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361.
A total of 169 children with primary enuresis were treated by one of three methods--use of a special diet, imipramine, or a waking device (the Mozes detector). Of 64 who received the diet 1 (1.6%) was cured; of 62 who were treated with imipramine 13 (20.9%) were cured; and of 43 who used the detector 23 (53.5%) were cured. Results at follow-up remained substantially unchanged for cure. Improvement rate without cure during treatment was higher for the children treated with imipramine than for those treated with the detector, but at follow-up the reverse was true. Parents of children who received the special diet, because they were unimpressed by the rate of improvement, would not permit their children to continue this form of therapy for longer than 1 or 2 months. 相似文献
362.
Surya C. Gnyawali Sashwati Roy Jason Driggs Savita Khanna Thomas Ryan Chandan K. Sen 《Journal of visualized experiments : JoVE》2010,(41)
Ischemia-reperfusion (IR) was surgically performed in murine hearts which were then subjected to repeated imaging to monitor temporal changes in functional parameters of key clinical significance. Two-dimensional movies were acquired at high frame rate (8 kHz) and were utilized to estimate high-quality myocardial strain. Two-dimensional elastograms (strain images), as well as strain profiles, were visualized. Results were powerful in quantitatively assessing IR-induced changes in cardiac events including left-ventricular (LV) contraction, LV relaxation and isovolumetric phases of both pre-IR and post-IR beating hearts in intact mice. In addition, compromised sector-wise wall motion and anatomical deformation in the infarcted myocardium were visualized. The elastograms were uniquely able to provide information on the following parameters in addition to standard physiological indices that are known to be affected by myocardial infarction in the mouse: internal diameters of mitral valve orifice and aorta, effective regurgitant orifice, myocardial strain (circumferential as well as radial), turbulence in blood flow pattern as revealed by the color Doppler movies and velocity profiles, asynchrony in LV sector, and changes in the length and direction of vectors demonstrating slower and asymmetrical wall movement. This work emphasizes on the visual demonstration of how such analyses are performed. 相似文献
363.
Rajnish Khanna ;Junlin Li ;Tong-Seung Tseng ;Julian I. Schroeder ;David W. Ehrhardt ;Winslow R. Briggs 《植物生理与分子生物学学报》2014,(9):1441-1454
Reorganization of the cortical microtubule cytoskeleton is critical for guard cell function. Here, we investigate how environmental and hormonal signals cause these rearrangements and find that COP1, a RING-finger-type ubiquitin E3 ligase, is required for degradation of tubulin, likely by the 26S proteasome. This degradation is required for stomatal closing. In addition to regulating the cytoskeleton, we show that cop1 mutation impaired the activity of S-type anion channels, which are critical for stomatal closure. Thus, COP1 is revealed as a potential coordinator of cytoskeletal and electrophysiological activities required for guard cell function. 相似文献
364.
Kristopher T Kahle Nancy D Merner Perrine Friedel Liliya Silayeva Bo Liang Arjun Khanna Yuze Shang Pamela Lachance‐Touchette Cynthia Bourassa Annie Levert Patrick A Dion Brian Walcott Dan Spiegelman Alexandre Dionne‐Laporte Alan Hodgkinson Philip Awadalla Hamid Nikbakht Jacek Majewski Patrick Cossette Tarek Z Deeb Stephen J Moss Igor Medina Guy A Rouleau 《EMBO reports》2014,15(7):766-774
The KCC2 cotransporter establishes the low neuronal Cl− levels required for GABAA and glycine (Gly) receptor-mediated inhibition, and KCC2 deficiency in model organisms results in network hyperexcitability. However, no mutations in KCC2 have been documented in human disease. Here, we report two non-synonymous functional variants in human KCC2, R952H and R1049C, exhibiting clear statistical association with idiopathic generalized epilepsy (IGE). These variants reside in conserved residues in the KCC2 cytoplasmic C-terminus, exhibit significantly impaired Cl−-extrusion capacities resulting in less hyperpolarized Gly equilibrium potentials (EGly), and impair KCC2 stimulatory phosphorylation at serine 940, a key regulatory site. These data describe a novel KCC2 variant significantly associated with a human disease and suggest genetically encoded impairment of KCC2 functional regulation may be a risk factor for the development of human IGE. 相似文献
365.
Malini R. Capoor Geetika Khanna Rajni Kadambari Batra Deepthi Nair V. P. Venkatchalam Pushpa Aggarwal 《Mycopathologia》2009,168(2):89-94
We report three cases of rhinosporidiosis from migrant population of Delhi. Three male patients had sino-nasopharyngeal, nasopharyngeal
and nasal rhinosporidiosis, respectively. One patient gave a history of bathing in stagnant water. The diagnosis was made
by clinical presentation and microscopic observation of characteristic sporangia of Rhinosporidium seebri in mycological and histopathological investigations. All the patients were successfully treated with complete surgical excision
of lesions and cauterization of base. There were no recurrences. 相似文献
366.
