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61.
H. Werner Goedde Heide-G. Benkmann Ingeborg Christ Surjit Singh Ludwig Hirth 《Human genetics》1970,10(3):235-243
Summary Frequencies and phenotype distribution of red cell adenosine deaminase (ADA), adenylate kinase (AK), phosphoglucomutase (PGM1), acid phosphatase (AP) and serum 1-antitrypsin (1-at or Pi) were examined in a German group of the area of Hamburg. The observed distribution of phenotypes in all five systems is consistent with that expected by the Hardy-Weinberg equilibrium. Within the mother/child combinations we found no incompatible combination. The results of additional family investigations for 1-antitrypsin are consistent with Mendelian expectation. 相似文献
62.
Pine needle tissues were shown to incorporate acetate [1-14C] into phospho-, galacto- and neutral lipids. The major incorporation of the label among these lipids was always in the phosphatidyl choline (PC) fraction. The amount of label among the other lipid fractions varied depending on the age and source of the needle tissues (lodgepole or jack pine). In general, the biosynthesis of these lipids was more efficient in the developing than in the fully developed tissues. Treatment of the needle tissues with either gaseous or aqueous SO2 markedly inhibited their lipid biosynthesis. These effects were more pronounced in the developing than in the fully developed needles. Fumigation with gaseous SO2 showed that SO2 concentration and length of exposure determine the extent to which the lipid biosynthetic capacity of the tissues is affected. Lipid biosynthetic capacity was partially or completely recovered when plants were removed from the SO2 environment. Plants exposed to moderate SO2 concentrations (0.18–0.20 ppm) for a period of 24 hr recovered faster than those exposed to near lethal SO2 concentrations (0.34–0.37 ppm) for only 1 hr. 相似文献
63.
Anou Dreyfus Jonathan W. Dyal Raewynne Pearson Clovice Kankya Charles Kajura Lordrick Alinaitwe Steven Kakooza Katharine M. Pelican Dominic A. Travis Michael Mahero David R. Boulware Lawrence Mugisha 《PLoS neglected tropical diseases》2016,10(8)
BackgroundThe burden of human leptospirosis in Uganda is unknown. We estimated the seroprevalence of Leptospira antibodies, probable acute/recent leptospirosis, and risk factors for seropositivity in humans in rural Western Uganda.Conclusions/SignificanceThe 35% prevalence of Leptospira antibodies suggests that exposure to leptospirosis is common in rural Uganda, in particular the Nigeria serovar (Pyrogenes serogroup). Leptospirosis should be a diagnostic consideration in febrile illness and “smear-negative malaria” in rural East Africa. 相似文献
64.
Bonnie L Bullis Xiuju Li Dyal N Singh Luc G Berthiaume Larry Fliegel 《European journal of biochemistry》2002,269(19):4887-4895
The Na+/H+ exchanger is a ubiquitous membrane protein of bacteria, plants and mammals. The first isoform discovered (NHE1) is present on the mammalian plasma membrane and transports one H+ out of cells in exchange for one extracellular Na+. With solubilization in standard SDS/PAGE buffer, this protein had a high tendency to aggregate when subjected to elevated temperature. The aggregates were stable and did not dissociate in high concentrations of SDS or 2-mercaptoethanol. We examined the distribution of the Na+/H+ exchanger within membrane subfractions. The Na+/H+ exchanger was found both in caveolin-containing fractions and, in lesser amounts, in higher density membrane fractions where the bulk of proteins were contained. Treatment with cytochalasin D caused only a minor reduction of the amount of Na+/H+ exchanger present in caveolin-enriched fractions suggesting an intact cytoskeleton was not important for NHE1 localization to these microdomains. Treatment of cells with methyl beta-cyclodextrin had a small stimulatory effect on Na+/H+ exchanger activity and reduced the amount of Na+/H+ exchanger in low density membrane fractions. Our study demonstrates that SDS cannot maintain the protein in a monomeric state suggesting that strong hydrophobic interactions are responsible for this temperature dependent aggregation behavior. In addition a large proportion of the Na+/H+ exchanger protein is found to be enriched in low density caveolin-containing fractions. 相似文献
65.
66.
Mehta Alka Singh Surjit Ganguly Nirmal Kumar 《Molecular and cellular biochemistry》1999,196(1-2):175-181
Reactive oxygen species (ROS) are potent mediators of inflammatory disorders and may be of pathophysiological importance in S. typhimurium induced tissue damage. This study was carried out to investigate if ROS play a role in mediating the enterocyte damage during in vitro exposure to Salmonella typhimurium enterotoxin (S-LT). The ROS generation was detected by measuring the changes in the enterocyte arachidonic acid (AA) metabolism (measured indirectly by estimating the level of enterocyte damage in the absence and presence of the cyclooxygenase inhibitor, indomethacin) and xanthine oxidase activity. The enterocyte damage was estimated by measuring the changes in the level of lipid peroxidation and cell viability. The results obtained showed that the exposure of isolated rat enterocytes to S-LT resulted in an increased XO activity; an increased arachidonic acid metabolism, dose and time dependent increase in the level of lipid peroxidation and decreased cell viability. Lipid peroxidation decreased and cell viability increased in the presence of the antioxidant enzymes superoxide dismutase (SOD) or catalase. Thus the in vitro exposure of the enterocytes to S-LT is accompanied by an increased generation of ROS which may induce the lipid peroxidation of the enterocyte membrane thereby leading to a loss of cell viability. 相似文献
67.
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69.
H. Werner Goedde Surjit Singh Dharam P. Agarwal Gernot Fritze Kay Stapel Yong K. Paik 《Human genetics》1989,81(4):305-307
Summary Deficiency of mitochondrial aldehyde dehydrogenase (ALDH I) is an inborn error of metabolism that is responsible for acute
alcohol sensitivity (flushing response) observed only in Orientals of Mongoloid origin. Our previous studies using electrophoretic
enzyme detection have shown that this deficiency is prevalent among Japanese, Chinese, and other Orientals. We report here
the genotyping of ALDH I locus in blood samples of 218 South Korean individuals by means of hybridization analysis with allele-specific
oligonucleotide probes and enzymatically amplified human genomic DNA. The results of genotyping are compared with the phenotype
analysis in hair roots of the same individuals. Among 62 apparently deficient phenotypes, 58 heterozygote and 4 homozygote
deficient genotypes were observed. 相似文献
70.
Samantha C Lewis Leslie A Dyal Caroline F Hilburn Stephanie Weitz Wei-Siang Liau Craig W LaMunyon Dee R Denver 《BMC evolutionary biology》2009,9(1):15