排序方式: 共有67条查询结果,搜索用时 15 毫秒
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Maksimov I. V. Troshina N. B. Khairullin R. M. Surina O. B. Ganiev R. M. 《Russian Journal of Plant Physiology》2002,49(5):685-689
The growth of wheat (Triticum aestivum L., cv. Zhnitsa) seedlings and calluses infected with spores of common bunt causal agent Tilletia caries (DC) Tul. was studied. Inoculation with the pathogenic fungus enhanced both in vivo and in vitro growth due to cell division activation and cell expansion. These growth characteristics were also retained in wheat calluses infected with T. caries and grown on the hormone-free Murashige and Skoog nutrient medium. This implies the production of hormone-like substances by the fungus itself or by the infected plant. At the phase of fungal penetration into the plants, infection resulted in IAA accumulation in seedlings. Later, the IAA content reduced to a control level and the phytohormone balance was shifted toward cytokinins. Similar changes in the IAA and cytokinin levels were observed during early fungus development on wheat callus tissues. Such a sequence of events is supposed to be required for successful fungal penetration and localization in plant tissues and for the establishment of compatible interactions between the pathogen and the host plant. 相似文献
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Maksimov I. V. Cherepanova E. A. Surina O. B. Sakhabutdinova A. R. 《Russian Journal of Plant Physiology》2004,51(4):480-485
The effect of salicylic acid (SA) on peroxidase activity in wheat (Triticum aestivum L.) calli cocultured with the bunt pathogen Tilletia caries was studied. Fungal infection was shown to activate cytoplasmic peroxidase. SA suppressed total peroxidase activity but did not inhibit the peroxidase with pI 9.8. A novel chitin-specific peroxidase with pI 3.5 appeared after the SA treatment. The infection of SA-treated cells with Tilletia caries activated the isoenzymes with pI 3.5, 4.8, and 7.5 and stimulated their secretion into the culture medium. The ability of SA to control wheat peroxidase activity during pathogenesis is discussed. The important role of this control in plant defense responses to the bunt pathogen is emphasized. 相似文献
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Olga V. Mitkevich Natalia V. Kochneva-Pervukhova Elizaveta R. Surina Sergei V. Benevolensky Vitaly V. Kushnirov Michael D. Ter-Avanesyan 《朊病毒》2012,6(4):400-406
Amyloids are fibrillar protein aggregates resulting from non-covalent autocatalytic polymerization of various structurally and functionally unrelated proteins. Previously we have selected DNA aptamers, which bind specifically to the in vitro assembled amyloid fibrils of the yeast prionogenic protein Sup35. Here we show that such DNA aptamers can be used to detect SDS-insoluble amyloid aggregates of the Sup35 protein, and of some other amyloidogenic proteins, including mouse PrP, formed in yeast cells. The obtained data suggest that these aggregates and the Sup35 amyloid fibrils assembled in vitro possess common conformational epitopes recognizable by aptamers. The described DNA aptamers may be used for detection of various amyloid aggregates in yeast and, presumably, other organisms. 相似文献
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I. V. Maksimov N. B. Troshina O. B. Surina E. A. Cherepanova L. G. Yarullina 《Applied Biochemistry and Microbiology》2010,46(5):530-535
The effect of Ca2+ on morphophysiological parameters of wheat calli (Triticum aestivum L.) infected by the bunt pathogen Tilletia caries, in particular on the level of active oxygen species, activity of oxalate oxidase, peroxidase, and catalase is investigated.
The concentration of O2−, H2O2, and activity of oxidoreductases (oxalate oxidase, peroxidase, and catalase) depended on the content of Ca2+ in the culture medium of calli. The increase of the concentration of Ca2+ ions in the culture medium led to forming of calli with high structure, induction of activity of oxalate oxidase and of some
isoperoxidase, and to accumulation of active oxygen species. These changes contributed to inhibition of development of the
fungus. So this dependence confirm the role of calcium as the intermediant in biochemical reactions related to the formation
of the protective response of plant cells to biotic stress. 相似文献
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Surina N. M. Fedotova I. B. Poletaeva I. I. 《Journal of Evolutionary Biochemistry and Physiology》2022,58(4):1110-1118
Journal of Evolutionary Biochemistry and Physiology - Dexamethasone is a synthetic glucocorticosteroid with anti–inflammatory and immunosuppressive effects. In order to identify new targets... 相似文献
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The hybrids between Krushinsky-Molodkina (KM) inbred strain, selected for high predisposition to audiogenic epilepsy (AE), and Wistar rats non-prone to audiogenic seizure were the initial population for selection. Rats were selected for the trait "the absence of audiogenic seizure proneness". The creation of such strain in which the significant proportion of animals develop no AE in response to sound and share partly the genetic background of the KM strain is very important for the correct use of RV strain as the laboratory model of seizure states. As alleles which determine the AE proneness are recessive the selection for the "opposite" trait proceeds necessarily slow. 相似文献