Biodegradation of Chlorpyrifos in Soil by Enriched Cultures 总被引:3,自引:0,他引:3
Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade
chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days
by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsiella sp., and Serratia marscecens obtained from these consortia showed 84, 84, 81, and 80% degradation of chlorpyrifos (50 mg/L) in liquid medium after 20 days
and 92, 60, 56, and 37% degradation of chlorpyrifos (50 mg/L) in soil after 30 days. Populations of Bacillus cereus, Klebsiella sp., and Serratia marscecens remained steady in soil experiments except for P. aeruginosa, where the population showed a substantial increase. Formation of 3,5,6-trichloro-2-pyridinol, the major metabolite of chlorpyrifos
degradation, was observed during the degradation of chlorpyrifos by P. aeruginosa, which disappeared to negligible amounts. 相似文献
367.
Martin Mendoza Chand Khanna 《The international journal of biochemistry & cell biology》2009,41(7):1452-1462
Metastasis remains the overwhelming cause of death for cancer patients. During metastasis, cancer cells will leave the primary tumor, intravasate into the bloodstream, arrest at a distant organ, and eventually develop into gross lesions at the secondary sites. This intricate process is influenced by innumerable factors and complex cellular interactions described in 1889 by Stephen Paget as the seed and soil hypothesis. In this review, we revisit this seed and soil hypothesis with an emerging understanding of the cancer cell (i.e. seed) and its microenvironment (i.e. soil). We will provide background to suggest that a critical outcome of the seed–soil interaction is resistance of the stresses that would otherwise impede metastasis. 相似文献
368.
Chandrasekhar Gurramkonda Ahmad Adnan Thomas Gäbel Heinrich Lünsdorf Anton Ross Satish Kumar Nemani Sathyamangalam Swaminathan Navin Khanna Ursula Rinas 《Microbial cell factories》2009,8(1):13-8
Background
Hepatitis B is a serious global public health concern. Though a safe and efficacious recombinant vaccine is available, its use in several resource-poor countries is limited by cost. We have investigated the production of Hepatitis B virus surface antigen (HBsAg) using the yeast Pichia pastoris GS115 by inserting the HBsAg gene into the alcohol oxidase 1 locus. 相似文献369.
Saxena S Pant AB Khanna VK Singh K Shukla RK Meyer CH Singh VK 《Journal of ocular biology, diseases, and informatics》2010,3(1):35-38
Tumor necrosis factor-alpha (TNF-α) is a pleiotropic inflammatory cytokine. Tumor necrosis factor-alpha was evaluated in the serum samples of patients with idiopathic retinal periphlebitis in young adults (Eales’ disease). Retinal periphlebitis was graded according to a new grading system based on severity of inflammation (grade 1–4). Quantification of the TNF-α levels was carried out using ELISA kit in the serum samples of young adults with idiopathic retinal periphlebitis (n = 17) and healthy controls (n = 17) of similar age. Tumor necrosis factor-α level was found to be significantly raised in cases with retinal periphlebitis as compared with controls (p < 0.001). Higher levels of TNF-α were found to be associated with increased severity of retinal periphlebitis. Tumor necrosis factor-α represents a novel target for controlling inflammatory activity in idiopathic retinal periphlebitis. Higher levels of TNF-α, in association with the increased severity of retinal periphlebitis, have implications for early anti-TNF-α therapy. 相似文献
370.
Henderson MJ Munoz MA Saunders DN Clancy JL Russell AJ Williams B Pappin D Khanna KK Jackson SP Sutherland RL Watts CK 《The Journal of biological chemistry》2006,281(52):39990-40000
EDD, the human orthologue of Drosophila melanogaster "hyperplastic discs," is overexpressed or mutated in a number of common human cancers. Although EDD has been implicated in DNA damage signaling, a definitive role has yet to be demonstrated. Here we report a novel interaction between EDD and the DNA damage checkpoint kinase CHK2. EDD and CHK2 associate through a phospho-dependent interaction involving the CHK2 Forkhead-associated domain and a region of EDD spanning a number of putative Forkhead-associated domain-binding threonines. Using RNA interference, we demonstrate a critical role for EDD upstream of CHK2 in the DNA damage signaling pathway. EDD is necessary for the efficient activating phosphorylation of CHK2 in response to DNA damage following exposure to ionizing radiation or the radiomimetic, phleomycin. Cells depleted of EDD display impaired CHK2 kinase activity and an inability to respond to DNA damage. These results identify EDD as a novel mediator in DNA damage signal transduction via CHK2 and emphasize the potential importance of EDD in cancer. 相似文献