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91.
Prajisha Jayaprakash Jayashree Biswal Sureka Kanagarajan Dhamodharan Prabhu Prerana Gogoi Shankar Prasad Kanaujia 《Journal of receptor and signal transduction research》2019,39(1):28-38
Vanishing white matter (VWM) is a hereditary human disease, mostly prevalent in childhood caused by the defects in the eukaryotic initiation factor beta subunits. It is the first disease involved in the translation initiation factor, eIF2B. There is no specific treatment for VWM which mainly affect the brain and ovaries. The gray matter remains normal in all characteristics while the white matter changes texture, coming to the pathophysiology, many initiation factors are involved in the initiation of translation of mRNAs into polypeptides. In this study, the three-dimensional structure of PhMTNA protein was modeled and the stability ascertained through Molecular dynamic simulation (MDS) for 100?ns. The active site residues are conserved with the reported BsMTNA structure which is also confirmed through sitemap prediction. Through virtual screening and induced fit docking, top five leads against PhMTNA protein was identified based on their binding mode and affinity. ADME properties and DFT (Density Functional Theory) studies of these compounds were studied. In addition to that, computational mutagenesis studies were performed to identify the hotspot residues involved in the protein-ligand interactions. Overall analysis showed that the compound NCI_941 has a highest binding energy of ?46.256?kcal mol?1 in the Arg57Ala mutant. Thus, the results suggest that NCI_941 would act as a potent inhibitor against PhMTNA protein. 相似文献
92.
Muthukumarasamy Uthayakumar Bowdadu Benazir Sanjeev Patra Marthandan Kirti Vaishnavi Manickam Gurusaran Kanagarajan Sureka Jeyaraman Jeyakanthan Kanagaraj Sekar 《基因组蛋白质组与生物信息学报(英文版)》2012,10(4):217-225
Analysis of protein sequences from Mycobacterium tuberculosis H37Rv(Mtb H37Rv) was performed to identify homopeptide repeatcontaining proteins(HRCPs).Functional annotation of the HRCPs showed that they are preferentially involved in cellular metabolism.Furthermore,these homopeptide repeats might play some specific roles in protein-protein interaction.Repeat length differences among Bacteria,Archaea and Eukaryotes were calculated in order to identify the conservation of the repeats in these divergent kingdoms.From the results,it was evident that these repeats have a higher degree of conservation in Bacteria and Archaea than in Eukaryotes.In addition,there seems to be a direct correlation between the repeat length difference and the degree of divergence between the species.Our study supports the hypothesis that the presence of homopeptide repeats influences the rate of evolution of the protein sequences in which they are embedded.Thus,homopeptide repeat may have structural,functional and evolutionary implications on proteins. 相似文献
93.
Ganesh Kumar Arumugam Sekaran Ganesan Swarnalatha Somasundaram Prasad Rao Burusa 《World journal of microbiology & biotechnology》2005,21(6-7):999-1007
Summary The solid resinous product (SRP) containing unsaturated/saturated dicarboxylic acid residues, phthalic acid and maleic acid
is discharged as a solid waste during cracking of benzene over vanadium at temperatures above 500°C in the dicarboxylic acid
manufacturing industry. In the present study the solid waste was diluted with water to a concentration of 0.5% w/v for microbial
degradation. The waste was fermented in a reactor containing mesoporous activated carbon on which was immobilized Saccharomyces cerevisiae at an optimum residence time of 24 h at pH 6.5. The immobilized-yeast-treated samples were further treated in an upflow anaerobic
reactor at an hydraulic retention time (HRT) of 0.1038 days at a hydraulic flow rate of 7.34 × 10−3 m3/day and chemical oxygen demand (COD) loading rate of 2.19 kg/m3/day. The pathway followed in the degradation of dicarboxylic acid into end products by anaerobic metabolism in the yeast
cell fermentor and in the upflow anaerobic reactor was confirmed through HPLC, Fourier transform infra red spectroscopy and
proton and 13C NMR spectroscopy. 相似文献