biphasic pattern and others with malignant characteristics.Case presentation
A SFT of the thigh with epithelioid areas in a 63 year old woman is reported. Microscopically the tumor showed areas hypo and hipercellular. At the periphery of the hipercellular areas there were nodules composed of epithelioid cells. Immunohistochemically both the spindle and epithelioid cells were positive for CD34, vimentin, bcl-2 and CD99. Epithelial, neural and muscular markers were negative. Molecular study was done and ruled out a synovial sarcoma.Conclusion
Ten cases of SFT of the thigh have been reported but to our knowledge this is the first case with epithelioid morphology affecting the extremities. Identification of this pattern of SFT is of importance, to avoid misdiagnosis with other more aggressive conditions in soft tissue.939.
940.
Peter Nyhuis Tobias Heinen Michael Brieke 《Flexible Services and Manufacturing Journal》2007,19(3):286-307
Objective-oriented factory planning is a prerequisite for the economic operation of a factory. As intensive discussions in the literature as well as practical findings in factories over the years show, transformability and logistics are among the key objectives of a factory. It is striking, however, that both objectives have not so far been related to each other. Based on these findings, a method for evaluating the actual as well as the target transformability of a factory has been developed. It allows the user to assess whether the factory possesses adequate and economic transformability. In order to make the method more manageable for users in practice, a software tool is presented, and a benchmarking has been derived from the data collected by evaluating factory transformability. In addition, it has been found that transformability can influence logistics. A procedure will be presented that allows major means of adjustment to be found that improve the logistics objectives of a factory by using transformability. Finally, the outlook for future developments is discussed. 相似文献